Genomic insights into the carbohydrate catabolism of Cairneyella variabilis gen. nov. sp. nov., the first reports from a genome of an ericoid mycorrhizal fungus from the southern hemisphere
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This paper describes a novel species of ericoid mycorrhizal fungus from Australia, Cairneyella variabilis, Midgley and Tran-Dinh, gen. nov. sp. nov. The genome of C. variabilis was sequenced and a draft genome assembled. The draft genome of C. variabilis is 52.4 Mbp in length, and to our knowledge, this is the first study to present a genome of an ericoid mycorrhizal fungus from the southern hemisphere. Using the SignalP and dbCAN bioinformatic pipelines, a study of the catabolic potential of C. variabilis was undertaken and showed genes for an array of degradative enzymes, most of which appear to be secreted from the hyphae, to access a suite of different carbon sources. Isolates of C. variabilis have been previously shown to utilise cellulose, carboxymethyl cellulose (CMC), cellobiose, xylan, pectin, starch and tannic acid for growth, and in the current study, putative enzymes for these processes were revealed. These enzymes likely play key roles in nutrient cycling and other edaphic processes in heathland environments. ITS phylogenetic analyses showed C. variabilis to be distinct from the fungi of the “Hymenoscyphus ericae aggregate”.
KeywordsFungi Ericaceae Helotiales
We thank Mr Mark Wilson for accessioning cultures into the FRR culture collection, and Dr. Mark Bradbury and Ms. Brodie Sutcliffe for their insightful comments on the text.
- Atlas of Living Australia (2013) http://www.ala.org.au/.
- Bougoure DS, Cairney JWG (2005b) Fungi associated with hair roots of Rhododendron lochiae (Ericaceae) in an Australian tropical cloud forest revealed by culturing and culture-independent molecular methods. Environ Microbiol 7:1743–1754. doi: 10.1111/j.1462-2920.2005.00919.x CrossRefPubMedGoogle Scholar
- Curlevski NJ A, Chambers SM, Anderson IC, Cairney JWG (2009) Identical genotypes of an ericoid mycorrhiza-forming fungus occur in roots of Epacris pulchella (Ericaceae) and Leptospermum polygalifolium (Myrtaceae) in an Australian sclerophyll forest. FEMS Microbiol Ecol 67:411–420. doi: 10.1111/j.1574-6941.2008.00637.x CrossRefPubMedGoogle Scholar
- Dereeper A, Guignon V, Blanc G, Audic S, Buffet S, Chevenet F, Dufayard J-F, Guindon S, Lefort V, Lescot M, Claverie J-M, Gascuel O (2008) Phylogeny.fr: robust phylogenetic analysis for the non-specialist. Nucleic Acids Res 36:W465–W469. doi: 10.1093/nar/gkn180 CrossRefPubMedPubMedCentralGoogle Scholar
- Greenfield P, Duesing K, Papanicolaou A, Bauer DC (2014) Blue: correcting sequencing errors using consensus and context. Bioinformatics. 1–8. doi: 10.1093/bioinformatics/btu368
- Kohler A, Kuo A, Nagy LG, Morin E, Barry KW, Buscot F, Canbäck B, Choi C, Cichocki N, Clum A, Colpaert J, Copeland A, Costa MD, Doré J, Floudas D, Gay G, Girlanda M, Henrissat B, Herrmann S, Hess J, Högberg N, Johansson T, Khouja H-R, LaButti K, Lahrmann U, Levasseur A, Lindquist EA, Lipzen A, Marmeisse R et al (2015) Convergent losses of decay mechanisms and rapid turnover of symbiosis genes in mycorrhizal mutualists. Nat Genet 47:410–415. doi: 10.1038/ng.3223 CrossRefPubMedGoogle Scholar
- Palmer J, Horton B, Allaway W, Ashford A (2007) Growth stimulation of Woollsia pungens by a natural ericoid mycorrhizal fungal endophyte. Australas Mycol 26:1–8Google Scholar
- Williams AF, Chambers SM, Davies PW, Mclean CB, Cairney JWG (2004) Molecular investigation of sterile root-associated fungi from Epacris microphylla R. Br. (Ericaceae) and other epacrids at alpine, subalpine and coastal heathland sites. Australas Mycol 23:94–104Google Scholar