The role of diet on intestinal microbiota metabolism: downstream impacts on host immune function and health, and therapeutic implications
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Dietary impacts on health may be one of the oldest concepts in medicine; however, only in recent years have technical advances in mass spectroscopy, gnotobiology, and bacterial sequencing enabled our understanding of human physiology to progress to the point where we can begin to understand how individual dietary components can affect specific illnesses. This review explores the current understanding of the complex interplay between dietary factors and the host microbiome, concentrating on the downstream implications on host immune function and the pathogenesis of disease. We discuss the influence of the gut microbiome on body habitus and explore the primary and secondary effects of diet on enteric microbial community structure. We address the impact of consumption of non-digestible polysaccharides (prebiotics and fiber), choline, carnitine, iron, and fats on host health as mediated by the enteric microbiome. Disease processes emphasized include non-alcoholic fatty liver disease/non-alcoholic steatohepatitis, IBD, and cardiovascular disease/atherosclerosis. The concepts presented in this review have important clinical implications, although more work needs to be done to develop fully and validate potential therapeutic approaches. Specific dietary interventions offer exciting potential for nontoxic, physiologic ways to alter enteric microbial structure and metabolism to benefit the natural history of many intestinal and systemic disorders.
KeywordsShort-chain fatty acids Iron Diet Microbiome IBD NASH NAFLD Fat Atherosclerosis Choline Carnitine
Inflammatory bowel diseases
Irritable bowel syndrome
Non-alcoholic fatty liver disease
Short-chain fatty acids
Diet has long been considered a primary factor in health; however, with the microbiome revolution of the past decade, we have begun to understand how diet can alter the microbial composition and function of a given host . An appreciation of the role of the host microbial community in mucosal homeostasis and a variety of diseases has developed alongside our understanding of the complexities of the microbiome-host interaction.
Perhaps the best example of the microbiome-host interaction in a disease state is in the pathogenesis of inflammatory bowel diseases (IBD), which is believed to be the result of an imbalanced reaction between the genetically regulated host immune system and the microbiome, with important environmental influences . Recent advances suggest that the genetic background of the host may influence the host’s microbiome, with a downstream impact on an individual’s susceptibility to develop IBD, and the severity of their disease . In particular, the rise of IBD incidence in Asia as diet and lifestyle have become more Westernized  demonstrates the profound effects that the microbiome can have on a patient’s health. Similarly, metagenomic/metaproteomic studies have found that ileal Crohn’s disease (CD) patients have a unique signature in both bacterial and host expression profiles .
This review explores the current understanding of interplay between dietary factors and the host microbiome, concentrating on the downstream implications on host immune function and the pathogenesis of disease. In some of these examples, the interplay between all of these various components has not been established. In others, the kinetics of microbial community shifts need further mechanistic exploration to determine whether the change in microbial status is pathogenic, supportive of pathology, or merely a downstream result of a disease state.
The role of various dietary elements on the pathogenesis of gastrointestinal illnesses
Oral, but not parental, iron exacerbates experimental colitis. Results in human IBD are controversial, but IV iron may be more easily tolerated
Lack of SCFA-producing bacteria is associated with IBD, and butyrate supplementation, replacement of selected bacteria or feeding prebiotics offer potential treatments
Experimental murine colitis and human UC patients have decreased levels of serum choline and increased levels bacterial products of choline metabolism
Milk fat promotes a bloom of the colitogenic bacteria Bilophila wadsworthia in experimental murine colitis through increased levels of taurocholic acid
Omega-3 fatty acids
Reduced Th17-mediated inflammation and disease severity in multiple murine models of colitis. Inconsistent results in human IBD studies
A diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAP) reduced IBS symptoms
Gluten alters barrier function in IBS-D patients, particularly those with HLA-DQ2/8 immune-typing, leading to worse symptoms
Dietary choline deficiency predisposes patients to NAFLD/NASH, particularly in those patients with mutations affecting endogenous phosphatidylcholine synthesis or with microbiomes with excessive metabolic breakdown of choline
Choline and carnitine are both metabolized by the microbiome to TMA, which is metabolized by the host to atherosclerosis-promoting TMAO
A high-fiber diet is not protective against asymptomatic diverticulosis
Alcohol is a risk factor for chronic diverticulosis, with increasing consumption enhancing risk
Celiac disease is widely known to be caused by an immune intolerance to gluten, which occurs in a genetically susceptible host
Diet, global microbial communities, and body habitus
Recently, the concept of enterotypes has developed as a means to categorize the microbiomes of large populations of humans. In a study of the fecal metagenomes of individuals from 4 countries across multiple continents, it was proposed that the microbial community structure and molecular functions of a population could be clustered into three distinct categories, or enterotypes . Interestingly, the phylogenic profile of an enterotype was a poor predictor of host physiology, such as body mass index (BMI); however, various genes and functional (metabolic) modules do correlate with host physiology properties such as age and BMI . Subsequent studies have questioned the presence of three broad enterotypes, but the concept of different enteric profiles in human populations is established .
