The specific purpose of this study was to investigate the effects of medium-chain triglycerides (MCTs) on intestinal cell proliferation and mucous secretion of the small intestine in the rat.
Rats were fed chow diet and given MCTs or the same weight of corn oil (5 g/kg per day) by gavage daily for 2 weeks, and then tissue samples of the small intestines were harvested. Leptin concentration in the small intestine was measured. Cell proliferation and apoptosis in the small intestine was determined by immunohistochemistry. Diamine oxidase (DAO) activity was measured by colorimetric assay.
In rats fed only chow diet (normal rats), the number of goblet cells per villi was 14.2 ± 0.75 in the jejunum and 15.2 ± 1.12 in the ileum. The number of goblet cells increased significantly in rats given MCTs compared with rats given corn oil or normal rats. Ki-67-positive cells were detected on the entire villi and the crypts in the small intestine. Furthermore, the proliferative index and the apoptotic index were also significantly greater in rats given MCTs than rats given corn oil or normal rats. Moreover, DAO activity and leptin concentration in the small intestine were significantly greater in rats given MCTs than rats given corn oil or normal rats.
MCTs enhance cell proliferation of the intestinal epithelium and mucous secretion from goblet cells in the small intestine. These effects may protect the gut in patients suffering from inflammatory bowel disease or enterogenous infection.
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Jordinson M, Goodlad RA, Brynes A, Bliss P, Ghatei MA, Bloom SR, et al. Gastrointestinal responses to a panel of lectins in rats maintained on total parenteral nutrition. Am J Physiol 1999;276:G1235–G1242.
Moriyama K, Kouchi Y, Morinaga H, Irimura K, Hayashi T, Ohuchida A, et al. Diamine oxidase, a plasma biomarker in rats to GI tract toxicity of oral fluorouracil anti-cancer drugs. Toxicology 2006;217:233–239.
Ulusoy H, Usul H, Aydin S, Kaklikkaya N, Cobanoglu U, Reis A, et al. Effects of immunonutrition on intestinal mucosal apoptosis, mucosal atrophy, and bacterial translocation in head injured rats. J Clin Neurosci 2003;10:596–601.
Vetuschi A, Latella G, Sferra R, Caprilli R, Gaudio E. Increased proliferation and apoptosis of colonic epithelial cells in dextran sulfate sodium-induced colitis in rats. Dig Dis Sci 2002;47:1447–1457.
Wolf SE, Debroy MA, Ikeda H, Jeschke M, Matin S, Rajaraman S, et al. Increased small bowel epithelial turnover in interleukin-1 receptor knockout mice. Ann Surg 2000;232:42–45.
Han J, Hamilton JA, Kirkland JL, Corkey BE, Guo W. Mediumchain oil reduces fat mass and down-regulates expression of adipogenic genes in rats. Obes Res 2003;11:734–744.
Kasai M, Maki H, Nosaka N, Aoyama T, Ooyama K, Uto H, et al. Effect of medium-chain triglycerides on the postprandial triglyceride concentration in healthy men. Biosci Biotechnol Biochem 2003;67:46–53.
Nosaka N, Maki H, Suzuki Y, Haruna H, Ohara A, Kasai M, et al. Effects of margarine containing medium-chain triacylglycerols on body fat reduction in humans. J Atheroscler Thromb 2003;10:290–298.
Kono H, Fujii H, Asakawa M, Maki A, Amemiya H, Hirai Y, et al. Medium-chain triglycerides enhance secretory IgA expression in rat intestine after administration of endotoxin. Am J Physiol Gastrointest Liver Physiol 2004;286:G1081–G1089.
Kono H, Fujii H, Asakawa M, Yamamoto M, Matsuda M, Maki A, et al. Protective effects of medium-chain triglycerides on the liver and gut in rats administered endotoxin. Ann Surg 2003;237: 246–255.
Kondo T, Hashi A, Murata S, Fischer SE, Nara M, Nakazawa T, et al. Gastric mucin is expressed in a subset of endocervical tunnel clusters: type A tunnel clusters of gastric phenotype. Histopathology (Oxf) 2007;50:843–850.
Groos S, Reale E, Luciano L. General suitability of techniques for in situ detection of apoptosis in small intestinal epithelium. Anat Rec A Disc Mol Cell Evol Biol 2003;272:503–513.
Matsuura M, Okazaki K, Nishio A, Nakase H, Tamaki H, Uchida K, et al. Therapeutic effects of rectal administration of basic fibroblast growth factor on experimental murine colitis. Gastroenterology 2005;128:975–986.
