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Colonization and community structure of arbuscular mycorrhizal fungi in maize roots at different depths in the soil profile respond differently to phosphorus inputs on a long-term experimental site

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Abstract

Effects of soil depth and plant growth stages on arbuscular mycorrhizal fungal (AMF) colonization and community structure in maize roots and their potential contribution to host plant phosphorus (P) nutrition under different P-fertilizer inputs were studied. Research was conducted on a long-term field experiment over 3 years. AMF colonization was assessed by AM colonization rate and arbuscule abundances and their potential contribution to host P nutrition by intensity of fungal alkaline phosphatase (ALP)/acid phosphatase (ACP) activities and expressions of ZmPht1;6 and ZmCCD8a in roots from the topsoil and subsoil layer at different growth stages. AMF community structure was determined by specific amplification of 18S rDNA. Increasing P inputs up to 75–100 kg ha−1 yr−1 increased shoot biomass and P content but decreased AMF colonization and interactions between AMF and roots. AM colonization rate, intensity of fungal ACP/ALP activities, and expression of ZmPht1;6 in roots from the subsoil were greater than those from topsoil at elongation and silking but not at the dough stage when plants received adequate or excessive P inputs. Neither P input nor soil depth influenced the number of AMF operational taxonomic units (OTUs) present in roots, but P-fertilizer input, in particular, influenced community composition and relative AMF abundance. In conclusion, although increasing P inputs reduce AMF colonization and influence AMF community structure, AMF can potentially contribute to plant P nutrition even in well-fertilized soils, depending on the soil layer in which roots are located and the growth stage of host plants.

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Acknowledgements

C.W. and C.L.’s research is supported by grants from the State Key Basic Research and Development Plan of China (No. 2013CB127402) and the Innovative Group Grant of the National Natural Science Foundation of China (NSFC) (No. 31421092). P.J.W.’s research is supported by the Rural and Environmental Science and Analytical Services Division (RESAS) of the Scottish Government Strategic Programme (2016–2021).

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  1. Department of Plant Nutrition, College of Resources and Environmental Science, China Agricultural University, Yuanmingyuan West Road 2, Beijing, 100193, People’s Republic of China

    Chao Wang & Chunjian Li

  2. Ecological Sciences, The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK

    Philip J. White

  3. Distinguished Scientist Fellowship Program, King Saud University, Riyadh, Saudi Arabia

    Philip J. White

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  1. Chao Wang
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Wang, C., White, P.J. & Li, C. Colonization and community structure of arbuscular mycorrhizal fungi in maize roots at different depths in the soil profile respond differently to phosphorus inputs on a long-term experimental site. Mycorrhiza 27, 369–381 (2017). https://doi.org/10.1007/s00572-016-0757-5

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