Skip to main content
SpringerLink
Log in

Host-related variability in arbuscular mycorrhizal fungal structures in roots of Hedera rhombea, Rubus parvifolius, and Rosa multiflora under controlled conditions

  • Original Paper
  • Published:
Mycorrhiza Aims and scope Submit manuscript

Abstract

The arbuscular mycorrhizal (AM) morphology of three host plant species inoculated with single and mixed fungal culture and the distribution of AM fungal species in roots of the hosts treated with a mixed culture of AM fungi were determined. The aim was to investigate the effect of host plants and AM fungi on AM morphology of coexisting plant species. Noncolonized rooted cuttings of Hedera rhombea (Miq) Bean, Rubus parvifolius L., and Rosa multiflora Thunb. were inoculated with five fungal species as single and mixed culture inocula. The fungal species used were Gigaspora rosea and Scutellospora erythropa, previously isolated from H. rhombea; Acaulospora longula and Glomus etunicatum from R. parvifolius; and Glomus claroideum from both plant species. A few hyphal and arbusculate coils were seen in the mixed culture-inoculated roots of R. parvifolius; all fungal treatments produced this Paris-type AM in H. rhombea and Arum-type AM in R. parvifolius, and R. multiflora indicates that AM morphology is strongly controlled by the identity of the host plants used in this study. AM fungal rDNA was extracted separately from roots of each replicate plant species inoculated with the mixed fungal culture, amplified, cloned, sequenced, and analyzed to determine the AM fungal species and their respective proportions in roots of each plant species. Glomus etunicatum and G. claroideum of the family Glomaceae generally occurred more frequently in R. parvifolius and R. multiflora, which form Arum-types, whereas S. erythropa, of the family Gigasporaceae, was the most frequently detected species in H. rhombea, which produced Paris-type AM. Although the genotype of the plant species used appears to determine the AM morphologies formed, there was preferential association between the hosts and AM fungal inoculants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Adjoud D, Plenchette C, Halli-Hargas R, Lapeyrie F (1996) Response of 11 eucalyptus species to inoculation with three arbuscular mycorrhizal fungi. Mycorrhiza 6:129–135

    Article  Google Scholar 

  • Ahulu EM, Nakata M, Nonaka M (2005) Arum- and Paris-type arbuscular mycorrhizas in a mixed pine forest on sand dune soil in Niigata Prefecture, central Honshu, Japan. Mycorrhiza 15:129–136

    Article  Google Scholar 

  • Ahulu EM, Gollotte A, Gianinazzi-Pearson V, Nonaka M (2006) Co-occurring plants forming distinct arbuscular mycorrhizal morphologies harbour similar AM fungal species. Mycorrhiza (in press)

  • Bedini S, Maremmani A, Giovannetti M (2000) Paris-type mycorrhizas in Smilax aspera L. growing in a Mediterranean sclerophyllous wood. Mycorrhiza 10(1):9–13

    Article  Google Scholar 

  • Bever JD, Morton JB, Antonovics A, Schultz PA (1996) Host-dependent sporulation and species diversity of arbuscular mycorrhizal fungi in mown grassland. J Ecol 84:71–82

    Article  Google Scholar 

  • Bidartondo MI, Redecker D, Hijri I, Wiemken A, Bruns TD, Dominguez L, Sercic A, Leake JR, Read DJ (2002) Epiparasitic plants specialized on arbuscular mycorrhizal fungi. Nature 419:389–392

    Article  CAS  PubMed  Google Scholar 

  • Bonfante-Fasolo P, Fontana A (1985) VAM fungi in Ginkgo biloba roots: their interactions at the cellular level. Symbiosis 1:53–67

    Google Scholar 

  • Brundrett MC, Kendrick WB (1990a) The roots and mycorrhizae of herbaceous woodland plants. I. Quantitative aspects of morphology. New Phytol 114:457–468

    Article  Google Scholar 

  • Brundrett MC, Kendrick WB (1990b) The roots and mycorrhizae of herbaceous woodland plants. II. Structural aspects of morphology. New Phytol 114:469–479

