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The ectomycorrhizal community of conifer stands on peat soils 12 years after fertilization with wood ash

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Abstract

We studied long-term effects of fertilization with wood ash on biomass, vitality and mycorrhizal colonization of fine roots in three conifer forest stands growing in Vacciniosa turf. mel. (V), Myrtillosa turf. mel. (M) and Myrtillosa turf. mel./Caricoso-phragmitosa (MC) forest types on peat soils. Fertilization trials amounting 5 kg/m2 of wood ash were established 12 years prior to this study. A total of 63 soil samples with roots were collected and analysed. Ectomycorrhizal (ECM) fungi in roots were identified by morphotyping and sequencing of the fungal internal transcribed spacer (ITS) region. In all forest types, fine root biomass was higher in fertilized plots than in control plots. In M forest type, proportion of living fine roots was greater in fertilized plots than in control plots, while in V and MC, the result was opposite. Fifty ECM species were identified, of which eight were common to both fertilized and control plots. Species richness and Shannon diversity index were generally higher in fertilized plots than in control plots. The most common species in fertilized plots were Amphinema byssoides (17.8 %) and Tuber cf. anniae (12.2 %), while in control plots, it was Tylospora asterophora (18.5 %) and Lactarius tabidus (20.3 %). Our results showed that forest fertilization with wood ash has long-lasting effect on diversity and composition of ECM fungal communities.

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Acknowledgments

The research was funded by the ERDF project No. 2013/0065/2DP/2.1.1.1.0/13/APIA/VIAA/034. We are grateful to the Natural Resources Institute Finland (former Finnish Forest Research Institute) for the laboratory facilities and the support in the laboratory work. Our special thanks to colleagues from LSFRI “Silava” who established this experiment and were involved in monitoring and sampling (A. Indriksons, P. Zālītis, A. Brūvelis, A. Lupiķis and others).

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Authors and Affiliations

  1. Latvian State Forest Research Institute “Silava”, Rigas 111, Salaspils, LV-2169, Latvia

    Darta Klavina, Talis Gaitnieks, Andis Lazdins & Dagnija Lazdina

  2. Natural Resources Institute Finland (Luke), Vantaa Research Centre, Jokiniemenkuja 1, FI-01370, Vantaa, Finland

    Taina Pennanen & Sannakajsa Velmala

  3. Department of Forest Mycology and Plant Pathology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-75007, Uppsala, Sweden

    Audrius Menkis

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  1. Darta Klavina
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  2. Taina Pennanen
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Correspondence to Darta Klavina.

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Klavina, D., Pennanen, T., Gaitnieks, T. et al. The ectomycorrhizal community of conifer stands on peat soils 12 years after fertilization with wood ash. Mycorrhiza 26, 153–160 (2016). https://doi.org/10.1007/s00572-015-0655-2

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