Abstract
A previous study of 76 plant species on Spitsbergen in the High Arctic concluded that structures resembling arbuscular mycorrhizas were absent from roots. Here, we report a survey examining the roots of 13 grass and forb species collected from 12 sites on the island for arbuscular mycorrhizal (AM) colonisation. Of the 102 individuals collected, we recorded AM endophytes in the roots of 41 plants of 11 species (Alopecurus ovatus, Deschampsia alpina, Festuca rubra ssp. richardsonii, putative viviparous hybrids of Poa arctica and Poa pratensis, Poa arctica ssp. arctica, Trisetum spicatum, Coptidium spitsbergense, Ranunculus nivalis, Ranunculus pygmaeus, Ranunculus sulphureus and Taraxacum arcticum) sampled from 10 sites. Both coarse AM endophyte, with hyphae of 5–10 μm width, vesicles and occasional arbuscules, and fine endophyte, consisting of hyphae of 1–3 μm width and sparse arbuscules, were recorded in roots. Coarse AM hyphae, vesicles, arbuscules and fine endophyte hyphae occupied 1.0–30.7, 0.8–18.3, 0.7–11.9 and 0.7–12.8% of the root lengths of colonised plants, respectively. Principal component analysis indicated no associations between the abundances of AM structures in roots and edaphic factors. We conclude that the AM symbiosis is present in grass and forb roots on Spitsbergen.
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Allen SE (1989) Chemical analysis of ecological materials, 2nd edn. Blackwell, Oxford, p 368
Allen N, Nordlander M, McGonigle T, Basinger J, Kaminskyj S (2006) Arbuscular mycorrhizae on Axel Heiberg Island (80 °N) and at Saskatoon (52 °N) Canada. Can J Bot 84:1094–1100
Alsos IG, Westergaard K, Lund L, Sandbakk BE (2004) The flora of Colesdalen, Svalbard. Blyttia 62:142–151
Evans DG, Miller MH (1988) Vesicular-arbuscular mycorrhizas and the soil-disturbance-induced reduction of nutrient absorption in maize. I. Causal relations. New Phytol 110:67–74
Helgason T, Fitter AH (2009) Natural selection and the evolutionary ecology of the arbuscular mycorrhizal fungi (phylum Glomeromycota). J Exp Bot 60:2465–2480
Isaksen K, Nordli Ø, Førland EJ, Łupikasza E, Eastwood S, Niedźwiedź T (2016) Recent warming on Spitsbergen—influence of atmospheric circulation and sea ice cover. J Geophys Res Atmos 121:11913–11931
Joly D, Nilsen L, Brossard T, Elvebakk A (2010) Plants as bioindicator for temperature interpolation purposes: analyzing spatial correlation between botany based index of thermophily and integrated temperature characteristics. Ecol Indic 10:990–998
Lid J, Lid DT (2005) Norsk flora, 7th edn. Det Norske Samlaget, Oslo, p 1230
Liu M, Zheng R, Bai S, Bai Y, Wang J (2017) Slope aspect influences arbuscular mycorrhizal fungus communities in arid ecosystems of Daqingshan Mountains, Inner Mongolia, North China. Mycorrhiza 27:189–200
McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swann JA (1990) A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol 155:495–501
Mullen RB, Schmidt SK (1993) Mycorrhizal infection, phosphorus uptake, and phenology in Ranunculus adoneus: implications for the functioning of mycorrhizae in alpine systems. Oecologia 94:229–234
Newsham KK, Upson R, Read DJ (2009) Mycorrhizas and dark septate root endophytes in cold regions. Fungal Ecol 2:10–20
Oksanen J, Blanchet FG, Friendly M, Kindt R, Legendre P, McGlinn D, Minchin PR, O'Hara RB, Simpson GL, Solymos P, Stevens MHH, Szoecs E, Wagner H (2017) Vegan: Community Ecology Package. R package version 2.4–3. https://CRAN.R-project.org/package=vegan
