Abstract
The effects of high pressure on the interactions between volatile anesthetics and three kinds of phosphatidyl choline liposomes were investigated by fluorometry, using a thiacarbocyanine dye (3,3′-dioctadecyl-2,2′-thiacarbocyanine) which is sensitive to the environmental viscosity and dielectric constant. Seven general anesthetics, halothane, enflurane, isoflurane, methoxyflurane, sevoflurane, diethylether and chloroform were used. We have previously reported that these anesthetics decreased the phase transition temperature and increased the effective dielectric constant of the water-liposome interface using dipalmitoyl-phosphatidylcholine. In this study, it was confirmed that the effects of anesthetics on the effective dielectric constant were not altered by the use of a gel or liquidcrystal membrane, and were reversed by the application of high pressure (<800 bars). The increase of the effective dielectric constant was attributed to the perturbation of hydrogen bonds at the liposomal interface. High pressure is considered to promote hydration. Our results obtained under high pressure supported previous observations made at ambient pressure, which suggested that the perturbation of hydrogen bonds at the water-liposome interface correlates with the mechanism of anesthesia.
Similar content being viewed by others
References
Johnson FH, Brown DES, Marsland DA: Pressure reversal of the action of certain narcosis. J Cell Comp Physiol 20:269–276, 1942
Johnson FH, Flagler EA: Hydrostatic pressure reversal of narcosis in tadpoles. Science 112:91–92, 1950
Johnson SM, Miller KW: Antagonism of pressure and anesthesia. Nature 228:75–76, 1970
Lever MJ, Miller KW, Paton WDM, et al: Pressure reversal of anesthesia. Nature 231:368–371, 1971
Meyer HH: Zur Theorie der Alkoholnarkose, I, Mitt welche Eigenschaft der Anasthetika bedingt ihre narkotische Wirkung? Arch Exp Pathoi Pharmakol 42:109–118, 1899
Overton E: Studien uber die Narcose zugleich ein Beitrag zur allgemeinen Pharmakologie. Verlag von Gustav Fischer, Jena, 1901
Tsukamoto I, Yokono S, Shirakawa Y, et al: Interactions between volatile anesthetics and dipalmitoyl phosphatidylcholine liposomes as studied by fluorometry with a thiacarbocyanine dye. J Anesth 6:38–44, 1992
Craig NC, Bryant GJ, Levin IW: Effects of halothane on dipalmitoylphosphatidylcholine liposomes: A Raman spectroscopic study. Biochemistry 26:2449–2458, 1987
Kamaya H, Veda I, Moore PS, et al: Antagonism between high pressure and anesthetics in the thermal phase-transition of dipalmitoyl phosphatidylcholine bilayer. Biochim Biophys Acta 550:131–137, 1979
Kaneshina S, Kamaya H, Veda I: Thermodynamics of pressureanesthetic antagonism on the phase transition of lipid membranes: displacement of anesthetic molecules. J Colloid Interface Sci 93:215–224, 1983
Ferguson J: The use of chemical potentials as indices of toxicity. Proc Roy Soc B 127:387–404, 1939
Nakagaki M, Komatsu H, Handa T: Microenvironment around thiacarbocyanine dyes in lysolecithin micelles, surfactant micelles and lecithin liposomal membranes. Chern Pharm Bull 34:4486–4493, 1986
Dipaolo T, Sandorfy C: Hydrogen bond breaking potency of fluorocarbon anesthetics. J Med Chern 17:809–814, 1974
Tsai Y, Ma S, Kamaya H, et al: Fourier transform infrared studies on phospholipid hydration: Phosphateoriented hydrogen bonding and its attenuation by volatile anesthetics. Mol Pharmacol 31:623–630, 1987
Yoshida T, Okabayashi H, Takahashi K, et al: A proton nuclear magnetic resonance study on the release of bound water by inhalation anesthetic in water-in-oil emulsion. Biochim Biophys Acta 772:102–107, 1984
Veda I, Mashimo T: Anesthetics expand partial molal volume of lipidfree protein dissolved in water: electrostriction hypothesis. Physiol Chern & Physics 14:157–164, 1982
Huckel E: Zur Theorie konzentrierterer wasseringer Losungen starker Elektrolyte. Phys Z, 26:93–147, 1925
Mushayakarara EC, Wong PTT, Mantsch HH: The effect of pressure on the hydrogen bonding between carbonyl and hydroxyl moieties in 1,2-dipalmitoylglycerol: a Fourier-transform infrared spectroscopy study. Biochim Biophys Acta 857:259–264, 1986
Author information
Authors and Affiliations
About this article
Cite this article
Tsukamoto, I., Yokono, S. & Ogli, K. Effects of high pressure on polarity change of the water-liposome interface induced by volatile anesthetics. J Anesth 6, 433–438 (1992). https://doi.org/10.1007/s0054020060433
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s0054020060433