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Interactions between volatile anesthetics and dipalmitoyl phosphatidylcholine liposomes as studied by fluorometry with a thiacarbocyanine dye

Journal of Anesthesia volume 6, pages 38–44 (1992)Cite this article

Abstract

The effects of volatile anesthetics on the properties of dipalmitoyl phosphatidylcholine liposome were investigated by fluorescence spectroscopy with a thiacarbocyanine dye (3,3′-dioctadecyl-2,2′-thiacarbocyanine) which is sensitive to the viscosity and the dielectric constant of the environment. Seven volatile anesthetics, halothane, enflurane, isoflurane, methoxyflurane, sevoflurane, diethylether and chloroform were used. All anesthetics decreased the phase transition temperature of the liposome and increased the effective dielectric constant of the water-liposome interface. The increase of the effective dielectric constant was attributed to the release of the hydrated water molecules from the membrane surface. The increment of the effective dielectric constant depended on the thermodynamic activity of anesthetics in the solution, and was not affected seriously by the kind of anesthetics. On the other hand, the degree of the depression of the phase transition temperature depended on the molar concentrations of anesthetics. Considering from the Ferguson’s report, which is dealt with the relationship between the physiological effect and the thermodynamic activity, the effect of anesthetics on the effective dielectric constant of the membrane surface is more correlated to the anesthetic action than the effect on the phase transition temperature.

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Affiliations

  1. Department of Anesthesiology and Emergency Medicine, Kagawa Medical School, Ikenobe, Mikicho, 761-07, Kitagun, Kagawa, Japan

    Ikuko Tsukamoto, Satoshi Yokono, Yoichi Shirakawa, Hiroyuki Kinoshita, Hisao Komatsu, Mayuki Aibiki & Kenji Ogli

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  1. Ikuko Tsukamoto
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  2. Satoshi Yokono
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  3. Yoichi Shirakawa
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  4. Hiroyuki Kinoshita
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  5. Hisao Komatsu
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  6. Mayuki Aibiki
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  7. Kenji Ogli
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Tsukamoto, I., Yokono, S., Shirakawa, Y. et al. Interactions between volatile anesthetics and dipalmitoyl phosphatidylcholine liposomes as studied by fluorometry with a thiacarbocyanine dye. J Anesth 6, 38–44 (1992). https://doi.org/10.1007/s0054020060038

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  • DOI: https://doi.org/10.1007/s0054020060038

Key words

  • anesthesia theory
  • volatile anesthetics
  • model membrane
  • fluorescence spectroscopy
  • polarity of interface