Olsson PA, Eriksen B, Dahlberg A (2004) Colonization by arbuscular mycorrhizal and fine endophytic fungi in herbaceous vegetation in the Canadian High Arctic. Can J Bot 82:1547–1556
Öpik M, Zobel M, Cantero JJ, Davison J, Facelli JM, Hiiesalu I, Jairus T, Kalwij JM, Koorem K, Leal ME, Liira J, Metsis M, Neshataeva V, Paal J, Phosri C, Reier U, Thiery O, Vasar M, Moora M (2013) Global sampling of plant roots expands the described molecular diversity of arbuscular mycorrhizal fungi. Mycorrhiza 23:411–430
Orchard S, Hilton S, Bending GD, Dickie IA, Standish RJ, Gleeson D, Jeffery RP, Powell JR, Walker C, Bass D, Monk J, Simonin A, Ryan MH (2017) Fine endophytes (Glomus tenue) are related to Mucoromycotina, not Glomeromycota. New Phytol 213:481–486
Ormsby A, Hodson E, Li Y, Basinger J, Kaminskyj S (2007) Quantitation of endorhizal fungi in High Arctic tundra ecosystems through space and time: the value of herbarium archives. Can J Bot 85:599–606
Pryzbylak R (1992) Spatial differentiation of air temperature and humidity on western coast of Spitsbergen in 1979–1983. Polish Polar Res 13:113–129
Read DJ (1991) Mycorrhizas in ecosystems. Experientia 47:376–391
Smith SE, Read DJ (2008) Mycorrhizal Symbiosis, 3rd edn. Academic, London
Spatafora JW, Chang Y, Benny GL, Lazarus K, Smith ME, Berbee ML, Bonito G, Corradi N, Grigoriev I, Gryganskyi A, James TY, O'Donnell K, Roberson RW, Taylor TN, Uehling J, Vilgalys R, White MM, Stajich JE (2016) A phylum-level phylogenetic classification of zygomycete fungi based on genome-scale data. Mycologia 108:1028–1046
Tibbett M, Cairney JWG (2007) The cooler side of mycorrhizas: their occurrence and functioning at low temperatures. Can J Bot 85:51–62
Upson R, Newsham KK, Read DJ (2008) Root-fungal associations of Colobanthus quitensis and Deschampsia antarctica in the maritime and sub-Antarctic. Arct Antarct Alp Res 40:592–599
Väre H, Vestberg M, Eurola S (1992) Mycorrhiza and root associated fungi in Spitsbergen. Mycorrhiza 1:93–104
Wang B, Qui Y-L (2006) Phylogenetic distribution and evolution of mycorrhizas in land plants. Mycorrhiza 16:299–363
Acknowledgments
The University Centre in Svalbard (UNIS) provided financial support for this study through its Arctic Mycology course (AB-336/836). The UNIS Logistics Department and Nick Cox of the UK Arctic Research Station provided boat access to Kapp Linné, Colesdalen, Kvadehuken and Ossian Sars. The Governor of Svalbard permitted the collection of plants and soils, and Professor Steve Coulson gave advice on sampling locations. Iva Pitelkova and Professors Ulrik Søchting and Kirsten Christoffersen helped to collect plants and soils, Laura Gerrish drew the map shown in Online Resource 1 and Katherine Francis helped to prepare soil samples. Manisha Patel of CEH Lancaster performed P determinations, and Professor Henry Väre (University of Helsinki) provided helpful information. The editor and two anonymous reviewers provided helpful comments. All are gratefully acknowledged.
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Newsham, K.K., Eidesen, P.B., Davey, M.L. et al. Arbuscular mycorrhizas are present on Spitsbergen. Mycorrhiza 27, 725–731 (2017). https://doi.org/10.1007/s00572-017-0785-9
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DOI: https://doi.org/10.1007/s00572-017-0785-9