Abstract
Microfluidic chips were designed and fabricated to capture cells in a relative small volume to generate the desired concentration needed for analysis. The microfluidic chips comprise three-dimensional (3-D) cell capture structures array fabricated in PDMS. The capture structure includes two layers. The first layer consists of spacers to create small gap between the upper layer and glass. The second layer is a sharp corner U-shaped compartment with sharp corners at the fore-end. And another type capture structure with Y-shaped fluidic guide has been designed. It was demonstrated that the structures can capture cells in theory, using Darcy–Weisbach equation and COMSOL Multiphysics. Then yeast cell was chosen to test the performance of the chips. The chip without fluid guides captured ~1.44 × 105 cells and the capture efficiency was up to 71 %. And the chip with fluid guides captured ~5.0 × 104 cells and the capture efficiency was ~25 %. The chip without fluid guides can capture more cells because the yeast cells in the chip without fluid guides are subject to larger hydrodynamic drag force.
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Abbreviations
- f :
-
Darcy friction factor
- l :
-
Length of channel
- v :
-
Fluidic velocity
- d :
-
Hydraulic diameter
- ρ :
-
Fluid density
- S :
-
Cross-sectional area of channel
- Q :
-
Volumetric flow rate
- β :
-
Aspect ratio
- R e :
-
Reynolds number
- η :
-
Fluid viscosity
- w :
-
Width of channel
- h :
-
Height of channel
- r :
-
Radius of cell
- F drag :
-
Hydrodynamic drag force
References
Bao N, Lu C (2008) A microfluidic device for physical trapping and electrical lysis of bacterial cells. Appl Phys Lett 92:214103
Celata G, Cumo M, McPhail S, Zummo G (2006) Characterization of fluid dynamic behaviour and channel wall effects in microtube. Int J Heat Fluid Flow 27:135–143
Chen JD, Chen D, Xie Y, Yuan T, Chen X (2013) Progress of microfluidics for biology and medicine. Nano-Micro Lett 5:66–80
Chung J, Kim YJ, Yoon E (2011) Highly-efficient single-cell capture in microfluidic array chips using differential hydrodynamic guiding structures. Appl Phys Lett 98:123701
Demello AJ (2006) Control and detection of chemical reactions in microfluidic systems. Nature 442:394–402
Di Carlo D, Wu LY, Lee LP (2006) Dynamic single cell culture array. Lab Chip 6:1445–1449
Faley S, Seale K, Hughey J, Schaffer DK, VanCompernolle S, McKinney B, Baudenbacher F, Unutmaz D, Wikswo JP (2008) Microfluidic platform for real-time signaling analysis of multiple single T cells in parallel. Lab Chip 8:1700–1712
Jäggi RD, Sandoz R, Effenhauser CS (2007) Microfluidic depletion of red blood cells from whole blood in high-aspect-ratio microchannels. Microfluid Nanofluid 3:47–53
Laurell T, Petersson F, Nilsson A (2006) Chip integrated strategies for acoustic separation and manipulation of cells and particles. Chem Soc Rev 36:492–506
Lim YC, Kouzani AZ, Duan W (2010) Lab-on-a-chip: a component view. Microsyst Technol 16:1995–2015
Lin WY, Lin YH, Lee GB (2010) Separation of micro-particles utilizing spatial difference of optically induced dielectrophoretic forces. Microfluid Nanofluid 8:217–229
Lombardi D, Dittrich PS (2010) Advances in microfluidics for drug discovery. Expert Opin Drug Discovery 5:1081–1094
Murata M, Okamoto Y, Park YS, Kaji N, Tokeshi M, Baba Y (2009) Cell separation by the combination of microfluidics and optical trapping force on a microchip. Anal Bioanal Chem 394:277–283
Sakar MS, Steager EB, Kim MJ, Pappas GJ, Kumar V (2010) Single cell manipulation using ferromagnetic composite microtransporters. Appl Phys Lett 96:043705
Sia SK, Whitesides GM (2003) Microfluidic devices fabricated in poly (dimethylsiloxane) for biological studies. Electrophoresis 24:3563–3576
Skelley AM, Kirak O, Suh H, Jaenisch R, Voldman J (2009) Microfluidic control of cell pairing and fusion. Nat Methods 6:147–152
Song Y, Hormes J, Kumar CSSR (2008) Microfluidic synthesis of nanomaterials. Small 4:698–711
Tan WH, Takeuchi S (2007) A trap-and-release integrated microfluidic system for dynamic microarray applications. Natl Acad Sci USA 104:1146–1151
Wlodkowic D, Faley S, Zagnoni M, Wikswo JP, Cooper JM (2009) Microfluidic single-cell array cytometry for the analysis of tumor apoptosis. Anal Chem 81:5517–5523
Wu HW, Lin CC, Lee GB (2011) Stem cells in microfluidics. Biomicrofluidics 5:013401
Xia YN, Whitesides GM (1998) Soft lithography. Annu Rev Mater Sci 28:153–184
Yamaguchi N, Torii M, Uebayashi Y, Nasu M (2011) Rapid, semiautomated quantification of bacterial cells in freshwater by using a microfluidic device for on-chip staining and counting. Appl Environ Microbiol 77:1536–1539
Yang M, Li CW, Yang J (2002) Cell docking and on-chip monitoring of cellular reactions with a controlled concentration gradient on a microfluidic device. Anal Chem 74:3991–4001
Yu HB, Zhou GY, Chau FS, Sinha SK (2011) Soft lithography replication based on PDMS partial curing. Microsyst Technol 17:443–449
Zhang B, Kim MC, Thorsen T, Wang ZH (2009) A self-contained microfluidic cell culture system. Biomed Microdevices 11:1233–1237
Acknowledgments
The authors sincerely thank Ministry of Science and Technology of China (No. 2010CB933901), Deutsche Forschungsgesellschaft (International Research Training Group “Materials and Concepts for Advanced Interconnects and Nanosystems”), and Science and Technology Innovation fund of SJTU-University of Michigan.
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Chen, J., Chen, D., Yuan, T. et al. Microfluidic chips for cells capture using 3-D hydrodynamic structure array. Microsyst Technol 20, 485–491 (2014). https://doi.org/10.1007/s00542-013-1933-6
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DOI: https://doi.org/10.1007/s00542-013-1933-6