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Functional polymorphisms of the cyclooxygenase (PTGS2) gene and risk for gallbladder cancer in a North Indian population

  • Original Article—Liver, Pancreas, and Biliary Tract
  • Published:
Journal of Gastroenterology Aims and scope Submit manuscript

Abstract

Background

Cyclooxygenase-2 (PTGS2) overexpression has been implicated in various cancers. We aimed to evaluate the role of PTGS2 −1195G>A [reference sequence (rs) 689466], −765G>C (rs20417) and +8473T>C (rs5275) polymorphisms in conferring interindividual susceptibility to gallbladder cancer.

Materials and methods

The study included 167 gallbladder cancer cases and 184 controls. Genotyping was done by polymerase chain reaction-restriction fragment length polymorphism. Risk was estimated using unconditional logistic regression.

Results

Significant risk was observed in the presence of PTGS2 −1195GA (P = 0.006; odds ratio = 2.00; 95% confidence interval = 1.2–3.3) and AA genotypes (P = 0.050; odds ratio = 2.98; 95% confidence interval = 1.0–8.9). Combined risk due to GA + AA genotypes was 2.12 (P = 0.002; 95% confidence interval = 1.3–3.3; P-trend = 0.001). Sub-grouping showed a risk due to the PTGS2 −1195(GA + AA) genotype in males (P = 0.007; odds ratio = 2.97; 95% confidence interval = 1.3–6.5), patients without gallstones (P = 0.001; odds ratio = 2.53; 95% confidence interval = 1.4–4.7) and with late-onset gallbladder cancer (P = 0.012; odds ratio = 1.99; 95% confidence interval = 1.1–3.4). Gallbladder cancer patients who used tobacco were at increased risk in the presence of the PTGS2 −765GC genotype (P = 0.018; odds ratio = 2.96; 95% confidence interval = 1.2–7.2).

Discussion

An association of PTGS2 −1195G>A polymorphism with gallbladder cancer, particularly in patients without gallstones, suggests a direct role of PTGS2 in cancer development.

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Acknowledgment

The study was supported by fellowship grants from the Counsel of Scientific and Industrial Research (CSIR), Government of India.

Conflict of interest statement

The author(s) declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Genetics, Sanjay Gandhi Post Graduate Institute of Medical Sciences (SGPGIMS), Lucknow, 226014, Uttar Pradesh, India

    Kshitij Srivastava, Anvesha Srivastava, Sachchida Nand Pandey & Balraj Mittal

  2. Department of Surgical Gastroenterology, SGPGIMS, Lucknow, 226014, Uttar Pradesh, India

    Ashok Kumar

Authors
  1. Kshitij Srivastava
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  2. Anvesha Srivastava
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  3. Sachchida Nand Pandey
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  4. Ashok Kumar
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  5. Balraj Mittal
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Corresponding author

Correspondence to Balraj Mittal.

Additional information

K. Srivastava and A. Srivastava contributed equally to the manuscript.

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Srivastava, K., Srivastava, A., Pandey, S.N. et al. Functional polymorphisms of the cyclooxygenase (PTGS2) gene and risk for gallbladder cancer in a North Indian population. J Gastroenterol 44, 774–780 (2009). https://doi.org/10.1007/s00535-009-0071-5

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  • DOI: https://doi.org/10.1007/s00535-009-0071-5

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