, Volume 24, Issue 1, pp 35–43 | Cite as

Symbiotic seed germination and protocorm development of Aa achalensis Schltr., a terrestrial orchid endemic from Argentina

  • Fracchia Sebastián
  • Silvani Vanesa
  • Flachsland Eduardo
  • Terada Graciela
  • Sede Silvana
Original Paper


Aa achalensis is an endangered terrestrial orchid endemic from Argentina. In vitro symbiotic seed germination was evaluated for its propagation. Five different fungal strains were isolated from this species: two Rhizoctonia-like related to Thanatephorus cucumeris and three ascomicetaceous fungi belonging to Phialophora graminicola and one to an uncultured Pezizaceae. All five isolates promoted seed germination being one T. cucumeris strain the most effective. After 16 weeks of growth, 30 % of A. achalensis protocorms developed until seedlings with two/four leaves in this treatment. These findings open an opportunity to the knowledge and preservation of this species.


Orchid mycorrhiza Seed germination Dark septate endophytes Conservation 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Fracchia Sebastián
    • 1
  • Silvani Vanesa
    • 2
  • Flachsland Eduardo
    • 3
  • Terada Graciela
    • 3
  • Sede Silvana
    • 4
  1. 1.Centro Regional de Investigaciones Científicas y Transferencia Tecnológica, CRILAR- CONICETAnillacoArgentina
  2. 2.Departamento de Biodiversidad y Biología Experimental. Facultad de Ciencias Exactas y Naturales (FCEyN)Universidad de Buenos Aires (UBA)Buenos AiresArgentina
  3. 3.Instituto de Botánica del Nordeste (CONICET—Consejo Nacional de Investigaciones Científicas y Técnicas)Facultad de Ciencias Agrarias (UNNE—Universidad Nacional del Nordeste)CorrientesArgentina
  4. 4.Instituto de Botánica DarwinionBuenos AiresArgentina

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