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Hepatic effects of influxed endothelin-1 from portal vein: in situ portal vein infusion model using dogs

  • Published:
Journal of Hepato-Biliary-Pancreatic Surgery

Abstract

Background/Purpose

Endothelin-1 is a potent vasoconstrictor formed by vascular endothelium. This study was designed to investigate the hepatic effect of endothelin-1 produced by portal vascular endothelium.

Methods

Portal venous pressure, portal venous flow, hepatic arterial flow, tissue blood flow, and tissue oxygen pressure were measured during portal vein endothelin-1 infusion in dogs at rates of 1.0 to 5.0 ng/kg per minute. Sinusoidal width during maximal infusion was determined morphometrically. Serum concentrations of mitochondrial glutamic oxaloacetic transaminase and endothelin-1 in portal and hepatic venous blood were also measured.

Results

Intraportal endothelin-1 infusion dose-dependently increased portal venous pressure and reduced portal venous and hepatic arterial blood flow. Tissue blood flow and oxygen pressure also decreased. Endothelin-1 also significantly increased serum mitochondrial glutamic oxaloacetic transaminase and constricted hepatic sinusoids. These changes reversed after completion of infusion.

Conclusions

Intraportal endothelin-1 caused circulatory and histological changes in hepatic sinusoids that may suggest the role of endothelin-1 formed by portal venous bed epithelium.

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Authors and Affiliations

  1. First Department of Surgery, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663-8501, Japan

    Nobukazu Kuroda, Junichi Yamanaka, Toshihiro Okada, Tadamichi Hirano, Yuji Iimuro & Jiro Fujimoto

Authors
  1. Nobukazu Kuroda
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  2. Junichi Yamanaka
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  3. Toshihiro Okada
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  4. Tadamichi Hirano
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  5. Yuji Iimuro
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  6. Jiro Fujimoto
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Kuroda, N., Yamanaka, J., Okada, T. et al. Hepatic effects of influxed endothelin-1 from portal vein: in situ portal vein infusion model using dogs. J Hepatobiliary Pancreat Surg 13, 160–166 (2006). https://doi.org/10.1007/s00534-005-1040-x

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  • DOI: https://doi.org/10.1007/s00534-005-1040-x

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