How a given microbial community responds to diet, the role of host genetics in supporting microbial community development, and the effects of different bacterial populations and metabolites on a host’s health all still remain to be determined, but the concept of functionally distinct microbial communities in human populations has presented a conceptual focus as the questions are explored . Based on the diet of individuals, Wu et al. found that normal subjects could be divided into two communities using the same classification as Arumugam et al. : a Bacteroides-dominated profile associated with a high saturated fat/animal protein diet, and a Prevotella-predominant group associated with low amounts of saturated fats and animal proteins but a high amount of carbohydrates and simple sugars . This study also found that the Bacteroides enterotype was resistant to switching to a Prevotella profile during a 10-day feeding trial of a low-fat/high-fiber diet, suggesting that only long-term dietary changes are able to influence enteric bacterial community structure .
A study looking at global bacterial populations in vegans, vegetarians, and control subjects found that vegans had decreased levels of Bacteroides spp., Bifidobacterium spp., Escherichia coli, and Enterobacteriaceae spp. compared to control patients, with vegans serving as an intermediate population . Another study reported that short-term (5-day) diets stratified into animal-based verses plant-based influenced host microbial composition. Animal-based diets increased the abundance of bile-tolerant organisms such as Alistipes, Bilophila, and Bacteroides, and decreased the level of Firmicutes that metabolize dietary plant polysaccharides such as Roseburia, Eubacterium rectale, and Ruminoccus bromii . Interestingly, shifting to a plant-based diet had little effect on microbial composition over this time frame . However, gnotobiotic murine studies demonstrate very rapid shifts in bacterial gene expression following dietary manipulation .
Given the interplay between diet and the host microbiome, the role of the microbiome in obesity has, not surprisingly, been actively explored in recent years . Studies in mice  and humans  have found that an obese state is marked by shifts in the microbiome, specifically a decrease in bacterial diversity with a marked decrease in the abundance of Bacteroidetes and an increased abundance in either Actinobacteria  or Firmicutes ; lean subjects were found to have the opposite trend. Study of carbohydrate metabolic profiles in the human subjects showed clustering along the same Bacteroidetes and Actinobacteria/Firmicutes profiles as was seen with obesity; subjects with high levels of Bacteroidetes were enriched in bacterial carbohydrate metabolism enzymes, while subjects with high level of Firmicutes demonstrated enrichment in transport systems . This study also demonstrated that while a core microbiome consistent between all subjects (obese and lean) was not present, a core set of metabolic functions in the microbiome could be identified . Interestingly, some of this work in part contradicts later studies which demonstrate that a high fat/protein diet was associated with increased levels of the Bacteroides (a genus of the Bacteroidetes phylum); however, this later study looked at diet, and not end phenotype (lean vs. obese body habitus) .
Beyond association studies, functional studies in mice have led to some understanding of how the microbiome can shape body habitus. Germ-free (sterile) mice were found to be resistant to obesity when fed a high-fat diet, mediated by elevated levels of fasting-induced adipose factor (Fiaf) induction of peroxisomal proliferator-activated receptor coactivator (Pgc-1alpha) and increased AMP-activated kinase (AMPK) activity . Additionally, the microbiota of ob/ob mice were found to have an increased capacity to harvest energy from the diet. This property was transferable; germ-free lean mice gavaged with an obese-mouse microbiota developed increased total body fat compared to mice colonized with lean-mouse microbiota .
Follow-up studies in non-obese and obese Danish individuals confirmed that obese individuals had decreased levels of microbial diversity, but this population had increased levels of the Bacteroidetes phylum . This study also explored genetic diversity of the microbiome as the independent variable, instead of BMI. It was found that, independent of weight/BMI, individuals with low bacterial genetic diversity were found to have higher levels of insulin resistance, higher levels of serum triglycerides, cholesterol, and insulin, as well as elevated inflammatory markers (hsCRP and WBC) . Additionally, low bacterial genetic diversity individuals had a shift in microbiota favoring an increase in inflammatory microbe profiles and a decrease in anti-inflammatory microbes such as F. prausnitzii ; even more interestingly, low bacterial-genetic diversity individuals experienced increased weight/BMI gain compared to high diversity individuals, even after controlling for baseline BMI/weight . This difference in weight gain was associated with decreased levels of eight species of bacteria, all but one of which lacked species-specific taxonomic assignment, but all of which were identified as butyrate producers. This work suggests, for the first time, that specific bacterial species could predispose the individual to weight gain, and that differences in the microbiome and bacterial metabolome of obese individuals might be involved in the pathogenesis of obesity, rather than being a consequence of it.
Dietary components also regulate bacterial gene transcription, with bacteria responding to use the available nutrients most optimally. In particular, when gnotobiotic mice were monoassociated with the human organism Bacteroides thetaiotaomicron, this microbe was found to express genes involved in glycan metabolism differently in response to either a polysaccharide-rich or simple-sugar diet to use the energy source available to them most efficiently . For example, the simple sugar diet up-regulated glycoside hydrolase, as well as enzymes able to digest host mucus . Mechanistic studies found that α-mannosides induced the hybrid two-component system BT3172, an environmental sensor that also served as a molecular scaffold for the glycolysis enzymes glucose-6-phosphate isomerase and dehydrogenase  providing a mechanistic example for a how a dietary component can regulate bacterial metabolism.
