Abstract
This paper presents a new air-bubble free microfluidic blood cuvette for the measurement of hemoglobin concentration. The microfluidic blood cuvette was filled with blood samples by capillary force, and hemoglobin levels in the blood were determined by measuring absorbance at the wavelength of 530 nm. Two different microfluidic blood cuvettes with dual and single sidewall microchannels were investigated. The microfluidic blood cuvette was fabricated using a polymethyl methacrylate substrate and a dry film photoresist. During the blood-filling process, air was trapped in the dual-sided wall-type cuvettes, while no air trapping occurred in the single sidewall-type cuvettes. The sensitivity of the hemoglobin measurements was more linear in a 105 μm deep microchannel than in a 35 μm deep microchannel.
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Acknowledgments
This study was supported by the Ministry of Education, Science and Technology and the Korea Institute for Advancement of Technology through the Human Resource Training Project for Regional Innovation and supported by the Human Resources Development program (No. 20134030200240) of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) grant funded by the Korea government Ministry of Trade, Industry and Energy. This work was also supported by Kangwon National University, South Korea.
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Kim, H.J., Son, J.K., Seo, Y.H. et al. Disposable microfluidic blood cuvette for measuring hemoglobin concentration. Microsyst Technol 20, 499–504 (2014). https://doi.org/10.1007/s00542-013-1954-1
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DOI: https://doi.org/10.1007/s00542-013-1954-1