Abstract
Background
Carvedilol is a nonselective β-blocker with α1-adrenergic blocking activity. It has been shown to decrease portal pressure in cirrhotic patients. The current study was undertaken to evaluate the possible mechanism of carvedilol on hemodynamics in cirrhotic rats with portal hypertension produced by common bile duct ligation.
Methods
Male Sprague-Dawley rats received either a sham operation or common bile duct ligation. Three weeks after surgery, both sham-operated and cirrhotic rats were randomly assigned to receive vehicle or carvedilol 5 mg · kg−1 · 12 h−1 by gastric gavage for 1 week. Hemodynamic measurements, serum biochemistry, serum nitrate/nitrite and 6-keto-PGF1α levels, and aortic mRNA expression of eNOS and COX-1 were performed on the eighth day after drug administration.
Results
Carvedilol treatment did not affect serum biochemistry in either sham-operated or cirrhotic rats. In sham-operated rats, administration of carvedilol significantly decreased the heart rate without affecting other hemodynamic values. In contrast, in cirrhotic rats, administration of carvedilol significantly decreased the cardiac index, portal pressure, heart rate, and portal territory blood flow, and it significantly increased systemic and portal territory vascular resistances. The hepatocollateral resistance was significantly decreased, but the hepatic arterial blood showed no significant changes. In sham-operated rats treated with carvedilol, serum nitrate/nitrite and 6-keto-PGF1α levels were not affected. In contrast, cirrhotic rats receiving carvedilol showed a significant decrease in serum nitrate/nitrite and 6-keto-PGF1α levels, associated with a decrease in aortic mRNA expression of eNOS and COX-1 compared with those receiving vehicle.
Conclusions
Carvedilol decreased portal pressure through a reduction of splanchnic blood flow associated with a decrease in hepatocollateral resistance. Additionally, administration of carvedilol decreased endothelial-related vasodilatory activities.
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References
J Vorobioff JE Bredfeldt RJ Groszmann (1983) ArticleTitleHyperdynamic circulation in portal hypertensive rat model: a primary factor for maintenance of chronic portal hypertension Am J Physiol 244 G52–4 Occurrence Handle6849394 Occurrence Handle1:STN:280:DyaL3s%2Fpsl2ktA%3D%3D
JN Benoit B Zimmerman AJ Premen VLW Go DN Granger (1986) ArticleTitleRole of glucagon in splanchnic hyperemia of chronic portal hypertension Am J Physiol 251 G674–7 Occurrence Handle3777172 Occurrence Handle1:CAS:528:DyaL2sXkvVShuw%3D%3D
N Hori T Okanoue Y Sawa K Kashima (1997) ArticleTitleRole of calcitonin gene-related peptide in the vascular system on the development of the hyperdynamic circulation in conscious cirrhotic rats J Hepatol 26 1111–9 Occurrence Handle9186842 Occurrence Handle1:CAS:528:DyaK2sXjvF2gsro%3D Occurrence Handle10.1016/S0168-8278(97)80120-1
CJ Chu FY Lee SS Wang FY Chang YT Tsai HC Lin et al. (1997) ArticleTitleHyperdynamic circulation of cirrhotic rats: role of substance P and its relationship to nitric oxide Scand J Gastroenterol 32 841–6 Occurrence Handle9282979 Occurrence Handle1:CAS:528:DyaK2sXlsVOqsL8%3D
F Oberti P Sogni S Cailmail R Moreau B Pipy D Lebrec (1993) ArticleTitleRole of prostacyclin in hemodynamic alteration in conscious rats with extrahepatic and intrahepatic portal hypertension Hepatology 18 621–7 Occurrence Handle8359803 Occurrence Handle1:CAS:528:DyaK3sXmsVOjt7g%3D Occurrence Handle10.1016/0270-9139(93)90364-S
