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Fig. 3 | Journal of Gastroenterology

Fig. 3

From: The pivotal role of SFRP2 in promoting glycolysis and progression in the high-risk group based on the glycometabolism prognostic model for colorectal cancer

Fig. 3

SFRP2 promotes the migration and metastasis of CRC cells in vivo. A Western blot analysis of the expression of β-catenin, Cyclin D1, C-Myc, and phosphorylated GSK-3B after the knockdown or overexpression of SFRP2 in CRC cells. B The mRNA levels of Cyclin D1 in CRC cells with SFRP2 knockdown or overexpression. C Cell migration in SFRP2-knockdown and SFRP2-overexpressing cells was determined by Transwell migration assays. D Western blot analysis of the expression of E-cadherin, Vimentin and Twist after the knockdown or overexpression of SFRP2 in CRC cells. E Measurement of pyruvate and lactate concentrations in the culture media of SFRP2-knockdown and SFRP2-overexpressing cells by ELISA. F Western blot analysis of the expression of β-catenin in cells cultured in media containing different concentrations of SFRP2 or blocking agents of SFRP2. G Cell migration in cells cultured in media supplemented with different concentrations of SFRP2 or blocking agents of SFRP2 was determined by Transwell migration assays. H SW480 cells expressing the scrambled vector or SFRP2 LvRNA and LoVo cells expressing the scrambled vector or SFRP2 shRNA were used to establish the lung metastasis model. Representative image of mice with lung metastasis in the two groups using in vivo bioluminescence imaging and fluorescence intensity. J The overall survival of the mice. K Representative HE images of metastatic lungs and the number of metastatic nodules. *P < 0.05; **P < 0.01; ***P < 0.001; ns. not significant; n = 3

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