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Arbuscular mycorrhizal fungal community differences among European long-term observatories

Mycorrhiza volume 27, pages 331–343 (2017)Cite this article

Abstract

Arbuscular mycorrhizal fungal (AMF) communities have been demonstrated to respond to a variety of biotic and abiotic factors, including various aspects of land management. Numerous studies have specifically addressed the impact of land use on AMF communities, but usually have been confined to one or a few sites. In this study, soil AMF assemblages were described in four different long-term observatories (LTOs) across Europe, each of which included a site-specific high-intensity and a low-intensity land use. AMF communities were characterized on the basis of 454 sequencing of the internal transcribed spacer 2 (ITS2) rDNA region. The primary goals of this study were (i) to determine the main factors that shape AMF communities in differentially managed sites in Europe and (ii) to identify individual AMF taxa or combinations of taxa suitable for use as biomarkers of land use intensification. AMF communities were distinct among LTOs, and we detected significant effects of management type and soil properties within the sites, but not across all sites. Similarly, indicator species were identified for specific LTOs and land use types but not universally for high- or low-intensity land uses. Different subsets of soil properties, including several chemical and physical variables, were found to be able to explain an important fraction of AMF community variation alone or together with other examined factors in most sites. The important factors were different from those for other microorganisms studied in the same sites, highlighting particularities of AMF biology.

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Acknowledgments

We are grateful to Alberto Orgiazzi (EU-commission, JRC) for his useful suggestions. We warmly acknowledge the LTO leaders and their institutions for experimental facilities and access to data: P.P. Roggero (University of Sassari, Italy), R. Bardgett (University of Lancaster, United Kingdom), A. Chabbi (INRA, Lusignan, France), R. Finlay (SLU, Uppsala, Sweden), W. van der Putten (NIOO, Wageningen, The Netherlands). This work was supported by the European Commission project EcoFINDERS (FP7-64465; Ecological Function and Biodiversity Indicators in European Soils, http://ecofinders.eu/).

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Author notes

  1. M.-L. Bouffaud

    Present address: Helmholtz Center for Environmental Research – UFZ, Halle, Germany, German Centre for Integrative Biodiversity Research (iDiv), Leipzig, Germany

Affiliations

  1. Agroécologie, AgroSup Dijon, CNRS, INRA, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France

    M.-L. Bouffaud, M. Peyret-Guzzon, H. Stockinger, D. van Tuinen, D. Wipf & D. Redecker

  2. Department of Life Sciences and System Biology (DBios), University of Torino, 25 Viale Mattioli, 10125, Torino, Italy

    C. Bragalini, S. Voyron & M. Girlanda

  3. Université Claude Bernard Lyon1, Lyon, France

    C. Bragalini & M. Girlanda

  4. CNR-Istituto per la Protezione Sostenibile delle Piante, UOS Turin (CNR-IPSP), Torino, Italy

    A. Berruti, E. Lumini & V. Bianciotto

  5. Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne Franche-Comté, F-21000, Dijon, France

    P. Plassart & P. Lemanceau

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  1. M.-L. Bouffaud
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M.-L. Bouffaud and C. Bragalini contributed equally to the work.

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Figure S1:

Species accumulation curve per sample. (PDF 337 kb)

Figure S2:

PCA biplot of the soil properties data (combined dataset: spring + autumn). Arrows represent the direction and effect of each particular soil variable in discriminating between soil from different LTOs. H, high intensity land use; L., low intensity land use. (PDF 208 kb)

Figure S3:

Percentages of total sequences assigned to a particular Glomeromycota order for each LTO intensification level and time. Numbers of sequences and results of the statistical tests are reported in (PDF 142 kb)

Figure S4:

Boxplot of multivariate homogeneity of groups’ dispersions (betadisper) of samples based on AMF community composition analyzed according to LTO (A) and intensification level (B). Different letters indicate significant (P < 0.05) differences between groups (Tukey’s HSD test). H, high intensity land use; L., low intensity land use. (PDF 137 kb)

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Bouffaud, ML., Bragalini, C., Berruti, A. et al. Arbuscular mycorrhizal fungal community differences among European long-term observatories. Mycorrhiza 27, 331–343 (2017). https://doi.org/10.1007/s00572-016-0753-9

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Keywords

  • Arbuscular mycorrhizal fungi
  • Long-term observatories
  • 454 pyrosequencing
  • ITS2
  • Soil management intensity