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A better method for confirming Helicobacter pylori infection in Mongolian gerbils

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Abstract

Background. Our aim was to evaluate the accuracy of the stool antigen test and the optimal time point for detecting Helicobacter pylori infection in a Mongolian gerbil model. Methods. We inoculated 8-week-old Mongolian gerbils with H. pylori (Vac A (+)/CagA(+)). The gerbil-infected model was developed as follows: H. pylori was put into broth (about 109 CFU/ml), and 50 gerbils were then fed with 1 ml intragastrically twice within a 3-day interval. Another ten gerbils were fed broth only. Twenty-six weeks after the inoculation, the gerbils were killed. The gastric mucosa was sampled for a series of examinations including culture, histology, rapid urease test, and polymerase chain reaction. Stool samples for a stool antigen test, H. pylori-specific stool antigen assay (HpSA), were collected during weeks 4, 6, 8, 12, and 26 after inoculation. Of the 50 gerbils inoculated with H. pylori, the inoculation was successful in 88%. Severe active gastritis, ulceration, and intestinal metaplasia were obvious. Results. The HpSA test results were sensitivity, 88.6%; specificity, 100%; positive predictive value (PPV), 100%; negative predictive value (NPV), 54.5%, and accuracy, 90%. The HpSA test began to be more sensitive and accurate (P < 0.05) beginning during week 6 after inoculation. We also found that H. pylori could be detected earlier and more easily in the group with high H. pylori density. Conclusions. HpSA seems to be suitable for confirming colonization of gerbils with H. pylori. The optimal testing time point is around 6 weeks after inoculation. This test is a good choice for long-term observation of H. pylori infection in Mongolian gerbils.

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Authors and Affiliations

  1. Division of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan

    Chao-Hung Kuo & Huang-Ming Hu

  2. Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tz-You 1st Road, Kaohsiung, 807, Taiwan

    Chao-Hung Kuo, Chang-Ming Jan, Wen-Ming Wang & Deng-Chyang Wu

  3. Department of Medicine, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

    Chao-Hung Kuo, Pei-Yun Tsai, Jaw-Yuan Wang, Chang-Ming Jan, Wen-Ming Wang & Deng-Chyang Wu

  4. Department of Pathology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan

    Sheau-Fang Yang

  5. Department of Microbiology, Kaohsiung Medical University, Kaohsiung, Taiwan

    Lin-Li Chang

  6. Division of Gastrointestinal Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

    Jaw-Yuan Wang

  7. Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

    Angela Chen

  8. National Sun Yat-Sen University-Kaohsiung Medical University Joint Center, Kaohsiung, Taiwan

    Angela Chen & Deng-Chyang Wu

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  1. Chao-Hung Kuo
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Kuo, CH., Hu, HM., Tsai, PY. et al. A better method for confirming Helicobacter pylori infection in Mongolian gerbils. J Gastroenterol 43, 32–37 (2008). https://doi.org/10.1007/s00535-007-2121-1

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  • DOI: https://doi.org/10.1007/s00535-007-2121-1

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