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Association of Pinus banksiana Lamb. and Populus tremuloides Michx. seedling fine roots with Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina)

Mycorrhiza volume 22, pages 631–638 (2012)Cite this article

Abstract

Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina, Hydanaceae), commonly regarded as a wood decay fungus, was consistently isolated from bareroot nursery Pinus banksiana Lamb. seedlings. S. brinkmannii was found in ectomycorrhizae formed by Thelephora terrestris Ehrh., Laccaria laccata (Scop.) Cooke, and Suillus luteus (L.) Roussel. In pure culture combinations with sterile P. banksiana and Populus tremuloides Michx. seedlings, S. brinkmannii colonized root cortical cells while not killing seedlings. Colonization by S. brinkmannii appeared to be intracellular but typical endo- or ectomycorrhizae were not formed. The fungus did not decay roots, although it was shown to produce cellulase in enzyme tests. Results suggest a unique association between S. brinkmannii and seedling roots that is neither mycorrhizal nor detrimental; its exact function remains to be elucidated.

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Acknowledgments

We thank Dr. Erik Lilleskov who provided substantial assistance with phylogenetic analysis; Dr. Harold H. Burdsall Jr. who provided valuable comments on the early taxonomy of S. brinkmannii; Drs. Linda van Diepen and Carrie Andrew for assistance with staining and molecular methodology; and Karena Schmidt for assistance with figures. We also thank the anonymous reviewers for their role in shaping our manuscript. This work was supported in part by the USDA Forest Service J.W. Toumey Nursery, the National Center for Reforestation, Nurseries, and Genetics Resources, the Rocky Mountain Research Station, and Michigan Technological University.

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Authors and Affiliations

  1. USDA Forest Service, Northern Research Station, Houghton, MI, 49931, USA

    Lynette R. Potvin

  2. School of Forest Resources & Environmental Science, Michigan Technological University, 1400 Townsend Drive, Houghton, MI, 49931, USA

    Dana L. Richter & Martin F. Jurgensen

  3. USDA Forest Service, Rocky Mountain Research Station, 1221 South Main St., Moscow, ID, 83843, USA

    R. Kasten Dumroese

Authors
  1. Lynette R. Potvin
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  2. Dana L. Richter
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  3. Martin F. Jurgensen
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  4. R. Kasten Dumroese
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Corresponding author

Correspondence to Lynette R. Potvin.

Appendix

Appendix

Table 1 ITS sequences in the GenBank database used for Bayesian phylogenetic analysis. Query coverage, No. of bp compared, and ITS similarity are in relation to the sequence of interest for this study, which is indicated in bold
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Potvin, L.R., Richter, D.L., Jurgensen, M.F. et al. Association of Pinus banksiana Lamb. and Populus tremuloides Michx. seedling fine roots with Sistotrema brinkmannii (Bres.) J. Erikss. (Basidiomycotina). Mycorrhiza 22, 631–638 (2012). https://doi.org/10.1007/s00572-012-0440-4

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Keywords

  • Sistotrema brinkmannii
  • Pinus banksiana
  • Populus tremuloides

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