    Article  Google Scholar 

  • Cavagnaro TR, Gao L-L, Smith FA, Smith SE (2001) Morphology of arbuscular mycorrhiza is influenced by fungal identity. New Phytol 151:469–475

    Article  Google Scholar 

  • Clapp JP, Young JPW, Merryweather JW, Fitter AH (1995) Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community. New Phytol 130:259–265

    Article  Google Scholar 

  • Dickson S (2004) The Arum–Paris continuum of mycorrhizal symbioses. New Phytol 1631:187–200

    Article  Google Scholar 

  • Eom AH, Hartnet DC, Wilson GWT (2000) Host plant species effects on arbuscular mycorrhizal fungal communities in tallgrass prairie. Oecologia 122:435–444

    Article  PubMed  Google Scholar 

  • Gollotte A, van Tuinen D, Atkinson D (2004) Diversity of mycorrhizal fungi colonizing roots of grass species Agrostis capillaries and Lolium perenne in a field experiment. Mycorrhiza 14:111–117

    Article  PubMed  Google Scholar 

  • Hart MM, Richard RJ (2002) Taxonomic basis for variation in the colonization strategy of arbuscular mycorrhizal fungi. New Phytol 153(2):335–344

    Article  Google Scholar 

  • Helgason T, Fitter AH, Young JPW (1999) Molecular diversity of arbuscular mycorhhizal fungi colonizing Hyacinthoides non-scripta (bluebell) in seminatural woodland. Mol Ecol 8:659–666

    Article  CAS  Google Scholar 

  • Imhof S, Weber HC (1997) Root anatomy and mycotrophy (AM) of the achlorophyllous Voyria truncata (Stanley) Stanley and Steyermark (Gentianaceae). Bot Acta 110:127–134

    Article  Google Scholar 

  • Imhof S, Weber HC (2000) Root structures and mycorrhiza of the achlorophyllous Voyria obconica progel (Gentianaceae). Symbiosis 29:201–211

    Google Scholar 

  • Jacquelinet-Jeanmougin S, Gianinazzi-Pearson V (1983) Endomycorrhizas in Gentianaceae. I. The fungus associated with Gentiana lutea L. New Phytol 95:663–666

    Article  Google Scholar 

  • Johnson D, Vandenkoornhuyse P, Leake JR, Gilbert L, Booth RE, Grime JP, Young JPW, Read DJ (2003) Plant communities affect arbuscular mycorrhizal fungal diversity and community composition in grassland microcosms. New Phytol 161:503–515

    Article  Google Scholar 

  • Karandashov V, Nagy R, Wegmüller S, Amrhein N, Bucher M (2004) Evolutionary conservation of a phosphate transporter in the arbuscular mycorrhizal symbiosis. Proc Natl Acad Sci USA 101:6285–6290

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kjøller R, Rosendahl S (2001) Molecular diversity of glomalean (arbuscular mycorrhizal) fungi determined as distinct Glomus specific DNA sequences from roots of field grown peas. Mycol Res 105:1027–1032

    Article  Google Scholar 

  • Kubota M, McGonigle TP, Hyakumachi M (2005) Co-occurrence of Arum- and Paris-type morphologies of arbuscular mycorrhizae in cucumber and tomato. Mycorrhiza 15:73–77

    Article  CAS  PubMed  Google Scholar 

  • Landis FC, Gargas A, Givnish TJ (2004) Relationships among arbuscular mycorrhizal fungi, vascular plants and environmental conditions in oak savannas. New Phytol 164:493–504

    Article  Google Scholar 

  • Lovelock CE, Ewel JJ (2005) Links between tree species, symbiotic fungal diversity and ecosystem functioning in simplified tropical ecosystems. New Phytol 167:219–228

    Article  PubMed  Google Scholar 

  • Schüßler A, Geherig H, Schwarzott D, Walker C (2001) Analysis of partial Glomales SSU rRNA gene sequences: implications for primer design and phylogeny. Mycol Res 105:5–15

    Article  Google Scholar 

  • Smith SE, Read DJ (1997) Mycorrhizal symbiosis, 2nd edn. Academic, London

    Google Scholar 

  • Smith FA, Smith SE (1997) Structural diversity in (vesicular)-arbuscular mycorrhizal symbioses. New Phytol 137:373–388

