Skip to main content
SpringerLink
Log in

Serum paraoxonase activity, total thiols levels, and oxidative status in patients with acute brucellosis

  • original article
  • Published:
Wiener klinische Wochenschrift Aims and scope Submit manuscript

Summary

It is well known that paraoxonase-1 (PON1) activity may decrease during the course of infection and inflammation. The aim of this study was to investigate serum PON1 activity, oxidative status, and thiols levels in patients with acute brucellosis. In addition, we investigated the PON1 phenotype in patients with acute brucellosis. Thirty patients with acute brucellosis and 35 healthy controls were enrolled. Serum paraoxonase and arylesterase activities, thiols levels, lipid hydroperoxide levels, total antioxidant capacity (TAC), total oxidant status (TOS) and oxidative stress index (OSI) were determined. Serum basal and salt-stimulated paraoxonase–arylesterase activities, TAC levels and thiols levels were significantly lower in patients with acute brucellosis than controls (for all, p < 0.05), while LOOH levels, TOS levels, and OSI values were significantly higher (for all, p < 0.05). We concluded that oxidative stress is increased, while serum PON1 activity is decreased in patients with acute brucellosis. These results indicate that lower PON1 activity is associated with oxidant–antioxidant imbalance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ertek M, Yazgı H, Kadanalı A, Özden K, Ta¸syaran MA. Complications of Brucella infection among adults: an 18-year retrospective evaluation. Turk J Med Sci. 2006;36:377–81.

    Google Scholar 

  2. Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med. 2005;352:2325–36.

    Article  CAS  PubMed  Google Scholar 

  3. Colmenero JD, Reguera JM, Martos F, et al. Complications associated with brucella melitensis infection: a study of 530 Cases. Medicine (Baltimore). 1996;75:195–211.

    Article  CAS  Google Scholar 

  4. Gantt KR, Goldman TL, McCormick ML, et al. Oxidative response of human and murine macrophages during phagocytosis of Leishmania chagasi. J Immunol. 2001;167:893–901.

    Article  CAS  PubMed  Google Scholar 

  5. Dieffenbach CW, Tramont EC. Innate (general or nonspecific) host defense mechanisms. In: Mandell GL, Bennett JE, Dolin R, editors. Principles and practice of infectious diseases. 6. ed. Philadelphia: Elsevier Churchill Livingstone; 2005;34–42.

  6. Melek IM, Erdogan S, Celik S, Aslantas O, Duman T. Evaluation of oxidative stress and inflammation in long term Brucella melitensis infection. Mol Cell Biochem. 2006;293:203–9.

    Article  CAS  PubMed  Google Scholar 

  7. Jiang X, Baldwin CL. Macrophage control of Brucella abortus: role of reactive oxygen intermediates and nitric oxide. Cell Immunol. 1993;151:309–19.

    Article  CAS  PubMed  Google Scholar 

  8. Serefhanoglu K, Taskin A, Turan H, Timurkaynak FE, Arslan H, Erel O. Evaluation of oxidative status in patients with brucellosis. Braz J Infect Dis. 2009;13(4):249–51.

    Article  PubMed  Google Scholar 

  9. Blatter MC, James RW, Messmer S, Barja F, Pometta D. Identification of a distinct human high-density lipoprotein subspecies defined by a lipoprotein-associated protein, K-45. Identity of K-45 with paraoxonase. Eur J Biochem. 1993;211:871–9.

    Article  CAS  PubMed  Google Scholar 

  10. Kosaka T, Yamaguchi M, Motomura T, Mizuno K. Investigation of the relationship between atherosclerosis and paraoxonase or homocysteine thiolactonase activity in patients with type 2 diabetes mellitus using a commercially available assay. Clin Chim Acta. 2005;359:156–62.

    Article  CAS  PubMed  Google Scholar 

  11. Ng CJ, Shih DM, Hama SY, Villa N, NavabM, Reddy ST. The paraoxonase gene family and atherosclerosis. Free Radical Biol Med. 2005;38:153–63.