To add to the complexity of the microbiome, during the past decade, researchers have begun to explore how viruses, and in particular, bacteriophages, sculpt the microbiome . This work has largely been limited both by our understanding of the microbiome as a whole, and the sequencing and analytical tools available to researchers, which only in recent years have become powerful enough to explore viral metagenomics . Interestingly, in patients, CD is associated with higher mucosal bacteriophage counts, suggesting that the virome may be involved in the dysbiosis seen in IBD . In the last year, studies of Bacteroidales-like phage populations in humans have found that, like their microbial hosts, these phages can also be clustered into viral enterotypes , extending the concept of the global microbial structure to the entire microbial content of the gut.
Non-digestible polysaccharides (butyrate production)
Short-chain fatty acids (SCFAs) are produced by fermentation of non-digestible carbohydrates by a subset of anaerobic bacteria in the human colon, primarily in the cecum and proximal colon . Fermentable carbohydrates include pectins, hemicelluloses, gums, and prebiotic substances including fructose and galactose-oligosaccharides that are not responsive to mammalian enzymes, but not what we typically think of as dietary fiber, such as cellulose or wheat bran [23, 24]. The SCFAs produced by fermentation of these dietary substrates serve as a vital fuel source for the colonic epithelium and key regulators of immune homeostasis, and serve as the prototypical example of the symbiotic nature between the microbiome and the host in terms of diet and metabolism . Of the SCFAs, butyrate in particular is considered to be the preferred fuel of colonocytes [23, 25].
During intestinal diversions that result from ostomy procedures, patients develop mild inflammation of the distal, diverted colon, known as diversion colitis. Initial trials in humans demonstrated that butyrate enemas for 2–6 weeks were able to treat patients with diversion colitis , suggesting that diversion colitis may be the result of an absence of the metabolically vital SCFAs. While a follow-up trial of only 2 weeks of treatment did not recapitulate the results of the initial study , more recent studies in rats did replicate the initial findings . In the treatment of ulcerative colitis (UC), butyrate enemas have shown some promise in a subset of patients. Most studies have had positive or largely positive results, with butyrate enemas decreasing rectal inflammation [29, 30, 31, 32], particularly in cases of 5-ASA and steroid-refractory disease . While one randomized, prospective study had negative results , another had mixed findings, with outcomes trending towards significance, and suggesting that efficacy was tied to the length of exposure to the butyrate enema therapy —mirroring the different results seen in the human studies of diversion colitis.
The mechanisms by which butyrate promotes mucosal health remain to be fully determined. Besides being an important colonic epithelial fuel source , it also modulates the immune system in multiple ways. Initial studies using primary human leukocytes found that butyrate inhibits IL-12 production by S. aureus-stimulated human monocytes . The same study also found that in anti-CD3 stimulated monocytes, butyrate enhanced IL-10 and IL-4 secretion, but inhibited IL-2 and IFN-γ release , presenting an anti-inflammatory profile for butyrate. Other in vitro studies have found that butyrate inhibits vascular cell adhesion molecule (VCAM-1)-mediated leukocyte adhesion to endothelial cells . Ex vivo studies in mice found that butyrate suppresses colonic immune activation through Fas-mediated apoptosis of T-cells, including through histone deacetylase (HDAC) 1-dependent Fas up-regulation . This work also provided evidence that butyrate inhibits IFN-γ-mediated inflammatory signaling, particularly through STAT1 and iNOS, and that loss of butyrate signaling induces increased expression of inflammatory genes in mice . Other in vitro findings demonstrate that butyrate inhibits the IFN-γ/STAT1 axis , which is important because enhanced activation of STAT1 occurs in CD patients [38, 40]. Human ex vivo studies found that butyrate was able to decrease pro-inflammatory cytokine (TNF-β, IL-1β, IL-6) mRNA expression as well as TNF secretion in intestinal biopsies and peripheral blood mononuclear cells of CD patients, through inhibition of NF-κB . More recently, studies in murine bone marrow derived macrophages found that n-butyrate was able to inhibit pro-inflammatory cytokine signaling through HDAC inhibition, a process independent of toll-like receptor or G-protein-coupled receptor signaling . This effect has also been observed in in vitro rectal carcinoma cell culture experiments . Several recent studies demonstrated that SCFAs augment colonic regulatory T-cells (Treg) populations, an action mediated in part through a SCFA sensing GPCR encoded by the gene Ffar2 , and whose downstream signaling involved decreases in specific histone deacetylases and net increases in histone acetylation . Arpila and colleagues showed that both butyrate and proprionate, which share HDAC-modulating activity, but not acetate, promote extrathymic Treg generation through the intronic enhancer CNSI . This observation is supported by other work where butyrate directly induced Treg differentiation in the colon and attenuated onset of experimental colitis . These activities are thought to be mediated through GPM109A receptors, which jointly bind butyrate and niacin . These observations are highly relevant to human IBD, since one group of 17 human Clostridium species that produce butyrate and other SCFA can inhibit experimental colitis .
Based on the role of the microbiome in the production of butyrate, work has also been undertaken to determine if patients with IBD have a microbiome that predisposes them to butyrate deficiency. Initial studies found that a subset of patients with IBD had decreased amounts of the phyla Firmicutes and Bacteroidetes , the dominant distal gut phyla and known butyrate producers [50, 51]. The butyrate-producing Clostridum Group IV taxa/subphyla was also implicated in IBD dysbiosis . However, this study did not establish if the microbiome shifts were the result of intestinal inflammation, or were a predisposing factor helping lead to the IBD state.
