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Root exudates of mycorrhizal tomato plants exhibit a different effect on microconidia germination of Fusarium oxysporum f. sp. lycopersici than root exudates from non-mycorrhizal tomato plants

Mycorrhiza volume 16, pages 365–370 (2006)Cite this article

Abstract

The effect of root exudates from mycorrhizal and non-mycorrhizal tomato plants on microconidia germination of the tomato pathogen Fusarium oxysporum f. sp. lycopersici was tested. Microconidia germination was enhanced in the presence of root exudates from mycorrhizal tomato plants. The more tomato plants were colonized by the arbuscular mycorrhizal fungus Glomus mosseae, the more microconidia germination was increased, indicating that alterations of the exudation pattern depended on the degree of root AM colonization. Moreover, alterations of the exudation pattern of mycorrhizal plants are not only local, but also systemic. Testing the exudates from plants with a high and a low P level revealed that the alterations of the root exudates from mycorrhizal plants, resulting in a changed effect on microconidia germination, are not due to an improved P status of mycorrhizal plants.

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References

  • Akköprü A, Demir S (2005) Biological control of Fusarium wilt in tomato caused by Fusarium oxysporum f. sp. lycopersici by AMF Glomus intraradices and some rhizobacteria. J Phytopathol 153:544–550

    Article  Google Scholar 

  • Caron M, Fortin JA, Richard C (1985) Effect of Glomus intraradices on infection by Fusarium oxysporum f. sp. radicis-lycopersici in tomatoes over a 12-week period. Can J Bot 64:552–556

    Article  Google Scholar 

  • Caron M, Fortin JA, Richard C (1986a) Effect of inoculation sequence on the interaction between Glomus intraradices and Fusarium oxysporum f. sp. radicis-lycopersici in tomatoes. Can J Plant Pathol 8:12–16

    Article  Google Scholar 

  • Caron M, Fortin JA, Richard C (1986b) Effect of phosphorus concentration and Glomus intraradices on Fusarium crown and root rot of tomatoes. Phytopathology 76:942–946

    CAS  Article  Google Scholar 

  • Cordier C, Pozo MJ, Barea JM, Gianinazzi S, Gianinazzi-Pearson V (1998) Cell defense responses associated with localized and systemic resistance to Phytophthora parasitica induced in tomato by an arbuscular mycorrhizal fungus. Mol Plant Microb Interact I 11:1017–1028

    Article  CAS  Google Scholar 

  • Deacon JW (1996) Ecological implications of recognition events in the pre-infection stages of root pathogens. New Phytol 133:135–145

    Article  Google Scholar 

  • Elias KS, Safir GR (1987) Hyphal elongation of Glomus fasciculatus in response to root exudates. Appl Environ Microbiol 53:1928–1933

    PubMed  CAS  Google Scholar 

  • Gericke S, Kurmies B (1952) Die kolorimetrische Phosphorsäure-bestimmung mit Ammonium – Vanadat – Molybdat und ihre Anwendung in der Pflanzenanalyse. Z Pflanzenernähr, Düngung, Bodenkunde 59:235–247

    CAS  Google Scholar 

  • Jones DL, Hodge A, Kuzyakov Y (2004) Plant and mycorrhizal regulation of rhizodeposition. New Phytol 163:459–480

    Article  CAS  Google Scholar 

  • Lagopodi AL, Ram AFJ, Lamers GEM, Punt PJ, Van den Hondel CAMJ, Lugtenberg BJJ, Bloemberg GV (2002) Novel aspects of tomato root colonization and infection by Fusarium oxysporum f. sp. radicis-lycopersici revealed by confocal laser scanning microscopic analysis using the green flourescent protein as a marker. Mol Plant Microb Interact I 15:172–179

    Article  CAS  Google Scholar 

  • Lioussanne L, Jolicoeur M, St. Arnaud M (2003) Effects of the alteration of tomato root exudation by Glomus intraradices colonization on Phytophthora parasitica var. Nicotianae zoospores. Abstract No. 253, Abstract Book ICOM 4; Montreal, Canada, p 291

  • Marschner P, Baumann K (2003) Changes in bacterial community structure induced by mycorrhizal colonisation in split-root maize. Plant Soil 251:279–289

    Article  CAS  Google Scholar 

  • Newman EI (1966) A method of estimating the total length of root in a sample. J Appl Ecol 3:139–145

    Article  Google Scholar 

  • Norman JR, Hooker JE (2000) Sporulation of Phytophthora fragariae shows greater stimulation by exudates of non-mycorrhizal than by mycorrhizal strawberry roots. Mycol Res 104:1069–1073