MP Pizcueta JM Piqué M Fernández J Bosch J Rodés BJ Whittle et al. (1992) ArticleTitleModulation of the hyperdynamic circulation of cirrhotic rats by nitric oxide inhibition Gastroenterology 103 1909–15 Occurrence Handle1451984 Occurrence Handle1:CAS:528:DyaK3sXitFSmtbk%3D
A Braillon S Cailmail C Gaudin D Lebrec (1993) ArticleTitleReduced splanchnic vasoconstriction to angiotensin II in conscious rats with biliary cirrhosis J Hepatol 17 86–90 Occurrence Handle8445224 Occurrence Handle1:CAS:528:DyaK3sXksVKmu74%3D Occurrence Handle10.1016/S0168-8278(05)80526-4
JF Liao PC Yu HC Lin FY Lee JS Kuo MCM Yang (1994) ArticleTitleStudy on the vascular reactivity and α1-adrenoceptors of portal hypertensive rats Br J Pharmacol 111 439–44 Occurrence Handle7911714 Occurrence Handle1:CAS:528:DyaK2cXhvVeks70%3D
JW Kiel V Pitts JN Benoit DN Granger AP Shepherd (1985) ArticleTitleReduced vascular sensitivity to norepinephrine in portal hypertensive rats Am J Physiol 248 G192–5 Occurrence Handle3970200 Occurrence Handle1:CAS:528:DyaL2MXht12ktbs%3D
D Lebrec (1994) ArticleTitlePharmacological treatment of portal hypertension: hemodynamic effects and prevention of bleeding Pharmacol Ther 61 65–107 Occurrence Handle7938175 Occurrence Handle1:STN:280:DyaK2M%2FisVenuw%3D%3D Occurrence Handle10.1016/0163-7258(94)90059-0
D Lebrec T Poynard P Hillon JP Benhamou (1981) ArticleTitlePropranolol for prevention of recurrent gastrointestinal bleeding in patients with cirrhosis N Engl J Med 305 1371–4 Occurrence Handle7029276 Occurrence Handle1:STN:280:DyaL38%2Flsl2lug%3D%3D Occurrence Handle10.1056/NEJM198112033052302
D Lebrec P Hillon C Muñoz G Goldfarb O Nouel JP Benhamou (1982) ArticleTitleThe effect of propranolol on portal hypertension in patients with cirrhosis: a hemodynamic study Hepatology 2 523–7 Occurrence Handle6981575 Occurrence Handle1:STN:280:DyaL3s%2FgsFertw%3D%3D Occurrence Handle10.1002/hep.1840020502
G Garcia-Tsao ND Grace RJ Groszmann HO Conn MM Bermann MJC Patrick et al. (1986) ArticleTitleShort-term effects of propranolol on portal venous pressure Hepatology 6 101–6 Occurrence Handle3943775 Occurrence Handle1:STN:280:DyaL287gvF2hsQ%3D%3D
J Vorobioff E Picabea R Villavicencio V Puccini O Rossi J Bordato et al. (1987) ArticleTitleAcute and chronic hemodynamic effects of propranolol in unselected cirrhotic patients Hepatology 7 648–53 Occurrence Handle3610045 Occurrence Handle1:STN:280:DyaL2s3nsl2luw%3D%3D
JC García-Pagán M Navasa J Bosch C Bru P Pizcueta J Rodés (1990) ArticleTitleEnhancement of portal pressure reduction by the association of isosorbide-5-mononitrate to propranolol administration in patients with cirrhosis Hepatology 11 230–8 Occurrence Handle2307401
A Albillos JC García-Pagán J Iborra JC Bandi G Cacho M Pérez-Paramo et al. (1998) ArticleTitlePropranolol plus prazosin compared with propranolol plus isosorbide-5-mononitrate in the treatment of portal hypertension Gastroenterology 115 116–23 Occurrence Handle9649466 Occurrence Handle1:CAS:528:DyaK1cXksFSitr4%3D Occurrence Handle10.1016/S0016-5085(98)70372-5
HC Lin YT Tsai MCM Yang LS Chen FY Lee MC Hou et al. (1995) ArticleTitleHemodynamic effects of a combination of propranolol and clonidine in patients with post-hepatitic cirrhosis J Gastroenterol Hepatol 10 281–6 Occurrence Handle7548804 Occurrence Handle1:STN:280:DyaK28%2FitlCguw%3D%3D