The first individual bacterial species of these phylum associated with IBD was found to be Faecalibacterium prausnitzii, a Firmicutes and member of the Clostridium Group IV. Patients with recurrent CD after resection were found at the time of resection exam to have decreased amounts of Faecalibacterium prausnitzii, a butyrate producer, suggesting that decreased F. prausnitzii may contribute to or be a marker of a dysbiotic state that predisposes a person to IBD . Follow-up studies in mice found that oral administration of live F. prausnitzii or its supernatant was able to ameliorate TNBS-induced colitis in mice, by partially correcting the dysbiotic state of TNBS-colitis and by inhibiting NF-κB and IL-8 activity .
Follow-up studies have since confirmed that both CD  and UC  patients have decreased levels of butyrate producers, in particular, Clostridum Group IV members and specifically F. prausnitzii. In the UC patients, decreased levels of SCFAs (but oddly not butyrate, which only had a trend towards a significant decrease with disease) were also seen, but the decreases observed were not associated with decreases in a specific bacterial species . Disease activity was inversely correlated with the amount of F. prausnitzii present in the patients’ stool . R. hominis, another butyrate-producing Firmicutes, was also found to be decreased in UC patients, with levels inversely correlated with disease activity . Interestingly, even UC patients in remission had decreased levels of SCFA producers of the Clostridium Group IV, regardless of geographic region , suggesting that part of host susceptibility to UC may be a genetic background or dietary factors that diminish colonization of intestinal track by butyrate producers.
Outside of IBD, there also appears to be an association between decreased levels of butyrate producers in other diarrheal diseases. C. difficile infection, the most common cause of antibiotic-associated diarrhea, with over a half million cases of diarrhea in the United States alone each year and a 30-day mortality of 13.8 % , is associated with decreased levels of these protective bacteria [52, 56]. Even more interestingly, C. difficile-negative nosocomial diarrhea is also associated with a decrease in butyrate-producing bacteria, suggesting that the overall integrity of the epithelial barrier is dependent on sufficient levels of butyrate in the intestinal tract . In a small study of CD patients, oral butyrate showed promising results inducing remission and improving symptoms , suggesting that oral supplementation may be able to overcome the dysbiotic state IBD patients live with.
Choline is a vital component of cell membranes, typically obtained from red meat and eggs, but which can also be biosynthesized [58, 59]. It is central to lipid metabolism, and plays a role in the synthesis of very-low-density lipoprotein (VLDL) in the liver . Recent studies have implicated deficiencies in choline in the development of NAFLD and enhanced progression to NASH. NAFLD occurs in 20–30 % of the general population, while the prevalence in obese patients is 75–100 % [60, 61]. Although patients with NAFLD remain asymptomatic, 20 % progress to NASH, which results in increased mortality through cirrhosis, hepatocellular carcinoma, and portal hypertension [62, 63, 64].
Mice fed diets deficient in methionine-choline develop a NASH phenotype, and this process was negatively regulated by the NLRP6 and NLRP3 inflammasomes as well as their effector protein IL-18 . This phenotype was associated with changes in the composition of the gut microbiota, specifically, increased amounts of Erysipelotrichi. The enhanced liver inflammation seen in the various knockout strains was transmissible to co-housed WT mice . The enhanced severity in inflammasome-deficient mice was mediated by toll-like receptors 4 and 9 through TNF-α, and enhanced levels of TLR4 and 9 agonists were found in the portal circulation of inflammasome-defective mice also deficient in choline intake, likely as a result of sub-clinical colonic inflammation mediated by CCL5 . Interestingly, some of these same observations have been found in human subjects. Patients fed a diet deficient in choline had increased accumulation of hepatic fat and the most susceptible patients were those with increased amounts Erysipelotrichi and decreased levels of Gammaproteobacteria in their stool at baseline .
The genetic background of mice (including SNPs) is an important sculptor of the gut microbiota [58, 66, 67, 68]. A comparison study between two inbred mouse strains, one resistant to NAFLD (BALB/c) and one that is susceptible (129S6) when fed a high fat diet, found that the susceptible strain had lower levels of plasma phosphatidylcholine and higher levels of urinary methylamines . These methylamines are produced exclusively by the gut microbiota as a downstream metabolite of choline. Thus, the microbial composition, as determined by host genetics (and environment), alter the downstream bioavailability of a metabolite (choline) with health consequences for the host . A similar effect of genetics on microbiota was also observed in humans; the PEMT polymorphism increases susceptibility to NAFLD through decreased phosphatidylcholine synthesis. In humans on a normal diet, the abnormal PEMT allele was associated with exacerbated NAFLD in patients with increased stool levels of Erysipelotrichi and decreased levels of Gammaproteobacteria . Patients with the normal allele but with the NAFLD-prone microbial composition had lower levels of hepatic steatosis on a normal diet, suggesting that patients with intact phosphatidylcholine synthesis were able to overcome a NAFLD-inducing gut microbial composition while patients with the PEMT polymorphism were not .
