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Effects of anthracene on development of an arbuscular mycorrhizal fungus and contribution of the symbiotic association to pollutant dissipation

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Abstract

The influence of anthracene, a low molecular weight polycyclic aromatic hydrocarbon (PAH), on chicory root colonization by Glomus intraradices and the effect of the root colonization on PAH degradation were investigated in vitro. The fungus presented a reduced development of extraradical mycelium and a decrease in sporulation, root colonization, and spore germination when exposed to anthracene. Mycorrhization improved the growth of the roots in the medium supplemented containing 140 mg l−1 anthracene, suggesting a positive contribution of G. intraradices to the PAH tolerance of roots. Anthracene disappearance from the culture medium was quantified; results suggested that nonmycorrhizal chicory roots growing in vitro were able to contribute to anthracene dissipation, and in addition, that mycorrhization significantly enhanced anthracene dissipation. These monoxenic experiments demonstrated a positive contribution of the symbiotic association to anthracene dissipation in the absence of other microorganisms. In addition to anthracene dissipation, intracellular accumulation of anthracene was detected in lipid bodies of plant cells and fungal hyphae, indicating intracellular storage capacity of the pollutant by the roots and the mycorrhizal fungus.

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Acknowledgements

Financial support of this work provided by the région Nord/Pas-de-Calais and EU INTERREG project N° 1110/118 is gratefully acknowledged. We wish to thank Amandine Viollet, Ali Siah, and Mohammed Tafraouti for technical assistance and Dr. Gary Robinson for rereading the manuscript.

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  1. Laboratoire de Mycologie/Phytopathologie/Environnement, Université du Littoral Côte d’Opale, BP 699, 62228, Calais cedex, France

    A. Verdin, A. Lounès-Hadj Sahraoui, J. Fontaine, A. Grandmougin-Ferjani & R. Durand

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  1. A. Verdin
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  2. A. Lounès-Hadj Sahraoui
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  3. J. Fontaine
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  4. A. Grandmougin-Ferjani
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Correspondence to A. Grandmougin-Ferjani.

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Verdin, A., Lounès-Hadj Sahraoui, A., Fontaine, J. et al. Effects of anthracene on development of an arbuscular mycorrhizal fungus and contribution of the symbiotic association to pollutant dissipation. Mycorrhiza 16, 397–405 (2006). https://doi.org/10.1007/s00572-006-0055-8

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  • DOI: https://doi.org/10.1007/s00572-006-0055-8

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