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Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation

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Abstract

Recombinants were generated from the ectomycorrhizal basidiomycete, Suillus grevillei, through agroinfection using a binary vector carrying the hygromycin B resistance and the autofluorescent protein, DsRed2, markers. DsRed2 was driven by a cis-regulatory region of the glyceraldeyde-3-phosphate dehydrogenase gene (gpd) from the wood-rotting basidiomycete, Coriolus hirsutus, which contains promoters and 5′ gpd sequences with first through fourth exons and expressed for the first time in Suillus spp. The transformation system and recombinants expressing an autofluorescent protein may be useful in genetic analysis of the symbiosis.

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Acknowledgements

We are grateful to Dr. Mark Guiltinan, the Pennsylvania State University, for provision of Agrobacterium tumefaciens strains. This work was supported by a grant from the Ministry of Agriculture, Forestry and Fishery of Japan.

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Author notes

  1. Takashi Yamazaki

    Present address: Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, Tsukuba, 305-8505, Japan

Authors and Affiliations

  1. Department of Applied Microbiology and Mushroom Science, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan

    Hitoshi Murata & Masahide Sunagawa

  2. Department of Life Science, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, 226-8501, Japan

    Takashi Yamazaki & Kazuo Shishido

  3. Department of Molecular Cell Biology, Forestry & Forest Products Research Institute, Matsunosato 1, Tsukuba, 305-8687, Japan

    Tomohiro Igasaki

Authors
  1. Hitoshi Murata
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  2. Masahide Sunagawa
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  3. Takashi Yamazaki
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  4. Kazuo Shishido
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  5. Tomohiro Igasaki
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Correspondence to Hitoshi Murata.

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Murata, H., Sunagawa, M., Yamazaki, T. et al. Expression of the autofluorescent protein, DsRed2, in the recombinants of the ectomycorrhizal basidiomycete, Suillus grevillei, generated by Agrobacterium-mediated transformation. Mycorrhiza 16, 407–412 (2006). https://doi.org/10.1007/s00572-006-0058-5

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  • DOI: https://doi.org/10.1007/s00572-006-0058-5

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