Microwave atomic force microscope: MG63 osteoblast-like cells analysis on nanometer scale
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In this paper, we report a non-invasive and non-destructive probing method for analyzing the MG63 osteoblast-like cells. High frequency microwave atomic force microscope (M-AFM) can be used to measure the surface topography and microwave image of MG63 cells simultaneously in one scanning process. Under the frequency modulation AFM mode, the M-AFM probe tip can scan above the cell surface, maintaining a constant stand-off distance and the created lateral forces were small enough as not to sweep away or deform the fragile biomolecules. By analyzing the results, quantification such as, the number and distribution of organelles and proteins of MG63 cells as well as their dimension and electrical property information can be characterized. The unique potentials of that M-AFM imaging biological substrates with no damaging manner and nanometer scale resolution, while the original structure and function of the biomolecules during the investigation are preserved, make this technique very attractive to biologists.
KeywordsAtomic Force Microscopy MG63 Cell Microwave Signal Residual Body Intracellular Structure
This work was supported by the Japan Society for the Promotion of Science under Grants-in-Aid for Scientific Research (A) 26249001.
- Briegel A, Ortega DR, Tocheva EI, Wuichet K, Li Z, Chen S, Müller A, Iancu CV, Murphy GE, Dobroa MJ, Zhulin IB, Jensen GJ (2009) Universal architecture of bacterial chemoreceptor arrays. Proc Natl Acad Sci 05:181106Google Scholar
- Cremer C, Cremer T (1978) Considerations on a laser-scanning-microscope with high resolution and depth of field. Microsc Acta 81:31Google Scholar
- Ju Y, Sato H, Soyama H (2005) Fabrication of the tip of GaAs microwave probe by wet etching. In: Proceedings of IPACK 2005, p 73140Google Scholar
- Matsumoto B (2002) Cell biological applications of confocal microscopy, Academic Press, New YorkGoogle Scholar
- Segura-Valdez ML, Zamora-Cura A, Gutiérrez-Quintanar N, Villalobos-Nájera E, Rodríguez-Vázquez JB, Galván-Arrieta TC, Jiménez-Rodríguez D, Agredano-Moreno LT, Lara-Martínez R, Jiménez-García LF (2010) Visualization of cell structure in situ by atomic force microscopy. In: Méndez-Vilas A, Díaz J (eds) Microscopy: science, technology, applications and education, vol 1. Formatex Research Center, Badajoz, pp 441–448Google Scholar
- Tsilimbaris MK, Lesniewska E, Lydataki S, Le Grimellec C, Goudonnet JP, Pallikaris IG (2000) The use of atomic force microscopy for the observation of corneal epithelium surface. Invest Ophthalmol Vis Sci 41:680Google Scholar
- Tumminia SJ, Mitton KP, Arora J, Zelenka P, Epstein DL, Russell P (1998) Mechanical stretch alters the actin cytoskeletal network and signal transduction in human trabecular meshwork cells. Invest Ophthalmol Vis Sci 39:1361Google Scholar