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Using mycorrhiza-defective mutant genotypes of non-legume plant species to study the formation and functioning of arbuscular mycorrhiza: a review

Abstract

A significant challenge facing the study of arbuscular mycorrhiza is the establishment of suitable non-mycorrhizal treatments that can be compared with mycorrhizal treatments. A number of options are available, including soil disinfection or sterilisation, comparison of constitutively mycorrhizal and non-mycorrhizal plant species, comparison of plants grown in soils with different inoculum potential and the comparison of mycorrhiza-defective mutant genotypes with their mycorrhizal wild-type progenitors. Each option has its inherent advantages and limitations. Here, the potential to use mycorrhiza-defective mutant and wild-type genotype plant pairs as tools to study the functioning of mycorrhiza is reviewed. The emphasis of this review is placed on non-legume plant species, as mycorrhiza-defective plant genotypes in legumes have recently been extensively reviewed. It is concluded that non-legume mycorrhiza-defective mutant and wild-type pairs are useful tools in the study of mycorrhiza. However, the mutant genotypes should be well characterised and, ideally, meet a number of key criteria. The generation of more mycorrhiza-defective mutant genotypes in agronomically important plant species would be of benefit, as would be more research using these genotype pairs, especially under field conditions.

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Acknowledgments

We wish to thank Prof. Sally Smith for comments on a very early draft of the manuscript. We also gratefully acknowledge Prof. Sally Smith and A/Prof. Susan Barker for continued access to the rmc and 76R genotypes of tomato. TRC also thanks Prof. Louise Jackson and members of her group for many valuable discussions over the years. TRC also wishes to acknowledge the Australian Research Council for financial support (FT120100463). SJWW wishes to acknowledge support received from the Monash University Postgraduate Publications Award.

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Watts-Williams, S.J., Cavagnaro, T.R. Using mycorrhiza-defective mutant genotypes of non-legume plant species to study the formation and functioning of arbuscular mycorrhiza: a review. Mycorrhiza 25, 587–597 (2015). https://doi.org/10.1007/s00572-015-0639-2

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Keywords

  • Arbuscular mycorrhiza
  • Mycorrhiza-defective mutant genotype
  • Reduced mycorrhizal colonisation (rmc)
  • Solanum lycopersicum (tomato)
  • Micro-Tom