Interestingly, the aforementioned NLRP6 deficient mice, which show enhanced NASH in the absence of dietary choline, also develop exacerbated colitis in response to DSS through increased CCL5 secretion . This phenotype, like that of the NASH model, is also transmittable through the microbiota to wild-type hosts, as the altered microbiota generated by the NLRP6−/− intestine are responsible for increased CCL5 secretion . Choline has not been implicated in this model, however. Additionally, in the murine IL-10−/− model of spontaneous colitis, which is dependent on the presence of commensal microbiota [71, 72], decreased levels of plasma choline were observed, alongside increased choline in phospholipids typical of inflammatory processes . An increase in the urine concentration of trimethylamine (TMA), a gut microbial metabolite of choline, was also observed in IL-10−/− mice, particularly as disease progressed over time , suggesting that the decreased plasma levels of choline in IL-10−/− mice may be the result of increased microbial metabolism of dietary choline. Interestingly, deficiencies in choline are also observed in the colonocytes of patients with active UC when compared to UC patients with quiescent disease . However, the role of choline deficiency in experimental colitis and human IBD remains to be determined.
Enteric bacterial metabolism of choline has also been implicated in CVD and the generation of atherosclerosis. While it has traditionally been thought that saturated fats and red meat were associated with an increased risk of CVD, recent meta-analyses have found no correlation between saturated fat consumption  or red meat consumption  and CVD; only processed meats were noted to be correlated . In humans, three metabolites of phosphatidylcholine: choline, TMA-N-oxide (TMAO), the human metabolite of bacteria-produced TMA ), and betaine, were found to predict CVD in a cohort study . In Apoe−/− atherosclerosis-prone mice, dietary supplementation with choline or TMAO promoted atherosclerosis, which was associated with increased plasma levels of TMAO . These diets were also associated with increased levels of macrophage foam-cells, and increased amounts of expression of the macrophage scavenger receptors CD36 and SR-A1, both of which are implicated in atherosclerosis . Atherosclerosis was positively correlated with increased plasma levels of TMAO, and increased expression of the hepatic enzyme FMO3 that converts TMAs to TMAOs . Because choline produces TMAOs through the bacteria-metabolite intermediate TMAs, the authors hypothesized that the microbiome played a central role in driving choline-induced atherosclerosis. Not surprisingly, germ-free mice did not produce increased levels of TMAO when fed a high-choline diet, and antibiotic decrease of resident bacteria reduced plasma levels of TMAO and prevented dietary-choline-induced macrophage foam cell production and atherosclerosis .
Interestingly, patients with IBD have an increased risk of CVD, despite having a general lower incidence of traditional risk factors [80, 81, 82]. Based on the current literature, microbial choline metabolism may be the missing link explaining this association. IBD has recently been associated with high levels of urine TMA, indicating increased choline metabolism [73, 74, 75] and decreased choline bio-availability. Similarly, increased levels of the downstream metabolite of TMA, TMAO, is associated with increased atherosclerosis . This suggests that the dysbiosis associated with IBD may define a microbiome that favors choline metabolism, decreasing choline bioavailability and increasing downstream atherosclerotic metabolites.
The role of dietary carnitine in the development of atherosclerosis appears very similar to that of choline. l-carnitine is an abundant nutrient in red meat, which like choline, is processed by the enteric microbiota into TMAs [83, 84]. In a recent study using the same metabolomics data set analyzed in the choline study , plasma levels of l-carnitine were also associated with CVD . Using a radio-labeled l-carnitine challenge test, Koeth et al. found that broad spectrum antibiotics diminished the metabolic conversion of l-carnitine to the pro-atherosclerotic metabolite TMAO , indicating a vital role for the microbiome in carnitine-associated atherosclerosis. Furthermore, they found that vegans and vegetarians had lower levels of plasma carnitine and a markedly reduced capacity to synthesize TMAO after a carnitine challenge. These differences in ability to metabolize l-carnitine, as measured by TMAO levels, were associated with several microbial taxa. Additionally, using the aforementioned enterotype classification , the Prevotella enterotype was associated with higher plasma levels of TMAO than the Bacteroides enterotype . Germ-free and antibiotic administration studies in mice confirmed that intestinal microbial metabolism of carnitine to TMA is central to carnitine-enhanced atherosclerosis, and follow-up studies in an independent cohort of human patients found a dose-dependent relationship between plasma carnitine levels and CVD . Mechanistically, increased levels of TMAOs were associated with decreased levels of reverse cholesterol transport, through alterations at multiple sites of the bile acid synthetic pathway [79, 83].
Dietary and supplemental iron
Iron supplementation serves as a prototypical example of the newly discovered complex interactions between diet, the host immune system, and the gut microbiome, which has downstream consequences for gut homeostasis and health. Iron deficiency is the most common cause of anemia worldwide, and oral iron supplementation is commonly prescribed for patients diagnosed with anemia . Many patients with IBD have iron deficiency anemia, in addition to frequent anemia of chronic disease, and are consequently given oral and/or parenteral iron supplementation as part of their treatment course [86, 87]. Clinically, it has long been observed that oral iron supplementation is poorly tolerated by some IBD patients, and anecdotally there were some concerns that symptoms of IBD patients worsened while on oral iron therapy [88, 89]. Iron is a known pro-oxidative agent, raising concern that oral iron may increase oxidative stress in patients with colitis as a mechanism of exacerbating disease .