F Rodríguez-Pérez RJ Groszmann (1992) ArticleTitlePharmacological treatment of portal hypertension Gastroenterol Clin N Am 21 15–40
D Pateron KA Tazi P Sogni J Heller C Chagneau O Poirel et al. (2000) ArticleTitleRole of aortic nitric oxide synthase 3 (eNOS) in the systemic vasodilatation of portal hypertension Gastroenterology 119 196–200 Occurrence Handle10889169 Occurrence Handle1:CAS:528:DC%2BD3cXlsFGltLk%3D Occurrence Handle10.1053/gast.2000.8554
PA Zweiten (1993) ArticleTitlePharmacodynamic profile of carvedilol Cardiology 2 Suppl 3 19–23 Occurrence Handle10.1159/000175939
EH Forrest IAD Bouchier PC Hayes (1996) ArticleTitleAcute haemodynamic changes after oral carvedilol, a vasodilating beta-blocker, in patients with cirrhosis J Hepatol 25 909–15 Occurrence Handle9007720 Occurrence Handle1:CAS:528:DyaK2sXotlCmtA%3D%3D Occurrence Handle10.1016/S0168-8278(96)80296-0
AJ Stanley G Therapondos A Helmy PC Hayes (1999) ArticleTitleAcute and chronic haemodynamic and renal effects of carvedilol in patients with cirrhosis J Hepatol 30 479–84 Occurrence Handle10190732 Occurrence Handle1:CAS:528:DyaK1MXjtVWqsL8%3D Occurrence Handle10.1016/S0168-8278(99)80108-1
R Bañares E Moitinho B Piqueras M Casado JC García-Pagán A De Diego et al. (1999) ArticleTitleCarvedilol, a new nonselective beta-blocker with intrinsic anti-alpha1-adrenergic activity has a greater portal hypotensive effect than propranolol in patients with cirrhosis Hepatology 30 79–83 Occurrence Handle10385642 Occurrence Handle10.1002/hep.510300124
R Bañares E Moitinho A Matilla JC García-Pagán JL Lampreave C Piera et al. (2002) ArticleTitleRandomized comparison of long-term carvedilol and propranolol administration in the treatment of portal hypertension in cirrhosis Hepatology 36 1367–73 Occurrence Handle12447861 Occurrence Handle10.1053/jhep.2002.36947 Occurrence Handle1:CAS:528:DC%2BD38Xps1ylurw%3D
D Tripathi G Therapondos HF Lui AJ Stanley PC Hayes (2002) ArticleTitleHaemodynamic effects of acute and chronic administration of low-dose carvedilol, a vasodilating β-blocker, in patients with cirrhosis and portal hypertension Aliment Pharmacol Ther 16 373–80 Occurrence Handle11876689 Occurrence Handle1:CAS:528:DC%2BD38XivV2ltL4%3D Occurrence Handle10.1046/j.1365-2036.2002.01190.x
J Kountouras BH Billing PJ Scheuer (1984) ArticleTitleProlonged bile duct obstruction: a new experimental model for cirrhosis in the rat Br J Exp Pathol 65 305–11 Occurrence Handle6743531 Occurrence Handle1:STN:280:DyaL2c3lslChsg%3D%3D
HC Lin MCM Yang MC Hou SM Li YT Huang PC Yu et al. (1996) ArticleTitleEffects of long-term administration of octreotide in portal vein stenosed rats Hepatology 23 537–43 Occurrence Handle8617434 Occurrence Handle1:CAS:528:DyaK28XitlGltLY%3D
A Albillos JC García-Pagán J Iborra JC Bandi G Cacho M Pérez-Paramo et al. (1998) ArticleTitlePropranolol plus prazosin compared with propranolol plus isosorbide-5-mononitrate in the treatment of portal hypertension Gastroenterology 115 116–23 Occurrence Handle9649466 Occurrence Handle1:CAS:528:DyaK1cXksFSitr4%3D Occurrence Handle10.1016/S0016-5085(98)70372-5
C Giannattasio BM Cattaneo G Seravalle S Carugo AA Mangoni G Grass et al. (1992) ArticleTitleα1-Blocking properties of carvedilol during acute and chronic administration J Cardiovasc Pharmacol 19 Suppl 1 S18–22 Occurrence Handle1378144 Occurrence Handle1:STN:280:DyaK38zis1ertQ%3D%3D Occurrence Handle10.1097/00005344-199219001-00005