    Article  Google Scholar 

  • Pinior A, Wyss U, Piché Y, Vierheilig H (1999) Plants colonized by AM fungi regulate further root colonization by AM fungi through altered root exudation. Can J Bot 77:891–897

    Article  Google Scholar 

  • Rodríguez-Gálvez E, Mendgen K (1995) The infection process of Fusarium oxysporum in cotton root tips. Protoplasma 189:61–72

    Article  Google Scholar 

  • Ryan A, Jones P (2004) The effect of mycorrhization of potato roots on the hatching chemicals active towards the potato cyst nematodes, Globodera pallida and G. rostochiensis. Nematology 6:335–342

    Article  Google Scholar 

  • Smith SE, Read DJ (1997) Mycorrhizal symbiosis. Academic, London

    Google Scholar 

  • Sood SG (2003) Chemotactic response of plant-growth-promoting bacteria towards roots of vesicular–arbuscular mycorrhizal tomato plants. FEMS Microbiol Ecol 45:219–227

    Article  CAS  Google Scholar 

  • Steinkellner S, Mammerler R, Vierheilig H (2005) Microconidia germination of the tomato pathogen Fusarium oxysporum in the presence of root exudates. J Plant Interact 1:23–30

    Article  CAS  Google Scholar 

  • Tawaraya K, Watanabe S, Yoshida E, Wagatsuma T (1996) Effect of onion (Allium cepa) root exudates on the hyphal growth of Gigaspora margarita. Mycorrhiza 6:57–59

    Article  Google Scholar 

  • Tawaraya K, Hashimoto K, Wagatsuma T (1998) Effect of root exudate fractions from P-deficient and P-sufficient onion plants on root colonisation by the arbuscular mycorrhizal fungus Gigaspora margarita. Mycorrhiza 8:67–70

    Article  CAS  Google Scholar 

  • Vierheilig H (2004a) Regulatory mechanisms during the plant-arbuscular mycorrhizal fungus interaction. Can J Bot 82:1166–1176

    Article  CAS  Google Scholar 

  • Vierheilig H (2004b) Further root colonization by arbuscular mycorrhizal fungi in already mycorrhizal plants is suppressed after a critical level of root colonization. J Plant Physiol 161:339–341

    Article  PubMed  CAS  Google Scholar 

  • Vierheilig H, Piché Y (2002) Signalling in arbuscular mycorrhiza: facts and hypotheses. In: Buslig B, Manthey J (eds) Flavonoids in cell function. Kluwer Academic/Plenum, New York, pp 23–39

    Google Scholar 

  • Vierheilig H, Coughlan AP, Wyss U, Piché Y (1998) Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Appl Environ Microbiol 64:5004–5007

    PubMed  CAS  Google Scholar 

  • Vierheilig H, Lerat S, Piché Y (2003) Systemic inhibition of arbuscular mycorrhiza development by root exudates of cucumber plants colonized by Glomus mosseae. Mycorrhiza 13:167–170

    Article  PubMed  CAS  Google Scholar 

  • Wyss P, Boller T, Wiemken A (1991) Phytoalexin response is elicited by a pathogen (Rhizoctonia solani) but not by a mycorrhizal fungus (Glomus mosseae) in bean roots. Experientia 47:395–399

    Article  CAS  Google Scholar 

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Acknowledgement

We thank Ms. E. Holub, Institute of Plant Production, BOKU, for P analysis.

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  1. Institut für Pflanzenschutz, Department für Angewandte Pflanzenwissenschaften, und Pflanzenbiotechnologie, Universität für Bodenkultur Wien, Peter-Jordan-Str. 82, A-1190, Wien, Austria

    S. Scheffknecht, R. Mammerler, S. Steinkellner & H. Vierheilig

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  1. S. Scheffknecht
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  2. R. Mammerler
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  3. S. Steinkellner
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  4. H. Vierheilig
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Correspondence to S. Steinkellner.

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Scheffknecht, S., Mammerler, R., Steinkellner, S. et al. Root exudates of mycorrhizal tomato plants exhibit a different effect on microconidia germination of Fusarium oxysporum f. sp. lycopersici than root exudates from non-mycorrhizal tomato plants. Mycorrhiza 16, 365–370 (2006). https://doi.org/10.1007/s00572-006-0048-7

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  • DOI: https://doi.org/10.1007/s00572-006-0048-7

Keywords

  • Arbuscular mycorrhiza
  • Glomales
  • Fusarium oxysporum
  • Microconidia
  • Tomato
  • Root exudates