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Little to no genetic structure in the ectomycorrhizal basidiomycete Suillus spraguei (Syn. S. pictus) across parts of the northeastern USA

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Abstract

Population genetic studies of ectomycorrhizal fungi at scales larger than 100 km are still relatively rare with highly variable results. In this study, we determined the population genetic structure of the ectomycorrhizal basidiomycete Suillus spraguei over distances up to 600 km in northeastern USA forests. S. spraguei associates exclusively with five-needled pines and only with white pine (Pinus strobus) in the eastern USA. We used six microsatellite loci to assess the genetic structure between eight sites sampled in the Adirondack Park of New York and seven sites sampled in other forests of New York, Pennsylvania, and Massachusetts. Except for one site, little to no genetic differentiation was detected in pairwise comparisons of the sites (F ST = 0 to 0.05). Only one site was moderately differentiated from most other sites (F ST = 0.02 to 0.15). The Mantel test showed no significant correlation between genetic and geographic distances (isolation by distance; R 2 = 0.003, P = 0.3). The STRUCTURE analysis also supported the presence of a single cluster (K = 1).

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References

  • Abrams MD (2001) Eastern white pine versatility in the presettlement forest. BioScience 51(11):967–978

    Article  Google Scholar 

  • Amend A, Garbelotto M, Fang Z, Keeley S (2010) Isolation by landscape in populations of a prized edible mushroom Tricholoma matsutake. Conserv Genet 11(3):795–802

    Article  Google Scholar 

  • Ashkannejhad S, Horton TR (2006) Ectomycorrhizal ecology under primary succession on coastal sand dunes: interactions involving Pinus contorta, suilloid fungi and deer. New Phytol 169(2):345–354

    Article  PubMed  Google Scholar 

  • Bergemann SE, Miller SL (2002) Size, distribution, and persistence of genets in local populations of the late-stage ectomycorrhizal basidiomycete, Russula brevipes. New Phytol 156:313–320

    Article  CAS  Google Scholar 

  • Bergemann SE, Douhan GW, Garbelotto M, Miller SL (2006) No evidence of population structure across three isolated subpopulations of Russula brevipes in an oak/pine woodland. New Phytol 170:177–184

    Article  PubMed  Google Scholar 

  • Burchhardt KM, Rivera Y, Baldwin T, VanEarden D, Kretzer A (2011) Analysis of genet size and local gene flow in the ectomycorrhizal basidiomycete Suillus spraguei (synonym S. pictus). Mycologia 103(4):722–730

    Article  CAS  PubMed  Google Scholar 

  • Carriconde F, Gardes M, Jargeat P, Heilmann-Clausen J, Mouhamadou B, Gryta H (2008a) Population evidence of cryptic species and geographical structure in the cosmopolitan ectomycorrhizal fungus, Tricholoma scalpturatum. Microb Ecol 56(3):513–524

    Article  PubMed  Google Scholar 

  • Carriconde F, Gryta H, Jargeat P, Mouhamadou B, Gardes M (2008b) High sexual reproduction and limited contemporary dispersal in the ectomycorrhizal fungus Tricholoma scalpturatum: new insights from population genetics and spatial autocorrelation analysis. Mol Ecol 17(20):4433–4445

    Article  CAS  PubMed  Google Scholar 

  • Douhan GW, Vincenot L, Gryta H, Selosse MA (2011) Population genetics of ectomycorrhizal fungi: from current knowledge to emerging directions. Fungal Biol 115:569–597

    Article  PubMed  Google Scholar 

  • Dunham S, O'Dell TE, Molina R (2006) Spatial analysis of within-population microsatellite variability reveals restricted gene flow in the Pacific golden chanterelle (Cantharellus formosus). Mycologia 98(2):250–259

    Article  CAS  PubMed  Google Scholar 

  • Dunham SM, Mujic AB, Spatafora JW, Kretzer AM (2013) Within-population genetic structure differs between two sympatric sister-species of ectomycorrhizal fungi, Rhizopogon vinicolor and R. vesiculosus. Mycologia 105(4):814–826

    Article  PubMed  Google Scholar 

  • Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620

    Article  CAS  PubMed  Google Scholar 

  • Excoffier L, Smouse P, Quattro J (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    CAS  PubMed Central  PubMed  Google Scholar 

  • Foster DR, Motzkin G, Slater B (1998) Land-use history as long-term broad-scale disturbance: regional forest dynamics in central New England. Ecosystems 1:96–119

    Article  Google Scholar 

  • Glitzenstein J, Canham C, McDonnell M, Streng D (1990) Interactions between land-use history and environment in upland forests of the Cary Arboretum, Hudson Valley, New York. B Torrey Bot Club 117:106–122

    Article  Google Scholar 

  • Goudet J (2002) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3.2). http://www2.unil.ch/popgen/softwares/fstat.htm. Accessed 15 Mar 2013

  • Goudet J, Raymond M, de-Meeus T, Rousset F (1996) Testing differentiation in diploid populations. Genetics 144:1933–1940

    CAS  PubMed Central  PubMed  Google Scholar 

  • Grubisha LC, Bergemann SE, Bruns TD (2007) Host islands within the California Northern Channel Islands create fine-scale genetic structure in two sympatric species of the symbiotic ectomycorrhizal fungus Rhizopogon. Mol Ecol 16:1811–1822

