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Rhizophagus irregularis MUCL 41833 can colonize and improve P uptake of Plantago lanceolata after exposure to ionizing gamma radiation in root organ culture

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Abstract

Long-lived radionuclides such as 90Sr and 137Cs can be naturally or accidentally deposited in the upper soil layers where they emit β/γ radiation. Previous studies have shown that arbuscular mycorrhizal fungi (AMF) can accumulate and transfer radionuclides from soil to plant, but there have been no studies on the direct impact of ionizing radiation on AMF. In this study, root organ cultures of the AMF Rhizophagus irregularis MUCL 41833 were exposed to 15.37, 30.35, and 113.03 Gy gamma radiation from a 137Cs source. Exposed spores were subsequently inoculated to Plantago lanceolata seedlings in pots, and root colonization and P uptake evaluated. P. lanceolata seedlings inoculated with non-irradiated AMF spores or with spores irradiated with up to 30.35 Gy gamma radiation had similar levels of root colonization. Spores irradiated with 113.03 Gy gamma radiation failed to colonize P. lanceolata roots. P content of plants inoculated with non-irradiated spores or of plants inoculated with spores irradiated with up to 30.35 Gy gamma radiation was higher than in non-mycorrhizal plants or plants inoculated with spores irradiated with 113.03 Gy gamma radiation. These results demonstrate that spores of R. irregularis MUCL 41833 are tolerant to chronic ionizing radiation at high doses.

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Acknowledgment

DK received a mobility grant attributed by the Belgian Federal Science Policy and co-financed by the Marie Curie Actions PCOFUND-GA-2009-246540 of the European Commission.

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Authors and Affiliations

  1. Biosphere Impact Studies Unit, Belgian Nuclear Research Center (SCK•CEN), Boeretang 200, 2400, Mol, Belgium

    David Kothamasi, Jean Wannijn, May van Hees, Robin Nauts, Axel van Gompel, Nathalie Vanhoudt & Hildegarde Vandenhove

  2. Earth and Life Institute, Applied Microbiology, Mycology, Mycothèque de l’Université catholique de Louvain (MUCL), Université catholique de Louvain, Croix du Sud 2, bte L7.05.06, B-1348, Louvain-la-Neuve, Belgium

    Sylvie Cranenbrouck

  3. Earth and Life Institute, Applied Microbiology, Mycology, Université catholique de Louvain, Croix du Sud 2, bte L7.05.06, B-1348, Louvain-la-Neuve, Belgium

    Stéphane Declerck

  4. Laboratory of Soil Biology and Microbial Ecology, Department of Environmental Studies, University of Delhi, Delhi, 110 007, India

    David Kothamasi

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  1. David Kothamasi
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  2. Jean Wannijn
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  3. May van Hees
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  8. Stéphane Declerck
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  9. Hildegarde Vandenhove
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Correspondence to David Kothamasi.

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Kothamasi, D., Wannijn, J., van Hees, M. et al. Rhizophagus irregularis MUCL 41833 can colonize and improve P uptake of Plantago lanceolata after exposure to ionizing gamma radiation in root organ culture. Mycorrhiza 26, 257–262 (2016). https://doi.org/10.1007/s00572-015-0664-1

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