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Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius

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Abstract

Evidence for the participation of reactive oxygen species (ROS) and antioxidant systems in ectomycorrhizal (ECM) establishment is lacking. In this paper, we evaluated ROS production and the activities of superoxide dismutase (SOD) and catalase (CAT) during the early contact of the ECM fungus Pisolithus tinctorius with the roots of Castanea sativa (chestnut tree). Roots were placed in contact with P. tinctorius mycelia, and ROS production was evaluated by determining the levels of H2O2 and O2 ·− during the early stages of fungal contact. Three peaks of H2O2 production were detected, the first two coinciding with O2 ·− bursts. The first H2O2 production peak coincided with an increase in SOD activity, whereas CAT activity seemed to be implicated in H2O2 scavenging. P. tinctorius growth was evaluated in the presence of P. tinctorius-elicited C. sativa crude extracts prepared during the early stages of fungal contact. Differential hyphal growth that matched the H2O2 production profile with a delay was detected. The result suggests that during the early stages of ECM establishment, H2O2 results from an inhibition of ROS-scavenging enzymes and plays a role in signalling during symbiotic establishment.

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Acknowledgement

This work was supported by FCT (POCTI/BSE/38059/2001) and AGRO (project 689). P. Baptista was supported by the Portuguese Education Ministry (PRODEP fellowship).

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Authors and Affiliations

  1. CIMO/ESAB, Escola Superior Agrária de Bragança, Quinta de Sta Apolónia, Apartado 1172, 5301-855, Bragança, Portugal

    Paula Baptista & Anabela Martins

  2. Unidade de Biologia Molecular e Biotecnologia Vegetal, Instituto de Ciência Aplicada e Tecnologia, Edifício ICAT, Campo Grande, 1749-016, Lisboa, Portugal

    Maria Salomé Pais

  3. Departamento de Biologia, Laboratório de Bioquímica e Fisiologia Molecular de Plantas, Centro de Biologia, Universidade do Minho, Campus de Gualtar, 4710-057, Braga, Portugal

    Rui M. Tavares & Teresa Lino-Neto

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  1. Paula Baptista
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  2. Anabela Martins
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  3. Maria Salomé Pais
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  4. Rui M. Tavares
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  5. Teresa Lino-Neto
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Correspondence to Paula Baptista.

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Baptista, P., Martins, A., Pais, M.S. et al. Involvement of reactive oxygen species during early stages of ectomycorrhiza establishment between Castanea sativa and Pisolithus tinctorius . Mycorrhiza 17, 185–193 (2007). https://doi.org/10.1007/s00572-006-0091-4

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