Are there keystone mycorrhizal fungi associated to tropical epiphytic orchids?
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In epiphytic orchids, distinctive groups of fungi are involved in the symbiotic association. However, little is known about the factors that determine the mycorrhizal community structure. Here, we analyzed the orchid mycorrhizal fungi communities associated with three sympatric Cymbidieae epiphytic tropical orchids (Cyrtochilum flexuosum, Cyrtochilum myanthum, and Maxillaria calantha) at two sites located within the mountain rainforest of southern Ecuador. To characterize these communities at each orchid population, the ITS2 region was analyzed by Illumina MiSeq technology. Fifty-five mycorrhizal fungi operational taxonomic units (OTUs) putatively attributed to members of Serendipitaceae, Ceratobasidiaceae and Tulasnellaceae were identified. Significant differences in mycorrhizal communities were detected between the three sympatric orchid species as well as among sites/populations. Interestingly, some mycorrhizal OTUs overlapped among orchid populations. Our results suggested that populations of studied epiphytic orchids have site-adjusted mycorrhizal communities structured around keystone fungal species. Interaction with multiple mycorrhizal fungi could favor orchid site occurrence and co-existence among several orchid species.
KeywordsEpiphytic tropical orchids Co-existing species Illumina sequencing ITS2 Microbial community assembly OTU reconstruction
This research was supported by the Académie de Recherche et d’Enseignement Supérieur Wallonie-Bruxelles (ARES) within the frame of a PRD project. We would like to thank Paulo Herrera for his contribution to the laboratory work.
- Abarenkov K, Tedersoo L, Nilsson RH, Vellak K, Saar I, Veldre V, Parmasto E, Prous M, Aan A, Ots M et al (2010b) Plutof-a web based workbench for ecological and taxonomic research, with an online implementation for fungal ITS sequences. Evol Bioinform 6:189–196Google Scholar
- Colwell RK (2013) EstimateS: statistical estimation of species richness and shared species from samples. Version 9. User’s Guide and application published at: http://purl.oclc.org/estimates
- Jacquemyn H, Waud M, Lievens B, Brys R (2016) Differences in mycorrhizal communities between Epipactis palustris, E. helleborine and its presumed sister species E. neerlandica. Annals of Botany: mcw015Google Scholar
- Jiménez-Valverde A, Hortal J (2003) Las curvas de acumulación de especies y la necesidad de evaluar la calidad de los inventarios biológicos. Revista Ibérica de Aracnología 8:151–161Google Scholar
- Kottke I, Setaro S, Haug I, Herrera P, Cruz D, Fries A, Gawlik J, Homeier J, Werner FA, Gerique A et al (2013) Mycorrhiza networks promote biodiversity and stabilize the tropical mountain rain forest ecosystem: perspectives for understanding complex communities. In: Bendix J, Beck E, Bräuning A, Makeschin F, Mosandl R, Scheu S, Wilcke W (eds) Ecosystem services, biodiversity and environmental change in a tropical mountain ecosystem of South Ecuador. Springer, Berlin, Germany, pp. 187–203CrossRefGoogle Scholar
- Oksanen AJ, Blanchet FG, Kindt R, Legendre P, Minchin PR, Hara RBO, Simpson GL, Solymos P, Stevens MHH, Wagner H (2016) Vegan: community ecology package. R Package Version 2:3–5Google Scholar
- Pellegrino G, Luca A, Bellusci F (2014) Relationships between orchid and fungal biodiversity: mycorrhizal preferences in Mediterranean orchids. Plant Biosyst 3504:1–10Google Scholar
- R Core Team (2015) R: A language and environment for statistical computing. Vienna, Austria.Google Scholar
- Smith SE, Read D (2008) Mycorrhizal symbiosis. Academic Press, New York, USAGoogle Scholar
- Suárez JP, Eguiguren S, Herrera P, Jost L (2016) Do mycorrhizal fungi drive speciation in Teagueia (orchidaceae) in the upper Pastaza watershed of Ecuador? Symbiosis 36:135–136Google Scholar
- Turenne C, Sanche S, Hoban D, Karlowsky J, Kabani A (1999) Rapid identification of fungi by using the ITS2 genetic region and an automated fluorescent capillary electrophoresis system. J Clin Microbiol 37:1846–1851Google Scholar
- White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomial RNA genes for phyologenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, San DiegoGoogle Scholar