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The arbuscular mycorrhizal symbiosis attenuates symptom severity and reduces virus concentration in tomato infected by Tomato yellow leaf curl Sardinia virus (TYLCSV)

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Abstract

The arbuscular mycorrhizal (AM) symbiosis is considered a natural instrument to improve plant health and productivity since mycorrhizal plants often show higher tolerance to abiotic and biotic stresses. However, the impact of the AM symbiosis on infection by viral pathogens is still largely uncertain and little explored. In the present study, tomato plants were grown under controlled conditions and inoculated with the AM fungus Funneliformis mosseae. Once the mycorrhizal colonization had developed, plants were inoculated with the Tomato yellow leaf curl Sardinia virus (TYLCSV), a geminivirus causing one of the most serious viral diseases of tomatoes in Mediterranean areas. Biological conditions consisted of control plants (C), TYLCSV-infected plants (V), mycorrhizal plants (M), and TYLCSV-infected mycorrhizal plants (MV). At the time of analysis, the level of mycorrhiza development and the expression profiles of mycorrhiza-responsive selected genes were not significantly modified by virus infection, thus indicating that the AM symbiosis was unaffected by the presence and spread of the virus. Viral symptoms were milder, and both shoot and root concentrations of viral DNA were lower in MV plants than in V plants. Overall F. mosseae colonization appears to exert a beneficial effect on tomato plants in attenuating the disease caused by TYLCSV.

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Acknowledgments

We thank Fondazione CRT for funding in part the PhD fellowship of GM. This work was supported by the CISIA Project (CNR, Italy) and by the “GenoPom” project (MIUR, Italy) to GPA and by the “60 % University grant” to LL. We are grateful to M. Vecchiati for technical assistance.

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Authors and Affiliations

  1. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Viale Mattioli 25, 10125, Torino, Italy

    Giulia Maffei, Valentina Fiorilli, Mara Novero & Luisa Lanfranco

  2. Istituto di Virologia Vegetale, Consiglio Nazionale delle Ricerche, Strada delle Cacce 73, 10135, Torino, Italy

    Giulia Maffei, Laura Miozzi & Gian Paolo Accotto

  3. Istituto per la Protezione delle Piante, Consiglio Nazionale delle Ricerche, Viale Mattioli 25, 10125, Torino, Italy

    Valentina Fiorilli & Luisa Lanfranco

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  1. Giulia Maffei
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  2. Laura Miozzi
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  3. Valentina Fiorilli
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  4. Mara Novero
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  5. Luisa Lanfranco
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  6. Gian Paolo Accotto
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Correspondence to Gian Paolo Accotto.

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Maffei, G., Miozzi, L., Fiorilli, V. et al. The arbuscular mycorrhizal symbiosis attenuates symptom severity and reduces virus concentration in tomato infected by Tomato yellow leaf curl Sardinia virus (TYLCSV). Mycorrhiza 24, 179–186 (2014). https://doi.org/10.1007/s00572-013-0527-6

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  • DOI: https://doi.org/10.1007/s00572-013-0527-6

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