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Accumulation of reactive oxygen species in arbuscular mycorrhizal roots

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Abstract

We investigated the accumulation of reactive oxygen species (ROS) in arbuscular mycorrhizal (AM) roots from Medicago truncatula, Zea mays and Nicotiana tabacum using three independent staining techniques. Colonized root cortical cells and the symbiotic fungal partner were observed to be involved in the production of ROS. Extraradical hyphae and spores from Glomus intraradices accumulated small levels of ROS within their cell wall and produced ROS within the cytoplasm in response to stress. Within AM roots, we observed a certain correlation of arbuscular senescence and H2O2 accumulation after staining by diaminobenzidine (DAB) and a more general accumulation of ROS close to fungal structures when using dihydrorhodamine 123 (DHR 123) for staining. According to electron microscopical analysis of AM roots from Z. mays after staining by CeCl3, intracellular accumulation of H2O2 was observed in the plant cytoplasm close to intact and collapsing fungal structures, whereas intercellular H2O2 was located on the surface of fungal hyphae. These characteristics of ROS accumulation in AM roots suggest similarities to ROS accumulation during the senescence of legume root nodules.

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Acknowledgements

The authors thank Gerlinde Waiblinger and Regina Franke for their excellent technical assistance and the “Deutsche Forschungsgemeinschaft” for financial support.

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Authors and Affiliations

  1. Leibniz-Institut für Pflanzenbiochemie, Weinberg 3, 06120, Halle (Saale), Germany

    T. Fester

  2. Biozentrum der Universität Halle, Weinbergweg 22, 06120, Halle (Saale), Germany

    G. Hause

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  1. T. Fester
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  2. G. Hause
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Correspondence to T. Fester.

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Fester, T., Hause, G. Accumulation of reactive oxygen species in arbuscular mycorrhizal roots. Mycorrhiza 15, 373–379 (2005). https://doi.org/10.1007/s00572-005-0363-4

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