Microsystem Technologies

, Volume 24, Issue 6, pp 2847–2852 | Cite as

Rapid prototyping of flexible multilayer microfluidic devices using polyester sealing film

  • Yiqiang Fan
  • Shicheng Liu
  • Jianyun He
  • Kexin Gao
  • Yajun Zhang
Technical Paper


In this study, we proposed a novel fabrication method for rapid prototyping of multilayer flexible microfluidic devices using laser ablated polyester sealing films. The polyester sealing film (adhesive seals) is originally used for the sealing of PCR well plates during the amplification of DNA, the PCR sealing film is found highly suitable as the bulk material for microfluidic devices in this study, due to the excellent biocompatibility, wide range of working temperatures, low-cost, self-adhesive and optical transmittance in the visible range. In this proposed fabrication method, the CO2 laser is used for the ablation of microchannels and alignment marks on the PCR sealing film, and a simple custom-made aliment tool was used for the alignment and bonding. The secured bonding between layers was achieved easily with the PCR sealing film’s own adhesives. For the demonstration of the proposed technique, various microfluidic devices were designed and fabricated, bonding strength test was also provided in this study.



This work was supported by the National Science Foundation of China (51573017), and Fundamental Research Funds for the Central Universities (buctrc201609).


  1. Basova EY, Foret F (2015) Droplet microfluidics in (bio)chemical analysis. Analyst 140:22–38. CrossRefGoogle Scholar
  2. Boos JA, Beuvink I (2016) Whole-body scanning PCR, a tool for the visualization of the in vivo biodistribution pattern of endogenous and exogenous oligonucleotides in rodents. Springer, New YorkCrossRefGoogle Scholar
  3. Cai J, Jiang J, Gao F, Jia G, Zhuang J, Tang G, Fan Y (2017) Rapid prototyping of cyclic olefin copolymer based microfluidic system with CO2 laser ablation. Microsyst Technol 23(10):5063–5069CrossRefGoogle Scholar
  4. Chen YL, Jiang HR (2017) Particle concentrating and sorting under a rotating electric field by direct optical-liquid heating in a microfluidics chip. Biomicrofluidics 11:034102CrossRefGoogle Scholar
  5. Christiansen L, Amini S, Zhang F, Ronaghi M, Gunderson KL, Steemers FJ (2017) Contiguity-preserving transposition sequencing (CPT-seq) for genome-wide haplotyping, assembly, and single-cell ATAC-seq. Methods Mol Biol 1551:207CrossRefGoogle Scholar
  6. Didar TF, Li K, Tabrizian M, Veres T (2013) High throughput multilayer microfluidic particle separation platform using embedded thermoplastic-based micropumping. Lab Chip 13:2615CrossRefGoogle Scholar
  7. Duncombe TA, Tentori AM, Herr AE (2015) Microfluidics: reframing biological enquiry. Nat Rev Mol Cell Biol 16:554CrossRefGoogle Scholar
  8. Fan Y, Li H, Yi Y, Foulds IG (2014) PMMA to polystyrene bonding for polymer based microfluidic systems. Microsyst Technol 20:59–64CrossRefGoogle Scholar
  9. Han J et al (2017) A fully integrated microchip system for automated forensic short tandem repeat analysis. Analyst 142:2004–2012CrossRefGoogle Scholar
  10. He W, Xiao J, Zhang Z, Zhang W, Cao Y, He R, Chen Y (2015) One-step electroplating 3D template with gradient height to enhance micromixing in microfluidic chips. Microfluid Nanofluid 19:1–8CrossRefGoogle Scholar
