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Position of the reduced mycorrhizal colonisation (Rmc) locus on the tomato genome map

Mycorrhiza volume 17, pages 311–318 (2007)Cite this article

Abstract

Our research aims to investigate the molecular communication between land plants and arbuscular mycorrhizal (AM) fungi in the establishment of symbiosis. We have identified a mutation in the facultative AM host tomato, which we named rmc. Plants that are homozygous for rmc no longer host most AM fungi. The mutation also affects the interaction of tomato with root knot nematode and Fusarium wilt. However, the function/s encoded by the intact Rmc locus is/are unknown. To clone and sequence the gene or genes that comprise the Rmc locus, we have initiated a positional cloning project. In this paper, we report the construction of mapping populations and use of molecular markers from the published genome map to identify the location of Rmc on tomato chromosome 8. Nucleotide binding site-leucine rich repeat resistance genes, reported to reside in the same region of that chromosome, provided insufficient differences to develop cleaved amplified polymorphic sequence markers. Therefore, we were unable to map these sequences in relation to rmc. Our results potentiate future work to identify the Rmc function and to determine the genetic basis for the multiple plant-microbe interaction functions that the rmc mutation has defined.

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Acknowledgement

This work was funded by Australian Research Council Large Grant A19943108, Australian Research Council Discovery Grant DP 0342496 and the School of Plant Biology UWA. We thank Professor LK Abbott (UWA) for provision of some biological materials and access to laboratory facilities, Ms T Edmonds-Tibbett for assistance with mycorrhizal screening techniques and Dr M Cakir for helpful discussions.

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  1. Nicholas J. Larkan

    Present address: Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, S7N0X2, Canada

Affiliations

  1. School of Plant Biology M084, Centre for Soil–Plant Interactions, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia

    Nicholas J. Larkan & Susan J. Barker

  2. Soil and Land Systems, School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, SA, 5005, Australia

    Sally E. Smith

  3. Centre for Soil–Plant Interactions, Perth, Australia

    Nicholas J. Larkan, Sally E. Smith & Susan J. Barker

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  1. Nicholas J. Larkan
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  2. Sally E. Smith
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Correspondence to Susan J. Barker.

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Larkan, N.J., Smith, S.E. & Barker, S.J. Position of the reduced mycorrhizal colonisation (Rmc) locus on the tomato genome map. Mycorrhiza 17, 311–318 (2007). https://doi.org/10.1007/s00572-007-0106-9

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  • DOI: https://doi.org/10.1007/s00572-007-0106-9

Keywords

  • Arbuscular mycorrhiza
  • Mutant
  • Bulked segregant analysis
  • Solanum lycopersicum
  • Microsatellite
  • RFLP
  • NBS-LRR R-genes