Because of these concerns, rodent studies have examined the interplay between intestinal inflammation and iron supplementation. Initial studies in both an iodoacetamide rat model of colitis  and in a DSS murine model of colitis  demonstrated that oral ferrous sulfate administration exacerbated disease. Initial mechanistic studies suggested that iron supplementation increased tissue oxidative stress , perhaps through increased tissue iron deposition . Follow-up in vitro studies in a human colonic tumor cell line supported the hypothesis that iron supplementation enhanced reactive oxygen species (ROS) formation, which could lead to increased tissue damage . Proteomic analysis of the effects of iron supplementation in the TNFΔARE/WT model of spontaneous, chronic ileitis (driven by overproduction of TNF) demonstrated that iron supplementation exacerbated disease through contrarily regulated proteins involved in energy homeostasis, host defense, and oxidative and endoplasmic reticulum (ER) stress responses . These proteins were aconitase 2, catalase, intelectin 1, and fumarylacetoacetate hydrolase (FAH) .
Dysfunction of the unfolded protein response (UPR) and downstream autophagic processes as a result of ER stress has been implicated in the pathogenesis of human IBD . Dysfunction of XBP1 protein in mice , as well as the downstream UPR effector ATF6 in both mice  and zebrafish , appear to be involved in the pathogenesis of colitis. One of the key drivers of ER stress in enterocytes is bacterial signaling and translocation across the epithelial barrier . The IBD-susceptibility gene ATG16L1 in combination with murine norovirus infection has been reported to alter Paneth cell function, and is also important for autophagy, the primary response to ER stress . Paneth cells generate numerous antimicrobial peptides and are fundamental to shaping the gut microbial environment . Similarly, NOD2, an intracellular bacterial sensor protein, is also a known IBD-susceptibility gene, with dysfunctional mutations leading to defects in autophagy and resulting intestinal inflammation [97, 99, 100]. Interestingly, oral iron seems to alter these same ER stress/UPR pathways found to be critical in IBD pathogenesis. In the TNFΔARE/WT model of chronic ileitis, oral, but not parental iron, exacerbated disease and enhanced downstream UPR and apoptotic signaling . While oral iron deprivation did not alter T-cell numbers or function, it appeared to sensitize epithelial cells to T-cell (CD8αβ+)-induced apoptosis . Additionally, the lack of oral iron intake in mice altered the microbial composition of both WT and TNFΔARE/WT mice in a similar fashion, independent of the presence of inflammation . Interestingly, while composition was altered, the lack of oral iron intake did not result in changes to overall microbial diversity as measured by the Shannon’s diversity index, nor the number of total operational taxonomic units .
Together, these studies suggest that oral iron intake alters gut microbial composition and perhaps function. These alterations either alone or in parallel with direct induction of ROS by iron lead to increased cell stress in enterocytes with subsequent sensitization to inflammatory damage. Thus, during an acute IBD flare, additional of oral iron, which is commonly prescribed today, may actually exacerbate disease. Based on this body of work, parental iron should be considered for actively flaring IBD patients, with oral supplementation (which is slow to replete iron reserves in any case) used when a patient’s disease is quiescent.
A landmark study recently evaluated the influence of milk fat on the microbiome in murine colitis. Wild-type mice fed a high fat diet comprising either saturated milk fat (MF diet) or polyunsaturated safflower oil fat (PUFA diet) had higher abundances of the Bacteroidetes phylum and decreased levels of Firmicutes, while mice fed a low-fat diet (LF diet) showed the opposite pattern . This pattern mirrored that of the enterotypes seen in human dietary studies . Interestingly, in both the IL-10−/− and DSS models of colitis, the MF diet, but not the PUFA diet, exacerbated disease . This was associated with an increase in Bilophila wadsworthia, a sulfite-reducing bacteria that produces H2S, a substance known to drive T-cell activation and increase gut permeability, and which has been implicated as a bacterial toxin in UC [102, 103, 104]. In this work, mono-association studies with germ-free mice demonstrated that milk fat was needed to support colonization of B. wadsworthia, and that this specific bacteria was colitogenic, through induction of a TH1 immune response involving IFN-γ and IL12 production . Production of taurine-conjugated bile acids (taurocholic acids), a preferred fuel source of B. wadsworthia , is driven by the consumption of hydrophobic fats such as present in the MF diet , and was found to be the key metabolite supporting the bloom of B. wadsworthia in these studies . This study may explain some of the observations in the 1960s that milk consumption was inversely correlated with disease activity in UC patients  and increased milk protein antibodies , but that follow-up studies exploring the presence of milk allergy as an etiology for IBD were largely negative . Additionally, B. wadsworthia levels were increased in the animal-based, short-term diet study , providing evidence that high-fat diets can induce similar changes in microbial changes in humans to those seen in mice. However, the pathogenicity of B. wadsworthia in human IBD remains to be explored.
Not all fat is harmful, however. In mice n-3 polyunsaturated fatty acids (PUFAS, commonly known as omega-3 fatty acids) enhance 5-ASA-mediated recovery from TNBS-colitis , reduce obesity-associated Th17 inflammation during TNBS-colitis , reduce cytokine production and disease severity in T-cell transfer colitis , and reduce Th17 cell populations and disease severity in DSS colitis . In contrast, transfats appear to exacerbate DSS colitis through up-regulation of Th17 signaling . Unfortunately, the results in human CD patients have been more mixed, with conflicting studies finding that omega-3 fatty acids either decreased the rate of relapse , or had no effect on the remission rate .
