Fabrication and hemocompatibility of carboxy-chitosan stabilized magnetite nanoparticles
- 146 Downloads
This paper describes fabrication of a new hemocompatible, magnetic nanoparticles based on magnetite and naturally occurring chitosan, potentially applicable as biomaterials to nanobiomedicine. We fabricated carboxy-functionalized magnetite–chitosan (Fe3O4–CS–BTCDA) nanocomposite particles by a simple two-stage protocol. Magnetite–chitosan (Fe3O4–CS) nanocomposite particles were first prepared via in situ chemical coprecipitation reactions using Fe2+ and Fe3+ salts in an alkaline aqueous solution of CS. Fe3O4–CS nanocomposite particles were then reacted with butanetetracarboxylic dianhydride (BTCDA) to obtain the Fe3O4–CS–BTCDA nanocomposite particles dispersible under physiological conditions. The obtained nanoparticles are superparamagnetic. The hemolytic activity of the Fe3O4–CS–BTCDA nanocomposite particles is very low and essential for practical bio-related applications.
The authors thank Mr. Nobuaki Kutsuzawa and Dr. Osamu Ishii of Yamagata University for instruction on the magnetic measurements, and Dr. Shigekazu Yano of Yamagata University for his kind assistance on the hemocompatibility analysis. Md. Abdur Rahman also acknowledges the financial support from the Ministry of Education, Culture, Sports, Science, and Technology, Japan.
- Abreu F, Campana-Filho SP (2005) Preparation and characterization of carboxymethylchitosan. Polímeros: Ciência e Tecnologia 15:79–83Google Scholar
- Arias JL, Reddy LH, Couvreur P (2012) Fe3O4/chitosan nanocomposite for magnetic drug targeting to cancer. J Appl Phys 109:084303–084311Google Scholar
- Cornell RM, Schwertmann U (1996) The iron oxides: structure, properties, reactions, occurrence and uses. Wiley-VCH, WeinheimGoogle Scholar
- Hritcu D, Popa M, Popa N, Badescu V, Balan V (2009) Preparation and characterization of magnetic chitosan nanospheres. Turk J Chem 33:785–796Google Scholar
- Kariminia S, Shamsipuri A, Shamsipuri M (2016) Analytical characteristics and application of novel chitosan coated magnetic nanoparticles as an efficient drug delivery system for ciprofloxacin. Enhanced drug release kinetics by low-frequency ultrasounds. J Pharm Biomed Anal 129:450–457CrossRefGoogle Scholar
- Liu X, Chen X, Li Y, Wang X, Peng X, Zhu W (2012) Preparation of superparamagnetic Fe3O4@alginate/chitosan nanospheres for candida rugosa lipase immobilization and utilization of layer-by-layer assembly to enhance the stability of immobilized lipase. ACS Appl Mater Inter 4:5169–5178CrossRefGoogle Scholar
- López GR, Pineda GM, Hurtado G, de León DR, Fernández S, Saade H, Bueno D (2013) Chitosan-coated magnetic nanoparticles prepared in one step by reverse microemulsion precipitation. Int J Mol Sci 141:9636–19650Google Scholar
- Sadighian S, Hosseini-Monfared S, Rostamizadeh K, Hamidi M (2015) pH-Triggered magnetic-chitosan nanogels (MCNs) for doxorubicin delivery: physically vs. chemically cross linking approach. Adv Pharm Bull 5:115–120Google Scholar
- Salazar-Alvarez G (2004) Synthesis, characterization and application of iron oxide nanoparticles. Doctoral Thesis, Stockholm, SwedenGoogle Scholar
- Samadikhah HR, Majidi A, Nikkhah M, Hosseinkhani S (2011) Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes. Int J Nanomed 6:2275–2283Google Scholar
- Shrifian-Esfahni A, Salehi TM, Nasr-Esfahni M, Ekramian E (2015) Chitosan-modified superparamagnetic iron oxide nanoparticles: design, fabrication, characterization and antibacterial activity. CHEMIK 69:19–32Google Scholar
- Thakur M, De K, Giri S, Si S, Kotal A, Mandal KT (2006) Interparticle interaction and size effect in polymer coated magnetite nanoparticles. J Phys: Condens Matter 98:9093–9104Google Scholar