    Article  CAS  Google Scholar 

  12. Rozenberg O, Rosenblat M, Coleman R, Shih DM, Aviram M. Paraoxonase (PON1) deficiency is associated with increased macrophage oxidative stress: studies in PON1-knock out mice. Free Radical Biol Med. 2003;34:774–84.

    Article  CAS  Google Scholar 

  13. Kotur-Stevuljevic J, Spasic S, Jelic-Ivanovic Z, et al. PON1 status is influenced by oxidative stress and inflammation in coronary heart disease patients. Clin Biochem. 2008;41:1067–73.

    Article  CAS  PubMed  Google Scholar 

  14. Kabaroglu C, Mutaf I, Boydak B, et al. Association between serum paraoxonase activity and oxidative stress in acute coronary syndromes. Acta Cardiol. 2004;59(6):606–11.

    Article  PubMed  Google Scholar 

  15. Cabana VG, Reardon CA, Feng N, Neath S, Lukens J, Getz GS. Serum paraoxonase: effect of the apolipoprotein composition of HDL and the acute phase response. J Lipid Res. 2003;44:780–92.

    Article  CAS  PubMed  Google Scholar 

  16. Karakucuk S, Baskol G, Oner AO, Baskol M, Mirza E, Ustdal M. Serum paraoxonase activity is decreased in the active stage of Behcet’s disease. Br J Ophthalmol. 2004;88:1256–8.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  17. Demirpence O, Sevim B, Yıldırım M, Ayan Nurlu N, Mert D, Evliyaoglu O. Serum paraoxonase, TAS, TOS and ceruloplasmin in brucellosis. Int J Clin Exp Med. 2014;7(6):1592–7.

    CAS  PubMed Central  PubMed  Google Scholar 

  18. Apostolou F, Gazi IF, Kostoula A, et al. Persistence of an atherogenic lipid profile after treatment of acute infection with brucella. J Lipid Res. 2009;50:2532–9.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  19. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radicalcation. Clin Biochem. 2004;37:277–85.

    Article  CAS  PubMed  Google Scholar 

  20. Sodergren E, Nourooz-Zadeh J, Berglund L, Vessby B. Re-evaluation of the ferrous oxidation in xylenol orange assay for the measurement of plasma lipid hydroperoxides. J Biochem Biophys Methods. 1998;37(3):137–46.

    Article  CAS  PubMed  Google Scholar 

  21. Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004;37:112–9.

    Article  CAS  PubMed  Google Scholar 

  22. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005;38:1103–11.

    Article  CAS  PubMed  Google Scholar 

  23. Bolukbas C, Bolukbas FF, Horoz M, Aslan M, Celik H, Erel O. Increased oxidative stress associated with the severity of the liver disease in various forms of hepatitis B virus infection. BMC Infect Dis. 2005;5:95–101.

    Article  PubMed Central  PubMed  Google Scholar 

  24. Eckerson HW, Wyte MC, La Du BN. The human serum paraoxonase/arylesterase polymorphism. Am J Hum Genet. 1983;35:1126–38.

    CAS  PubMed Central  PubMed  Google Scholar 

  25. Haagen L, Brock A. A new automated method for phenotyping arylesterase (E.C. 3.1.1.2) based upon inhibition of enzymatic hydrolysis of 4-nitrophenyl acetate by phenyl acetate. Eur J Clin Chem Clim Biochem. 1992;30:391–5.

    CAS  Google Scholar 

  26. Nourooz Zadeh J. Ferrous ion oxidation in presence of xylenol orange for detection of lipid hydroperoxides in plasma. Methods Enzymol. 1999;300:58–62.

    CAS  PubMed  Google Scholar 

  27. Hu ML. Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol. 1994;233:381–5.

    Google Scholar 

  28. Young EJ. Brucella species. In: Mandell GL, Bennett JE, Dolin R, editors. Principles and practice of infectious diseases. 6. ed. Philadelphia: Elsevier Churchill Livingstone; 2005;2669–74.