IBD patients frequently identify dietary components as triggers of increased symptoms and, therefore, may adopt rather rigid dietary preferences based on their individual experiences . These empiric observations, however, are unique to individuals and need to be more thoroughly investigated by integrated studies of the intestinal microbiome, metagenome, and metabolome. Significant mechanistic advances have been made regarding iron [67, 90, 91, 101] and milk fat  in animal models of colitis and ileitis, providing for the first time experimental evidence to back up long-discussed anecdotal observations of diet and IBD. Many IBD patients empirically adopt dairy-free diets , but it is not clear whether this is due to lactose deficiency, milk protein allergy, or intolerance of other milk components. A new insight is provided by the observation that milk fat can induce colitis in genetically susceptible IL-10 deficient mice by selectively altering bile acid profiles and stimulating expansion of a rare anaerobic bacteria, B. wadsworthia . Future studies should target these bile acid and bacterial profiles in human studies, particularly comparing milk-tolerant and -intolerant IBD patients. Oral iron can be inflammatory at doses relevant to Western diets and supplements prescribed to patients [67, 90, 91, 101]. Many IBD patients are anemic and are given therapeutic iron, often during a flare, with improved quality of life after improving hemoglobin levels . Although some clinical studies indicate better clinical tolerance with intravenous (i.v.) compared to oral iron supplementation , this is not universally supported  and the majority of clinicians continue to use oral iron supplementation, although the use of i.v. iron therapy is increasing in some but not all locations [119, 120]. This important clinical issue needs to be resolved with a more comprehensive analysis of the microbiome and fecal metabolome in those patients tolerant vs. intolerant to oral iron therapy to determine a predictive model of optimal supplementation.
IBD patients also frequently include red meats on their list of food intolerances . Whether this perceived intolerance is due to the presence of fats, iron, l-carnitine or other components is unknown. The microbiome-metabolite TMA, which appears to drive diverse inflammatory pathologies, ranging from IBD [73, 74, 75] to atherosclerosis [79, 83], through the host-produced TMA metabolite TMAO. Dietary consumption of phosphatidylcholine and l-carnitine provide substrates for resident enteric bacterial metabolism to TMA, which is then converted in the liver to TMAO. The processes involved in establishing and maintaining a high-TMA-producing microbiome remain to be determined, but host genetics appear to be a contributing factor [75, 79, 83]. Elegant animal and human studies have defined this pathway in atherosclerosis [79, 83]. Further studies should target specific microbial targets to block this metabolic conversion, in parallel with dietary intervention studies. Outside of atherosclerosis and IBD, selective choline supplementation may be helpful in patients with NAFLD/NASH. Similarly, once more is understood about the specific microbes that convert choline/carnitine into TMA, targeted antibiotic therapy could be used to decrease these organisms. Doing so would help to both prevent NAFLD/NASH and the atherosclerosis resulting from UC dysbiosis, as the liver would receive more beneficial dietary choline while simultaneously decreasing the production of atherosclerotic TMA.
While TMA is an example of a harmful bacterial metabolite, other protective metabolites produced by a subset of enteric microbiota, including groups IV and XIVA Clostridium species and F. prausnitzii [49, 52, 53, 56], are reproducibly decreased in IBD and many other inflammatory disorders, and serve as potential therapeutic candidates. These bacteria metabolize nondigestible dietary carbohydrate (fibers, prebiotics) to SCFAs. SCFAs, in particular, butyrate, have multifaceted protective properties that include inhibiting proinflammatory cytokines through HDAC activity, inducing regulatory Treg, and stimulating colonic epithelial restitution [23, 25, 28, 30, 31, 32, 33, 34, 35, 41, 45, 46, 47, 48, 49, 50, 52, 53, 54, 57]. The volatility and instability of SCFAs limit their direct therapeutic application. However, indirect approaches such as restoring luminal concentrations and biologic activity of protective bacterial subsets by novel probiotics using depleted resident species [17, 48] and dietary substrates such as prebiotics and fiber have considerable nontoxic, physiology potential. In addition, there are other factors, such as omega-3 fatty acids [111, 112, 113], retinoic acid, vitamins A, C, D, and E, which function independent of the microbiome and show some therapeutic benefits, and which could be used as an adjunctive anti-inflammatory agent in patients with IBD and atherosclerosis.
Additional areas of very active and productive clinical investigation are interactions between diet and the microbiome in obesity, Type 2 diabetes, and metabolic syndrome [1, 12]. Obesity is clearly associated with abnormal microbial profiles [10, 11, 12, 13, 14], but results are somewhat inconsistent regarding discrete profiles. For example, one study reported that Bacteroides was negatively associated with obesity , while another study found the opposite trend . Amounts of the protective bacteria F. prausnitzii  were found to be negatively correlated with both IBD  and obesity . Together, these works suggest that a pro-inflammatory profile of bacteria predisposes the host to a variety of metabolic and inflammatory processes. However, ambiguity still persists regarding whether a given dysbiosis is merely correlative or an actual causative factor in obesity, particularly regarding the role of Bacteroides in obesity [8, 14, 16]. This may be in part due to the role that nutrient influx plays in shaping a microbiome , which varies between obese and lean individuals. Of note, these trends also appear among the elderly, with the more frail elderly having less diverse microbiomes, decreased numbers of SCFA producers and increased levels of inflammatory markers such as CRP and IL-6, . Interestingly, in the elderly, the same trends also appeared when the patients were analyzed by diet, suggesting that changes in nutritional input may be generating the microbial changes, with subsequent downstream health impacts . These observations imply that diet and nutritional status may be a key intervention to help the elderly.
