The effect of sevoflurane on retinal angiogenesis in a mouse model of oxygen-induced retinopathy
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Sevoflurane is commonly used in general anesthesia for premature neonates. The main mechanism of retinopathy of prematurity (ROP) is increased levels of vascular endothelial growth factor (VEGF). For the investigation of sevoflurane’s effect on angiogenesis, the angiogenesis and VEGF expression in the retina were measured after administering sevoflurane in an oxygen-induced retinopathy mice model.
Materials and methods
The mice were divided into the normoxic group (Nc and Ns group; n = 6) and the ROP group (C, Rc, and Rs group; n = 6). Rc group were exposed to 75% oxygen for 5 days beginning on postnatal day (P) 7, and then returned to room air. Age-matched mice in the C group were exposed to room air. To observe angiogenesis of the retina, the mice were sacrificed on P16. The Rs group was exposed to 2 vol% sevoflurane for 2 h on P12, P13, and P14 with 40% oxygen.
The angiogenic area and the spreading distance of vessels on P4 were statistically decreased in the Ns group, compared to the Nc group. The avascular area on P16 was significantly increased and the expression of VEGF was suppressed in the Rs group compared to the Rc group.
Sevoflurane can inhibit retinal angiogenesis via suppressing VEGF expression in an OIR mice model with exposure to relative hypoxia. Nevertheless, it is still difficult to apply the results of this study immediately to humans because of the heterogeneity of responses to sevoflurane.
KeywordsAngiogenesis Oxygen Retinopathy of prematurity Sevoflurane Vascular endothelial growth factor
The authors thank the Department of Pharmacology, Gene and Cell Therapy Center for Vessel-associated Disease, Medical Research Institute, Pusan National University School of Medicine, Yangsan, Republic of Korea for the excellent technical assistance.
HYK, S-HB, SSB, JMH, and MK: study design and data analysis. HYK, S-HB, SWB, SSB, JMH, G-JB, HJK, H-SR, and SHK: manuscript preparation.
Compliance with ethical standards
Conflict of interest
The authors declare that they have competing interests.
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