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The differential behavior of arbuscular mycorrhizal fungi in interaction with Astragalus sinicus L. under salt stress

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Abstract

Three arbuscular mycorrhizal (AM) fungi (Glomus mosseae, Glomus claroideum, and Glomus intraradices) were compared for their root colonizing ability and activity in the root of Astragalus sinicus L. under salt-stressed soil conditions. Mycorrhizal formation, activity of fungal succinate dehydrogenase, and alkaline phosphatase, as well as plant biomass, were evaluated after 7 weeks of plant growth. Increasing the concentration of NaCl in soil generally decreased the dry weight of shoots and roots. Inoculation with AM fungi significantly alleviated inhibitory effect of salt stress. G. intraradices was the most efficient AM fungus compared with the other two fungi in terms of root colonization and enzyme activity. Nested PCR revealed that in root system of plants inoculated with a mix of the three AM fungi and grown under salt stress, the majority of mycorrhizal root fragments were colonized by one or two AM fungi, and some roots were colonized by all the three. Compared to inoculation alone, the frequency of G. mosseae in roots increased in the presence of the other two fungal species and highest level of NaCl, suggesting a synergistic interaction between these fungi under salt stress.

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Abbreviations

ALP:

Alkaline phosphatase

AM:

Arbuscular mycorrhizal

LSU rDNA:

Large subunit ribosomal DNA

PCR:

Polymerase chain reaction

SDH:

Succinate dehydrogenase

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Acknowledgments

The authors are grateful to Prof. Lyn. Abbott (UWA, Australia) for the invaluable advice and to Dr. V. Gianinazzi-Pearson (INRA, Dijon, France) for the critical review of this manuscript. This study was partly supported by the National Natural Science Foundation of China (grant no. 30270051 and grant no. 4004-084011).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China

    Jin Peng, Yan Li, Ping Shi, Hui Lin & Bin Zhao

  2. Key Laboratory of Subtropical Agriculture & Environment, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, 430070, China

    Xiuhua Chen

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  1. Jin Peng
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  2. Yan Li
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  3. Ping Shi
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  4. Xiuhua Chen
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  5. Hui Lin
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  6. Bin Zhao
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Correspondence to Bin Zhao.

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Peng, J., Li, Y., Shi, P. et al. The differential behavior of arbuscular mycorrhizal fungi in interaction with Astragalus sinicus L. under salt stress. Mycorrhiza 21, 27–33 (2011). https://doi.org/10.1007/s00572-010-0311-9

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  • DOI: https://doi.org/10.1007/s00572-010-0311-9

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