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Characterisation of seven Inocybe ectomycorrhizal morphotypes from a semiarid woody steppe

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Abstract

Ectomycorrhizas (ECM) of Inocybe species (Inocybaceae, Basidiomycota) formed by three host plant species (Populus alba, Salix rosmarinifolia and Pinus nigra) in a semiarid woody steppe of Hungary were studied. To identify the fungal partners, we performed phylogenetic analyses of nucleotide sequences for the internal transcribed spacer region of nuclear DNA (nrDNA ITS) together with sequences gained from public databases. Seven Inocybe ectomycorrhiza morphotypes were morpho-anatomically characterised. Five morphotypes were identified (I. phaeoleuca, I. psammophila, I. semifulva, I. splendens and I. subporospora), whereas two morphotypes represented unidentified Inocybe species. Differences were discernible among the morphotypes, and they showed general anatomical characteristics of Inocybe ECM, such as the slightly organised plectenchymatic mantle (types A, B and E and the gelatinous C). The ECM of I. subporospora and I. phaeoleuca were detected from the introduced Pinus nigra. These two fungi are probably native to the area but capable of forming a novel ectomycorrhizal association with the invasive host.

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Acknowledgments

We thank Csilla Jónás for preparing slides for microscopy and Márk Z. Németh for assistance with image processing. Gábor M. Kovács is supported by a Bolyai János Research Fellowship (Hungarian Academy of Sciences).

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Authors and Affiliations

  1. Institute of Biology, Department of Plant Anatomy, Eötvös Loránd University, Pázmány Péter sétány 1/c, H-1117, Budapest, Hungary

    Diána Seress, Bálint Dima & Gábor M. Kovács

  2. Centre for Agricultural Research, Hungarian Academy of Sciences, Plant Protection Institute, Herman Ottó street 15, H-1022, Budapest, Hungary

    Diána Seress

  3. Department of Biosciences, Plant Biology, University of Helsinki, P.O. Box 65, FI-00014, Helsinki, Finland

    Bálint Dima

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  1. Diána Seress
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  2. Bálint Dima
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Correspondence to Gábor M. Kovács.

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Seress, D., Dima, B. & Kovács, G.M. Characterisation of seven Inocybe ectomycorrhizal morphotypes from a semiarid woody steppe. Mycorrhiza 26, 215–225 (2016). https://doi.org/10.1007/s00572-015-0662-3

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