Overall, the microbiome field has undergone a massive revolution in the last decade, with the availability of affordable microbiome-wide genetic sequencing and the expansion of germ-free and gnotobiotic mouse technologies. As we begin to identify the factors that lead to the development of bacterial community structure and function and learn how these microbiota and their metabolism of dietary components shape human health and disease, completely new types of physiologic, nontoxic therapies could develop, focusing on sculpting the diet and microbiome to promote health.
JRG acknowledges support from NIH F30DK085906.
Conflict of interest
Jason R. Goldsmith discloses that he is a technical consultant for Protagonist Therapuetics (Milpitas, CA); R. Balfour Sartor is member of the United States Probiotic Council (Dannon and Yakult Companies).
- 4.Ng SC, Tang W, Ching JY, Wong M, Chow CM, Hui AJ, et al. Incidence and phenotype of inflammatory bowel disease based on results from the Asia-pacific Crohn’s and colitis epidemiology study. Gastroenterology. 2013;145(1):158–65 e2.Google Scholar
- 10.David LA, Maurice CF, Carmody RN, Gootenberg DB, Button JE, Wolfe BE, et al. Diet rapidly and reproducibly alters the human gut microbiome. Nature. 2013.Google Scholar
- 17.Sokol H, Pigneur B, Watterlot L, Lakhdari O, Bermudez-Humaran LG, Gratadoux JJ, et al. Faecalibacterium prausnitzii is an anti-inflammatory commensal bacterium identified by gut microbiota analysis of Crohn disease patients. Proc Natl Acad Sci USA. 2008;105(43):16731–6.PubMedCentralPubMedGoogle Scholar
- 36.Saemann MD, Bohmig GA, Osterreicher CH, Burtscher H, Parolini O, Diakos C, et al. Anti-inflammatory effects of sodium butyrate on human monocytes: potent inhibition of IL-12 and up-regulation of IL-10 production. FASEB J: Off Publ Fed Am Soc Exp Biol. 2000;14(15):2380–2.Google Scholar
- 42.Chang PV, Hao L, Offermanns S, Medzhitov R. The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition. Proc Nat Acad Sci. 2014.Google Scholar
- 53.Machiels K, Joossens M, Sabino J, De Preter V, Arijs I, Eeckhaut V, et al. A decrease of the butyrate-producing species Roseburia hominis and Faecalibacterium prausnitzii defines dysbiosis in patients with ulcerative colitis. Gut. 2013.Google Scholar
- 72.Sellon RK, Tonkonogy S, Schultz M, Dieleman LA, Grenther W, Balish E, et al. Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. Infect Immun. 1998;66(11):5224–31 [Epub 1998/10/24].PubMedCentralPubMedGoogle Scholar
- 75.Bjerrum JT, Nielsen OH, Hao F, Tang H, Nicholson JK, Wang Y, et al. Metabonomics in ulcerative colitis: diagnostics, biomarker identification, and insight into the pathophysiology. J Proteome Res. 2009;9(2):954–62.Google Scholar
- 80.Aloi M, Tromba L, Di Nardo G, Dilillo A, Del Giudice E, Marocchi E, et al. Premature subclinical atherosclerosis in pediatric inflammatory bowel disease. J Pediatr. 2012;161(4):589–94 e1.Google Scholar
- 89.Lindgren S, Wikman O, Befrits R, Blom H, Eriksson A, Granno C, et al. Intravenous iron sucrose is superior to oral iron sulphate for correcting anaemia and restoring iron stores in IBD patients: a randomized, controlled, evaluator-blind, multicentre study. Scand J Gastroenterol. 2009;44(7):838–45.PubMedGoogle Scholar
- 114.Okada Y, Tsuzuki Y, Sato H, Narimatsu K, Hokari R, Kurihara C, et al. Trans fatty acids exacerbate dextran sodium sulphate-induced colitis by promoting the up-regulation of macrophage-derived proinflammatory cytokines involved in T helper 17 cell polarization. Clin Exp Immunol. 2013;174(3):459–71.PubMedCentralPubMedGoogle Scholar
- 122.Halmos EP, Power VA, Shepherd SJ, Gibson PR, Muir JG. A diet low in FODMAPs reduces symptoms of irritable bowel syndrome. Gastroenterology. 2013.Google Scholar
- 123.Vazquez-Roque MI, Camilleri M, Smyrk T, Murray JA, Marietta E, O’Neill J, et al. A controlled trial of gluten-free diet in patients with irritable bowel syndrome-diarrhea: effects on bowel frequency and intestinal function. Gastroenterology. 2013;144(5):903–11 e3.Google Scholar
- 124.Peery AF, Barrett PR, Park D, Rogers AJ, Galanko JA, Martin CF, et al. A high-fiber diet does not protect against asymptomatic diverticulosis. Gastroenterology. 2012;142(2):266–72 e1.Google Scholar