S Nadaud M Phillippe JF Arnal JB Michel F Soubrier (1996) ArticleTitleSustained increase in aortic endothelial nitric oxide synthase expression in vivo in a model of chronic high blood flow Circ Res 79 857–63 Occurrence Handle8831511 Occurrence Handle1:CAS:528:DyaK28XmtVCmsbk%3D
Grabowski EF, Weksler BB, Jaffe EA. Prostacyclin production by cultured endothelial cells monolayers exposed to step increase in shear stress. J Lab Clin Med 1985;36–43
RJ Kroeger RJ Groszmann (1985) ArticleTitleIncreased portal venous resistance hinders portal pressure reduction during the administration of β-adrenergic blocking agents in a portal hypertensive model Hepatology 5 97–101 Occurrence Handle2857150 Occurrence Handle1:CAS:528:DyaL2MXhtVKjsr0%3D
A Koshy C Girod SS Lee A Hadengue R Cerini D Lebrec (1989) ArticleTitleDiscrepancy between portal pressure and systemic hemodynamic changes after incremental doses of propranolol in awake portal hypertensive rats Hepatology 9 269–73 Occurrence Handle2912829 Occurrence Handle1:CAS:528:DyaL1MXhtVOqs78%3D
NA Flavahan TJ Rimele JP Cooke PM Vanhoutte (1984) ArticleTitleCharacterization of post-junctional alpha-1 and alpha-2 adrenoceptors activated by exogenous or nerve-released norepinephrine in the canine saphenous vein J Pharmacol Exp Ther 230 699–705 Occurrence Handle6088763 Occurrence Handle1:CAS:528:DyaL2cXmtVCiu7o%3D
P Mosca FY Lee AJ Kaumann RJ Groszmann (1992) ArticleTitlePharmacology of portal-systemic collaterals in portal hypertensive rats: role of endothelium Am J Physiol 263 G544–50 Occurrence Handle1415713 Occurrence Handle1:CAS:528:DyaK3sXht1aitLk%3D
HC Lin HL Wu YT Huang MC Hou SD Lee CY Hong (1998) ArticleTitleHaemodynamic effects of 8-day octreotide and prazosin administration in portal hypertensive rats Eur J Clin Invest 28 622–8 Occurrence Handle9767356 Occurrence Handle1:CAS:528:DyaK1cXlvVCnsLk%3D Occurrence Handle10.1046/j.1365-2362.1998.00347.x
A Koshy A Sekiyama A Hadengue R Cerini A Braillon D Lebrec (1992) ArticleTitleEffects of α1 and β-adrenergic antagonists and 5-hydroxytryptamine receptor antagonist on portal-systemic collateral vascular resistance in conscious rats with portal hypertension J Gastroenterol Hepatol 7 449–54 Occurrence Handle1391726 Occurrence Handle1:STN:280:DyaK3s%2FgsVCitA%3D%3D
HJ Grossman VL Grossman PS Bhathal (1992) ArticleTitleEnhanced vasoconstrictor response of the isolated perfused liver preparations from normal and cirrhotic rats J Gastroenterol Hepatol 7 283–7 Occurrence Handle1611016 Occurrence Handle1:STN:280:DyaK38zgsFaiug%3D%3D
P Marteau F Ballet O Chazouilléres Y Chrétien C Rey D Petit et al. (1989) ArticleTitleEffect of vasodilators on hepatic microcirculation in cirrhosis: a study in the isolated perfused rat liver Hepatology 9 820–3 Occurrence Handle2714734 Occurrence Handle1:CAS:528:DyaL1MXktlegtLY%3D
A Albillos JL Lledó R Bañares I Rossi J Iborra JL Calleja et al. (1994) ArticleTitleHemodynamic effects of α-adrenergic blockade with prazosin in cirrhotic patients with portal hypertension Hepatology 20 611–7 Occurrence Handle7915703 Occurrence Handle1:STN:280:DyaK2czlslGnsQ%3D%3D Occurrence Handle10.1016/0270-9139(94)90095-7
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Lin, HC., Huang, YT., Wei, HC. et al. Hemodynamic effects of one week of carvedilol administration on cirrhotic rats. J Gastroenterol 41, 361–368 (2006). https://doi.org/10.1007/s00535-006-1782-5
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DOI: https://doi.org/10.1007/s00535-006-1782-5