  29. Niemela S, Karttunen T, Korhonen T, et al. Could Helicobacter pylori infection increase the risk of coronary heart disease by modifying serum lipid concentrations? Heart. 1996;75:573–5.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  30. Hoffmeister A, Rothenbacher D, Bode G, et al. Current infection with Helicobacter pylori, but not seropositivity to Chlamydia pneumoniae or cytomegalovirus, is associated with an atherogenic, modified lipid profile. Arterioscler Thromb Vasc Biol. 2001;21:427–32.

    Article  CAS  PubMed  Google Scholar 

  31. Altintas E, Ucbilek E, Ulu O, Sezgin O, Uzer C. Helicobacter pylori associated atrophic gastritis and carotid intima-media thickness, is there a link? Int Clin Pract. 2007;61(5):810–4.

    Article  CAS  Google Scholar 

  32. Van Leeuwen, HJ, Heezius EC, Dallinga GM, van Strijp JA, Verhoef J, van Kessel KP. Lipoprotein metabolism in patients with severe sepsis. Crit Care Med. 2003;31:1359–66.

    Article  PubMed  Google Scholar 

  33. Aslan M, Nazligul Y, Horoz M, et al. Serum paraoxonase-1 activity in Helicobacter pylori infected subjects. Atherosclerosis. 2008;196:270–4.

    Article  CAS  PubMed  Google Scholar 

  34. Mackness B, Mackness MI, Arrol S, et al. Serum paraoxonase (PON1) 55 and 192 polymorphism and paraoxonase activity and concentration in non-insulin dependent diabetes mellitus. Atherosclerosis. 1998;139:341–9.

    Article  CAS  PubMed  Google Scholar 

  35. Quemeneur T, Martin-Nizard F, Kandoussi A, et al. PON1, a new biomarker of cardiovascular disease, is low in patients with systemic vasculitis. Semin Arthritis Rheum. 2007;37:149–55.

    Article  CAS  PubMed  Google Scholar 

  36. Mackness MI, Harty D, Bhatnagar D, et al. Serum paraoxonase activity in familial hypercholesterolaemia and insulin-dependent diabetes mellitus. Atherosclerosis. 1991;86:193–9.

    Article  CAS  PubMed  Google Scholar 

  37. Rizzo M, Kotur-Stevuljevic J, Berneis K, Spasojevic- Kalimanovska V, Vekic J. Atherogenic dyslipidemia and oxidative stress: a new look. Transl Res. 2009;153:217–23.

    Article  CAS  PubMed  Google Scholar 

  38. Roberts CK, Sindhu KK. Oxidative stress and metabolic syndrome. Life Sci. 2009;84:705–12.

    Article  CAS  PubMed  Google Scholar 

  39. La Du BN, Aviram M, Billecke S, et al. On the physiological role(s) of the paraoxonases. Chem Biol Interact. 1999;119–120:379–88.

    Article  PubMed  Google Scholar 

  40. Ali EM, Shehata HH, Ali-Labib R, Esmail Zahra LM. Oxidant and antioxidant of arylesterase and paraoxonase as biomarkers in patients with hepatitis C virus. Clin Biochem. 2009;42:1394–1400.

    Article  CAS  PubMed  Google Scholar 

  41. Ates O, Azizi S, Alp HH, Kiziltunc A, Kocer I, Baykal O. Decreased serum paraoxonase 1 activity and increased serum homocysteine and malondialdehyde levels in age-related macular degeneration. Tohoku J Exp Med. 2009;217:17–22.

    Article  CAS  PubMed  Google Scholar 

  42. Kilic SS, Aydin S, Kilic N, Erman F, Aydin S, Celik I. Serum arylesterase and paraoxonase activity in patients with chronic hepatitis. World J Gastroenterol. 2005;11:7351–4.

