, Volume 26, Issue 6, pp 575–585 | Cite as

Organic amendments increase phylogenetic diversity of arbuscular mycorrhizal fungi in acid soil contaminated by trace elements

  • María del Mar Montiel-RozasEmail author
  • Álvaro López-García
  • Rasmus Kjøller
  • Engracia Madejón
  • Søren Rosendahl
Original Article


In 1998, a toxic mine spill polluted a 55-km2 area in a basin southward to Doñana National Park (Spain). Subsequent attempts to restore those trace element-contaminated soils have involved physical, chemical, or biological methodologies. In this study, the restoration approach included application of different types and doses of organic amendments: biosolid compost (BC) and leonardite (LEO). Twelve years after the last addition, molecular analyses of arbuscular mycorrhizal (AM) fungal communities associated with target plants (Lamarckia aurea and Chrysanthemum coronarium) as well as analyses of trace element concentrations both in soil and in plants were performed. The results showed an improved soil quality reflected by an increase in soil pH and a decrease in trace element availability as a result of the amendments and dosages. Additionally, the phylogenetic diversity of the AM fungal community increased, reaching the maximum diversity at the highest dose of BC. Trace element concentration was considered the predominant soil factor determining the AM fungal community composition. Thereby, the studied AM fungal community reflects a community adapted to different levels of contamination as a result of the amendments. The study highlights the long-term effect of the amendments in stabilizing the soil system.


Trace element contaminated soils Ecosystem restoration Soil biodiversity Mine spill Soil fungal community Bioindicator 



AGL2011-23617 project was supported by the CICYT of the Ministerio de Ciencia e Innovación of Spain and FEDER (EU). M Mar Montiel-Rozas acknowledges support from the Ministerio de Economía y Competitividad (FPI grant, BES-2012-05339).

Supplementary material

572_2016_694_MOESM1_ESM.docx (1024 kb)
Online Resource 1 Distribution of amended plots in the experimental field and location inside of the area affected by mine spill. (DOCX 1023 kb)
572_2016_694_MOESM2_ESM.docx (1 mb)
Online Resource 2 NeighborNet split network on the site dataset based on the Monophyletic Clade Approach. (DOCX 1025 kb)
572_2016_694_MOESM3_ESM.docx (1024 kb)
Online Resource 3 Relative abundance of OTUs identified in each treatment. (DOCX 1023 kb)
572_2016_694_MOESM4_ESM.docx (1 mb)
Online Resource 4 Permutational multivariate analysis of the effect of amendment treatments, plant host identity and spatial processes on arbuscular mycorrhizal community composition. (DOCX 1024 kb)
572_2016_694_MOESM5_ESM.docx (1023 kb)
Online Resource 5 Principal Component Analysis (PCA) of available trace elements in the experimental area. (DOCX 1023 kb)


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Further Reading

  1. Huson DH, Rupp R, Scornavacca C (2011) Phylogenetic networks: concepts, algorithms and applications. Cambridge University Press, CambridgeGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • María del Mar Montiel-Rozas
    • 1
    Email author
  • Álvaro López-García
    • 2
  • Rasmus Kjøller
    • 2
  • Engracia Madejón
    • 1
  • Søren Rosendahl
    • 2
  1. 1.Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS-CSIC) Avda. Reina MercedesSevillaSpain
  2. 2.Department of BiologyUniversity of CopenhagenCopenhagen ØDenmark

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