Microsystem Technologies

, Volume 22, Issue 3, pp 603–608 | Cite as

Microwave atomic force microscope: MG63 osteoblast-like cells analysis on nanometer scale

  • Lan Zhang
  • Yuanhui Song
  • Atsushi Hosoi
  • Yasuyuki Morita
  • Yang Ju
Technical Paper


In this paper, we report a non-invasive and non-destructive probing method for analyzing the MG63 osteoblast-like cells. High frequency microwave atomic force microscope (M-AFM) can be used to measure the surface topography and microwave image of MG63 cells simultaneously in one scanning process. Under the frequency modulation AFM mode, the M-AFM probe tip can scan above the cell surface, maintaining a constant stand-off distance and the created lateral forces were small enough as not to sweep away or deform the fragile biomolecules. By analyzing the results, quantification such as, the number and distribution of organelles and proteins of MG63 cells as well as their dimension and electrical property information can be characterized. The unique potentials of that M-AFM imaging biological substrates with no damaging manner and nanometer scale resolution, while the original structure and function of the biomolecules during the investigation are preserved, make this technique very attractive to biologists.


Atomic Force Microscopy MG63 Cell Microwave Signal Residual Body Intracellular Structure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the Japan Society for the Promotion of Science under Grants-in-Aid for Scientific Research (A) 26249001.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Lan Zhang
    • 1
  • Yuanhui Song
    • 1
  • Atsushi Hosoi
    • 1
  • Yasuyuki Morita
    • 1
  • Yang Ju
    • 1
  1. 1.Department of Mechanical Science and EngineeringNagoya UniversityNagoyaJapan

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