Abstract
We report for the first time the arbuscular mycorrhizal (AM) status of native plant species and AM fungal diversity in the grasslands of southern Tibet. A total of 51 soil samples were collected from the rhizospheres of the dominant plant species, and AM fungal structures were observed in 18 (82%) of 22 plant species examined. Vesicles and aseptate hyphae were the structures most frequently observed in the plant roots. After trap culture for 5 months, 25 AM fungal taxa were identified in the soil samples collected, of which nine belonged to Glomus, ten to Acaulospora, one to Entrophospora and five to Scutellospora. The frequency of occurrence of different genera and species varied greatly. Glomus was the dominant genus, and the most frequent and abundant species was Glomus mosseae. Over the whole sampling area, spore density in the rhizosphere soil of different host plant species ranged from 2 to 66 per 20 g air-dried soil. Overall AM fungal species richness was 2.10 and species diversity was 2.35. AM fungal diversity was also compared among the four different land use types (farmland and normal, disturbed and highly disturbed montane scrub grassland). Spore densities in the farmland and normal grassland were much higher than in the grasslands that had been degraded to varying degrees. The species richness in normal grassland was the highest of the four land use types examined. Species diversity varied from 1.99 to 0.94 and was highest in normal grassland, intermediate in degraded grassland and farmland, and lowest in the highly disturbed grassland.
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Anken T, Weisskopf P, Zihlmann U, Forrer H, Jansa J, Perhacova K (2004) Long-term tillage system effects under moist cool conditions in Switzerland. Soil Tillage Res 78:171–183
Azcón-Aguilar C, Palenzuela EJ, Roldan A, Bautista S, Vallejo R, Barea JM (2002) Analysis of the mycorrhizal potential in the rhizosphere of representative plant species from desertification-threatened Mediterranean shrublands. Appl Soil Ecol 21:1–9
Bever JD (2002) Host-specificity of AM fungal population growth rates can generate feedback on plant growth. Plant Soil 244:281–290
Bever D, Morton JB, Antonovics J, Schultz PA (1996) Host-dependent sporulation and species diversity of arbuscular mycorrhizal fungi in a mown grassland. J Ecol 84:71–82
Bever JD, Schultz PA, Pringle A, Morton JB (2001) Arbuscular mycorrhizal fungi: more diverse than meets the eye, and the ecological tale of why. Bioscience 51:923–931
Boddington CL, Dodd JC (2000) The effect of agricultural practices on the development of indigenous arbuscular mycorrhizal fungi. I. Field studies in an Indonesian ultisol. Plant Soil 218:137–144
Burrows RL, Pfleger FL (2002) Arbuscular mycorrhizal fungi respond to increasing plant diversity. Can J Bot 80:120–130
Daniels BA, Skipper HD (1982) Methods for the recovery and quantitative of propagules from soil. In: NC Schenck (ed) Methods and principles of mycorrhizal research. American Phytopathological Society, St. Paul, MN, pp 29–35
Ferrol N, Calvente R, Cano C, Barea JM, Azcón-Aguilar C (2004) Analysing arbuscular mycorrhizal fungal diversity in shrub-associated resource islands from a desertification threatened semiarid Mediterranean ecosystem. Appl Soil Ecol 25:123–133
Graham JH (2001) What do root pathogens see in mycorrhizas? New Phytol 148:357–359
Hartnett DC, Wilson GWT (2002) The role of mycorrhizas in plant community structure and dynamics: lessons from grasslands. Plant Soil 244:319–331
Hirsch AM, Kapulnik Y (1998) Signal transduction pathways in mycorrhizal associations: comparison with the Rhizobium-legume symbiosis. Fungal Genet Biol 23:205–212
Jansa J, Mozafar A, Anken T, Ruh R, Sanders IR, Frossard E (2002) Diversity and structure of AMF communities as affected by tillage in a temperate soil. Mycorrhiza 12:225–234
Jasper DA, Abbot LK, Robson AD (1991) The effect of soil disturbance on vesicular-arbuscular mycorrhizal fungi in soils from different vegetation types. New Phytol 118:471–476
Jayachandran K, Shetty KG (2003) Growth response and phosphorus uptake by arbuscular mycorrhizae of wet prairie sawgrass. Aquat Bot 76:281–290
Johnson NC (1993) Can fertilization of soil select less mutualistic mycorrhizae? Ecol Appl 3:749–757
Klironomos JN, Hart MM, Gurney JE, Moutoglis P (2001) Interspecific differences in the tolerance of arbuscular mycorrhizal fungi to freezing and drying. Can J Bot 79:1161–1166
Koide R, Dickie IA (2002) Effects of mycorrhizal fungi on plant populations. Plant Soil 244:307–317
Mullen RB, Schmidt SK (1993) Mycorrhizal infection, phosphorus uptake and phenology in Ranunculus adoneus: implications for the functioning of mycorrhizas in alpine systems. Oecologia 94:229–234
Muthukumar T, Udaiyan K, Shanmughavel P (2004) Mycorrhiza in sedges: an overview. Mycorrhiza 14:65–67
O’Connor PJ, Smith SE, Smith FA (2002) Arbuscular mycorrhizas influence plant diversity and community structure in a semiarid herbland. New Phytol 154:209–218
Phillips JM, Hayman DS (1970) Improved procedures for cleaning and staining parasitic and vesicular arbuscular mycorrhizal fungi for rapid assessment of infection. Trans Br Mycol Soc 55:158–160
Read DJ, Haselwandter K (1981) Observation on the mycorrhizal status of some alpine plant communities. New Phytol 88:341–353
Richter BS, Tiller RL, Stutz JC (2002) Assessment of arbuscular mycorrhizal fungal propagules and colonization from abandoned agricultural fields and semi–arid grasslands in riparian floodplains. Appl Soil Ecol 20:227–238
Schenck NC, Perez Y (1990) Manual for the identification of vesicular-arbuscular mycorrhizal fungi. INVAM, University of Florida, Gainesville, FL, USA
Smith MR, Charvat I, Jacobson RL (1998) Arbuscular mycorrhizae promote establishment of prairie species in a tallgras prairie restoration. Can J Bot 76:1947–1954
Stutz JC, Morton JB (1996) Successive pot cultures reveal high species richness of arbuscular endomycorrhizal fungi in arid ecosystems. Can J Bot 74:1883–1889
Stutz JC, Copeman R, Martin CA, Morton JB (2000) Patterns of species composition and distribution of arbuscular mycorrhizal fungi in arid regions of southwestern North America and Namibia, Africa. Can J Bot 78:237–245
van der Heijden MGA, Klironomos JN, Ursic M, Moutoglis P, Streitwolf-Engel R, Boller T, Wiemken A, Sanders IR (1998) Mycorrhizal fungal diversity determines plant biodiversity, ecosystem variability and productivity. Nature 396:69–72
Zhang MQ, Wang YS, Zhang C (1994) The ecological distribution of AM fungi in North China. Mycosystema 13:166–172
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We thank the Nature Science Foundation of China (40571078 and 30470341) and the Royal Society (China Exchanges Project 15360) for financial support.
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Gai, J.P., Feng, G., Cai, X.B. et al. A preliminary survey of the arbuscular mycorrhizal status of grassland plants in southern Tibet. Mycorrhiza 16, 191–196 (2006). https://doi.org/10.1007/s00572-005-0032-7
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DOI: https://doi.org/10.1007/s00572-005-0032-7