    Article  Google Scholar 

  • Streitwolf-Engel R, Boller T, Wiemken A, Sanders IR (1997) Clonal growth traits of two Prunella species are determined by co-occurring arbuscular mycorrhizal fungi from a calcareous grassland. J Ecol 85:181–191

    Article  Google Scholar 

  • Stukenbrock EH, Rosendahl S (2005) Clonal diversity and population genetic structure of arbuscular mycorrhizal fungi (Glomus spp.) studied by multilocus genotyping of single spores. Mol Ecol 14:743–752

    Article  CAS  PubMed  Google Scholar 

  • Trouvelot A, Kough JL, Gianinazzi-Pearson V (1986) Mesure du taux de mycorhization VA d’un système radiculaire. Recherche de méthodes d’estimation ayant une signification fonctionnelle. In: Gianinazzi-Pearson V, Gianinazzi S (eds) Physiological and genetical aspects of mycorrhizae. INRA, Paris, pp 217–221

    Google Scholar 

  • Trouvelot S, van Tuinen D, Hijiri M, Gianinazzi-Pearson V (1999) Visualization of ribosomal DNA loci in spore interphasic nuclei of Glomalean fungi by fluorescence in situ hybridization. Mycorrhiza 8:203–206

    Article  CAS  Google Scholar 

  • Van Aarle IM, Cavagnaro TR, Smith SE, Smith FA, Dickson S (2005) Metabolic activity of Glomus intraradices in Arum- and Paris-type arbuscular mycorrhizal colonization. New Phytol 166(2):611–618

    Article  PubMed  Google Scholar 

  • Van der Heijden MGA, Boller T, Wiemken A, Sanders IR (1998) Different arbuscular mycorrhizal fungal species are potential determinants of plant community structure. Ecology 79:2082–2091

    Article  Google Scholar 

  • Vandenkoornhuyse P, Husband R, Daniel TJ, Watson IJ, Duck JM, Fitter AH, Young PW (2002) Arbuscular mycorrhizal community composition associated with two plant species in a grassland ecosystem. Mol Ecol 11:1555–1564

    Article  CAS  PubMed  Google Scholar 

  • Vandenkoornhuyse P, Ridgway KP, Watson IJ, Fitter AH, Young JPW (2003) Co-existing grass species have distinctive arbuscular mycorrhizal communities. Mol Ecol 12:3085–3095

    Article  CAS  PubMed  Google Scholar 

  • van Tuinen D, Jacquot E, Zhao B, Gollotte A, Gianinazzi-Pearson V (1998) Characterization of root colonization profiles by a microcosm community of arbuscular mycorrhizal fungi using 25S rDNA-targeted nested PCR. Mol Ecol 7:879–887

    Article  PubMed  Google Scholar 

  • Yamato M, Iwasaki M (2002) Morphological types of arbuscular mycorrhizal fungi in roots of understorey plants in Japanese deciduous broadleaved forests. Mycorrhiza 12:291–296

    Article  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Masanori Nonaka

    Present address: Soil Science Laboratory, Faculty of Agriculture, Niigata University, 8050, Ikarashi-2, Niigata, 950-2181, Japan

Authors and Affiliations

  1. Forestry Research Institute of Ghana, UPO Box 63, UST, Kumasi, Ghana

    Evelyn Matekwor Ahulu

  2. Soil Science Laboratory, Faculty of Agriculture, Niigata University, 8050, Ikarashi-2, Niigata, 950-2181, Japan

    Evelyn Matekwor Ahulu & Hanaka Andoh

Authors
  1. Evelyn Matekwor Ahulu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hanaka Andoh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masanori Nonaka
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masanori Nonaka.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Matekwor Ahulu, E., Andoh, H. & Nonaka, M. Host-related variability in arbuscular mycorrhizal fungal structures in roots of Hedera rhombea, Rubus parvifolius, and Rosa multiflora under controlled conditions. Mycorrhiza 17, 93–101 (2007). https://doi.org/10.1007/s00572-006-0080-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00572-006-0080-7

Keywords

Search

Navigation