Muskhelishvili L, Latendresse JR, Kodell RL, Henderson EB. Evaluation of cell proliferation in rat tissues with BrdU, PCNA, Ki-67 (MIB-5) immunohistochemistry and in situ hybridization for histone mRNA. J Histochem Cytochem 2003;51:1681–1688.
Hong MY, Turner ND, Carroll RJ, Chapkin RS, Lupton JR. Differential response to DNA damage may explain different cancer susceptibility between small and large intestine. Exp Biol Med 2005;230:464–471.
Usami M, Ohata A, Kishimoto K, Ohmae K, Aoyama M, Miyoshi M, et al. Phospholipid fatty acid composition and diamine oxidase activity of intestinal mucosa from rats treated with irinotecan hydrochloride (CPT-11) under vegetable oil-enriched diets: comparison between perilla oil and corn oil. JPEN J Parenter Enteral Nutr 2006;30:124–132.
Maxson RT, Dunlap JP, Tryka F, Jackson RJ, Smith SD. The role of the mucus gel layer in intestinal bacterial translocation. J Surg Res 1994;57:682–686.
Verburg M, Renes IB, Meijer HP, Taminiau JA, Buller HA, Einerhand AW, et al. Selective sparing of goblet cells and paneth cells in the intestine of methotrexate-treated rats. Am J Physiol Gastrointest Liver Physiol 2000;279:G1037–G1047.
Plaisancie P, Ducroc R, El Homsi M, Tsocas A, Guilmeau S, Zoghbi S, et al. Luminal leptin activates mucin-secreting goblet cells in the large bowel. Am J Physiol Gastrointest Liver Physiol 2006;290:G805–G812.
Hardwick JCH, Van Den Brink GR, Offerhaus GJ, Van Deventer SJH, Peppelenbosch MP. Leptin is a growth factor for colonic epithelial cells. Gastroenterology 2001;121:79–90.
Spies M, Chappell VL, Dasu MR, Herndon DN, Thompson JC, Wolf SE. Role of TNF-alpha in gut mucosal changes after severe burn. Am J Physiol Gastrointest Liver Physiol 2002;283:G703–G708.
Andersen SN, Rognum TO, Bakka A, Clausen OP. Ki-67: a useful marker for the evaluation of dysplasia in ulcerative colitis. Mol Pathol 1998;51:327–332.
Kullmann F, Fadaie M, Gross V, Knuchel R, Bocker T, Steinbach P, et al. Expression of proliferating cell nuclear antigen (PCNA) and Ki-67 in dysplasia in inflammatory bowel disease. Eur J Gastroenterol Hepatol 1996;8:371–379.
Sjoqvist U, Ost A, Lofberg R. Increased expression of proliferative Ki-67 nuclear antigen is correlated with dysplastic colorectal epithelium in ulcerative colitis. Int J Colorectal Dis 1999;14:107–113.
Shaff RE, Beaven MA. Turnover and synthesis of diamine oxidase (DAO) in rat tissue. Studies with heparin and cycloheximide. Biochem Pharmacol 1976;25:1057–1062.
Takagi K, Nakao M, Ogura Y, Nabeshima T, Kunii A. Sensitive colorimetric assay of serum diamine oxidase. Clin Chim Acta 1994;226:67–75.
Luk GD, Bayless TM, Baylin SB. Diamine oxidase (histaminase). A circulating marker for rat intestinal mucosal maturation and integrity. J Clin Invest 1980;66:66–70.
Lux GD, Marton LJ, Baylin SB. Ornithine decarboxylase is important in intestinal mucosal maturation and recovery from injury in rats. Science 1980;210:195–198.
Buts JP, Theys S, De Keyser N, Dive C. Changes in serum and intestinal diamine oxidase (DAO) activity after proximal enterectomy in rats. Correlation of DAO activity with mucosal mass parameters. Dig Dis Sci 1989;34:1393–1398.
Wollin A, Wang X, Tso P. Nutrients regulate diamine oxidase release from intestinal mucosa. Am J Physiol Regul Integr Comp Physiol 1998;275:969–975.
Wollin A, Jaques LB. Increased diamine oxidase activity in the intestinal lymph of rats on fat ingestion. Can J Physiol Pharmacol 1974;52:760–762.
Iba T, Yagi Y, Kidokoro A, Ohno Y, Kaneshiro Y, Akiyama T. Total parenteral nutrition supplemented with medium-chain triacylglycerols prevents atrophy of the intestinal mucosa in septic rats. Nutrition 1998;14:667–671.
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Ishii, K., Kono, H., Hosomura, N. et al. Medium-chain triglycerides enhance mucous secretion and cell proliferation in the rat. J Gastroenterol 44, 204–211 (2009). https://doi.org/10.1007/s00535-008-2308-0
- goblet cell
- single-stranded DNA
- diamine oxidase