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Sulfur uptake in the ectomycorrhizal fungus Laccaria bicolor S238N

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Abstract

The importance of the ectomycorrhiza symbiosis for plant acquisition of phosphorus and nitrogen is well established whereas its contribution to sulfur nutrition is only marginally understood. In a first step to investigate the role of ectomycorrhiza in plant sulfur nutrition, we characterized sulfate and glutathione uptake in Laccaria bicolor. By studying the regulation of sulfate uptake in this ectomycorrhizal fungus, we found that in contrast to bacteria, yeast, and plants, sulfate uptake in L. bicolor was not feedback-inhibited by glutathione. On the other hand, sulfate uptake was increased by sulfur starvation as in other organisms. The activity of 3′-phosphoadenosine 5′-phosphosulfate reductase, the key enzyme of the assimilatory sulfate reduction pathway in fungi, was increased by sulfur starvation and decreased after treatment with glutathione revealing an uncoupling of sulfate uptake and reduction in the presence of reduced sulfur compounds. These results support the hypothesis that L. bicolor increases sulfate supply to the plant by extended sulfate uptake and the plant provides the ectomycorrhizal fungus with reduced sulfur.

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Acknowledgements

We thank Prof. Heinz Rennenberg, Institute of Tree Physiology Freiburg, for his support and critical reading of the manuscript, and the Deutsche Forschungsgemeinschaft for supporting this work in the frame of the priority programme SPP1084 (MolMyk) projects KO2065/2-1 and KO2065/2-2.

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Authors and Affiliations

  1. Albert-Ludwigs-University of Freiburg, Institute of Forest Botany and Tree Physiology, Georges-Köhler-Allee 053, 79110, Freiburg, Germany

    Hounayda Mansouri-Bauly, Jörg Kruse, Zuzana Sýkorová, Ursula Scheerer & Stanislav Kopriva

  2. The School of Forest and Ecosystem Science, University of Melbourne, Melbourne, Australia

    Jörg Kruse

  3. Institute of Botany, University of Basel, Basel, Switzerland

    Zuzana Sýkorová

  4. John Innes Institute, Norwich, UK

    Stanislav Kopriva

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  1. Hounayda Mansouri-Bauly
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  2. Jörg Kruse
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  3. Zuzana Sýkorová
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  4. Ursula Scheerer
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  5. Stanislav Kopriva
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Correspondence to Stanislav Kopriva.

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Mansouri-Bauly, H., Kruse, J., Sýkorová, Z. et al. Sulfur uptake in the ectomycorrhizal fungus Laccaria bicolor S238N. Mycorrhiza 16, 421–427 (2006). https://doi.org/10.1007/s00572-006-0052-y

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  • DOI: https://doi.org/10.1007/s00572-006-0052-y

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