    Article  PubMed  Google Scholar 

  • Hale ML, Burg TM, Steeves TE (2012) Sampling for microsatellite-based population genetic studies: 25 to 30 individuals per population is enough to accurately estimate allele frequencies. PLoS ONE 7:e45170

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hirose D, Kikuchi J, Kanzaki N, Futai K (2004) Genet distribution of sporocarps and ectomycorrhizas of Suillus pictus in a Japanese white pine plantation. New Phytol 164:527–541

    Article  Google Scholar 

  • Johnson CN (1996) Interactions between mammals and ectomycorrhizal fungi. Trends Ecol Evol 11(12):503–507

    Article  CAS  PubMed  Google Scholar 

  • Kretzer AM, Dunham S, Molina R, Spatafora JW (2005) Patterns of vegetative growth and gene flow in Rhizopogon vinicolor and R. vesiculosus (Boletales, Basidiomycota). Mol Ecol 14:2259–2268

    Article  CAS  PubMed  Google Scholar 

  • Landguth EL, Fedy BC, Oyler-McCance SJ, Garey AL, Emel SL, Mumma M, Wagner HH, Fortin MJ, Cushman SA (2012) Effects of sample size, number of markers, and allelic richness on the detection of spatial genetic pattern. Mol Ecol Resour 12:276–284

    Article  Google Scholar 

  • Ledig FT (1992) Human impacts on genetic diversity in forest ecosystems. Oikos 63:87–108

    Article  Google Scholar 

  • Muller LAH, Vangronsveld J, Colpaert JV (2007) Genetic structure of Suillus luteus populations in heavy metal polluted and non-polluted habitats. Mol Ecol 16(22):4728–4737

    Article  CAS  PubMed  Google Scholar 

  • Murat C, Díez J, Luis P, Delaruelle C, Dupré C, Chevalier G, Bonfante P, Martin F (2004) Polymorphism at the ribosomal DNA ITS and its relation to postglacial re-colonization routes of the Perigord truffle Tuber melanosporum. New Phytol 164:401–411

    Article  CAS  Google Scholar 

  • Niering WA (1998) Forces that shaped the forests of the Northeastern United States. Northeast Nat 5(2):99–110

    Article  Google Scholar 

  • Peakall R, Smouse P (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Pritchard J, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959

    CAS  PubMed Central  PubMed  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Rousset F (2008) Genepop'007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Roy M, Dubois MP, Proffit M, Vincenot L, Desmarais E, Selosse MA (2008) Evidence from population genetics that the ectomycorrhizal basidiomycete Laccaria amethystina is an actual multihost symbiont. Mol Ecol 17(12):2825–2838

    Article  CAS  PubMed  Google Scholar 

  • Rubini A, Paolocci F, Riccioni C, Vendramin GG, Arcioni S (2005) Genetic and phylogeographic structures of the symbiotic fungus Tuber magnatum. Appl Environ Microbiol 71(11):6584–6589

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Smith BE, Marks PL, Gardescu S (1993) Two hundred years of forest cover changes in Tompkins County, New York. B Torrey Bot Club 120(3):229–247

    Article  Google Scholar 

  • Vincenot L, Nara K, Sthultz C, Labbe J, Dubois MP, Tedersoo L, Martin F, Selosse MA (2012) Extensive gene flow over Europe and possible speciation over Eurasia in the ectomycorrhizal basidiomycete Laccaria amethystina complex. Mol Ecol 21(2):281–299

    Article  CAS  PubMed  Google Scholar 

  • Whitney GG, Davis W (1986) Thoreau and the forest history of Concord, Massachusetts. J For Hist 30:70–81

    Google Scholar 

  • Wright S (1969) Evolution and the genetics of populations, Vol 2. The theory of gene frequencies. University of Chicago Press, Chicago

    Google Scholar 

  • Xu J, Sha T, Li YC, Zhao ZW, Yang ZL (2008) Recombination and genetic differentiation among natural populations of the ectomycorrhizal mushroom Tricholoma matsutake from southwestern China. Mol Ecol 17(5):1238–1247

    Article  PubMed  Google Scholar 

  • Zhou Z, Miwa M, Hogetsu T (2001) Polymorphism of simple sequence repeats reveals gene flow within and between ectomycorrhizal Suillus grevillei populations. New Phytol 149(2):339–348

    Article  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank Tera Galante, Jed Cappellazzi, David VanEarden, Joel Horman, Dr. Manfred Binder, Dr. Thomas Horton, and the Central New York Mycological Society for their help collecting basidiocarps of S. spraguei. Dr. Sarah Bergemann and two anonymous reviewers have made helpful comments on an earlier version of this manuscript. Funding for this research was provided by a USDA–CSREES McIntire–Stennis Grant (to Annette Kretzer).

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Authors and Affiliations

  1. Department of Environmental and Forest Biology, College of Environmental Science and Forestry, State University of New York, 1 Forestry Drive, Syracuse, NY, 13210, USA

    Yazmín Rivera & Annette M. Kretzer

  2. Department of Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA

    Kathleen M. Burchhardt

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  1. Yazmín Rivera
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  2. Kathleen M. Burchhardt
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  3. Annette M. Kretzer
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Correspondence to Yazmín Rivera.

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Rivera, Y., Burchhardt, K.M. & Kretzer, A.M. Little to no genetic structure in the ectomycorrhizal basidiomycete Suillus spraguei (Syn. S. pictus) across parts of the northeastern USA. Mycorrhiza 24, 227–232 (2014). https://doi.org/10.1007/s00572-013-0524-9

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