  11. Isiksacan Z, Tahsin Guler M, Aydogdu B, Bilican I, Elbuken C (2016) Rapid fabrication of microfluidic PDMS devices from reusable PDMS molds using laser ablation. J Micromech Microeng 26:035008CrossRefGoogle Scholar
  12. Jathoul AP et al (2015) Deep in vivo photoacoustic imaging of mammalian tissues using a tyrosinase-based genetic reporter. Nat Photonics 9:239CrossRefGoogle Scholar
  13. Kim D, Yoo JH, Lee J-B (2016) Liquid metal-based reconfigurable and stretchable photolithography. J Micromech Microeng 26:045004. CrossRefGoogle Scholar
  14. Li J et al (2012) Fabrication of a thermoplastic multilayer microfluidic chip. J Mater Process Technol 212:2315–2320. CrossRefGoogle Scholar
  15. Liga A, Morton JAS, Kersaudy-Kerhoas M (2016) Safe and cost-effective rapid-prototyping of multilayer PMMA microfluidic devices. Microfluid Nanofluid. Google Scholar
  16. Mauk MG, Liu C, Qiu X, Chen D, Song J, Bau HH (2017) Microfluidic “pouch” chips for immunoassays and nucleic acid amplification tests. Methods Mol Biol 1572:467CrossRefGoogle Scholar
  17. Patko D, Mártonfalvi Z, Kovacs B, Vonderviszt F, Kellermayer M, Horvath R (2014) Microfluidic channels laser-cut in thin double-sided tapes: cost-effective biocompatible fluidics in minutes from design to final integration with optical biochips. Sens Actuators B Chem 196:352–356CrossRefGoogle Scholar
  18. Pham P, Vo T, Luo X (2016) Steering air bubbles with an add-on vacuum layer for biopolymer membrane biofabrication in PDMS microfluidics. Lab Chip 17:248CrossRefGoogle Scholar
  19. Roy S, Yue CY, Lam YC, Wang ZY, Hu H (2010) Surface analysis, hydrophilic enhancement, ageing behavior and flow in plasma modified cyclic olefin copolymer (COC)-based microfluidic devices. Sens Actuators B Chem 150:537–549CrossRefGoogle Scholar
  20. Schaff UY, Sommer GJ (2011) Whole blood immunoassay based on centrifugal bead sedimentation. Clin Chem 57:753–761CrossRefGoogle Scholar
  21. Serra M, Pereiro I, Yamada A, Viovy JL, Descroix S, Ferraro D (2017) A simple and low-cost chip bonding solution for high pressure, high temperature and biological applications. Lab Chip 17:629–634CrossRefGoogle Scholar
  22. Wu HC, Lyau JB, Lin MH, Chuang YJ, Chen H (2017) Multilayer microfluidic systems with indium-tin-oxide microelectrodes for studying biological cells. J Micromech Microeng. CrossRefGoogle Scholar
  23. Yu H, Chong ZZ, Tor SB, Liu E, Loh NH (2015) Low temperature and deformation-free bonding of PMMA microfluidic devices with stable hydrophilicity via oxygen plasma treatment and PVA coating. RSC Adv 5:8377–8388CrossRefGoogle Scholar
  24. Zhang Z, Luo Y, Wang X, Zheng Y, Zhang Y, Wang L (2010) Thermal assisted ultrasonic bonding of multilayer polymer microfluidic devices. J Micromech Microeng 20:015036. CrossRefGoogle Scholar
  25. Zhang H, Liu X, Li T, Han X (2014) Miscible organic solvents soak bonding method use in a PMMA multilayer microfluidic device. Micromachines 5:1416–1428. CrossRefGoogle Scholar
  26. Zia AB, Ali MA, Zeb MO, Shafiq U, Fida SR, Ahmed N (2017) Development of microfluidic lab-on-disc based portable blood testing point-of-care diagnostic device. In: Biomedical engineering and sciences, pp 142–145Google Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Yiqiang Fan
    • 1
  • Shicheng Liu
    • 1
  • Jianyun He
    • 1
  • Kexin Gao
    • 1
  • Yajun Zhang
    • 1
  1. 1.School of Mechanical and Electrical EngineeringBeijing University of Chemical TechnologyBeijingChina

Personalised recommendations