Mycorrhiza

, Volume 26, Issue 5, pp 389–399 | Cite as

Temporal changes of bacterial communities in the Tuber melanosporum ectomycorrhizosphere during ascocarp development

  • Aurélie Deveau
  • Sanjay Antony-Babu
  • François Le Tacon
  • Christophe Robin
  • Pascale Frey-Klett
  • Stéphane Uroz
Original Article

Abstract

Ectomycorrhizae create a multitrophic ecosystem formed by the association between tree roots, mycelium of the ectomycorrhizal fungus, and a complex microbiome. Despite their importance in the host tree’s physiology and in the functioning of the ectomycorrhizal symbiosis, detailed studies on ectomycorrhiza-associated bacterial community composition and their temporal dynamics are rare. Our objective was to investigate the composition and dynamics of Tuber melanosporum ectomycorrhiza-associated bacterial communities from summer to winter seasons in a Corylus avellana tree plantation. We used 16S ribosomal RNA (rRNA)-based pyrosequencing to compare the bacterial community structure and the richness in T. melanosporum’s ectomycorrhizae with those of the bulk soil. The T. melanosporum ectomycorrhizae harbored distinct bacterial communities from those of the bulk soil, with an enrichment in Alpha- and Gamma-proteobacteria. In contrast to the bacterial communities of truffle ascocarps that vastly varies in composition and richness during the maturation of the fruiting body and to those from the bulk soil, T. melanosporum ectomycorrhiza-associated bacterial community composition stayed rather stable from September to January. Our results fit with a recent finding from the same experimental site at the same period that a continuous supply of carbohydrates and nitrogen occurs from ectomycorrhizae to the fruiting bodies during the maturation of the ascocarps. We propose that this creates a stable niche in the ectomycorrhizosphere although the phenology of the tree changes.

Keywords

Tuber melanosporum Bacterial communities Temporal changes Soil-ectomycorrhizae interface Ectomycorrhizosphere 16S rRNA-based pyrosequencing 

Supplementary material

572_2015_679_MOESM1_ESM.docx (134 kb)
ESM 1(DOCX 133 kb)
572_2015_679_MOESM2_ESM.gif (154 kb)
ESM 2Figure S1. Multivariate analyses of the bacterial communities associated with T. melanosporum ectomycorrhizal root tip, bulk soil and ascocarps (peridium and gleba) in November (A), December (B) and January (C). MBS: bulk soil, MYCO: ectomycorrhizal root tip, AIN: gleba, AOUT: peridium. (GIF 154 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Aurélie Deveau
    • 1
    • 2
  • Sanjay Antony-Babu
    • 1
    • 2
    • 6
  • François Le Tacon
    • 1
    • 2
  • Christophe Robin
    • 4
    • 5
  • Pascale Frey-Klett
    • 1
    • 2
  • Stéphane Uroz
    • 1
    • 2
    • 3
  1. 1.UMR1136 INRA Université de Lorraine, Interactions Arbres – MicroorganismesChampenouxFrance
  2. 2.Université de Lorraine, UMR1136, Interactions Arbres – MicroorganismesVandœuvre-lès-NancyFrance
  3. 3.INRA, UR1138, Biogéochimie des écosystèmes forestiersChampenouxFrance
  4. 4.Université de Lorraine, UMR 1121, Agronomie & Environnement, Nancy-ColmarVandœuvre-lès-NancyFrance
  5. 5.INRA, UMR 1121, Agronomie & Environnement, Nancy-Colmar, Centre INRA de Nancy-LorraineVandœuvre-lès-NancyFrance
  6. 6.Department of Food Science and TechnologyUniversity of Nebraska-LincolnLincolnUSA

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