    CAS  PubMed  Google Scholar 

  43. Parra S, Alonso-Villaverde C, Coll B, et al. Serum paraoxonase-1 activity and concentration are infl uenced by human immunodefi ciency virus infection. Atherosclerosis. 2007;194:175–81.

    Article  CAS  PubMed  Google Scholar 

  44. Aviram M, Rosenblat M, Billecke S, et al. Human serum paraoxonase (PON1) is inactivated by oxidized low density lipoprotein and preserved by antioxidants. Free Radical Biol Med. 1999;26:892–904.

    Article  CAS  Google Scholar 

  45. Dirican M, Akca R, Sarandol E, Dilek K. Serum paraoxonase activity in uremic predialysis and hemodialysis patients. J Nephrol. 2004;17:813–8.

    CAS  PubMed  Google Scholar 

  46. Mackness B, Durington P, McElduff P, et al. Low paraoxonase activity predicts coronary events in the caterphilly prospective study. Circulation. 2003;107:2775–9.

    Article  CAS  PubMed  Google Scholar 

  47. Sutherland WHF, Walker RJ, de Jong SA, van Rij AM, Phillips V, Walker HL. Reduced postprandial serum paraoxonase activity after meal rich in used cooking fat. Arterioscler Thromb Vasc Biol. 1999;19:1340–7.

    Article  CAS  PubMed  Google Scholar 

  48. Van der Gaag MS, van Tol A, Scheek LM, et al. Daily moderate alcohol consumption increases serum paraoxonase activity; a diet controlled, randomized intervention study in middle-aged men. Atherosclerosis. 1999;147:405–10.

  49. Chandra M, Chandra N, Agrawal R, Kumar A, Ghatak A, Pandey VC. The free radical system in ischemic heart disease. Int J Cardiol. 1994;43:121–5.

    Article  CAS  PubMed  Google Scholar 

  50. Jarvik GP, Tsai NT, McKinstry LA, et al. Vitamin C and E intake is associated with increased paraoxonase activity. Arterioscler Thromb Vasc Biol. 2004;22:1329–33.

    Article  Google Scholar 

  51. Kakafika AI, Xenofontos S, Tsimihodimos V, et al. The PON1 M55 L gene polymorphism is associated with reduced HDL-associated PAF-AH activity. J Lipid Res. 2003;44:1919–26.

    Article  CAS  PubMed  Google Scholar 

  52. Aslan M, Kosecik M, Horoz M, Selek S, Celik H, Erel O. Assessment of paraoxonase and arylesterase activities in patientswith iron deficiency anemia. Atherosclerosis. 2007;191:397–402.

    Article  CAS  PubMed  Google Scholar 

  53. Li HL, Liu DP, Liang CC. Paraoxonase gene polymorphisms, oxidative stress, and diseases. J Mol Med. 2003;81(12):766–79.

    Article  CAS  PubMed  Google Scholar 

  54. Sepahvand F, Shafiei M, Ghaffari SM, Rahimi-Moghaddam P, Mahmoudian M. Paraoxonase phenotype distribution in a healthy Iranian population. Basic Clin Pharmacol Toxicol. 2007;101:104–7.

    Article  CAS  PubMed  Google Scholar 

  55. Mackness B, Davies GK, Turkie W, et al. Paraoxonase status in coronary heart disease: are activity and concentration more important than genotype?. Arterioscler Thromb Vasc Biol. 2001;21:1451–7.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medical Faculty, Department of Internal Medicine, Yuzuncu Yıl University, 65000, Van, Turkey

    Ramazan Esen,  Mehmet Aslan & Mehmet Emin Kucukoglu

  2. Medical Faculty, Department of Microbiology, Yuzuncu Yıl University, Van, Turkey

    Aytekin Cıkman

  3. Medical Faculty, Department of Infectious Disease and Clinical Microbiology, Yuzuncu Yıl University, Van, Turkey

    Umit Yakan & Mahmut Sunnetcioglu

  4. Medical Faculty, Department of Clinical Biochemistry, Bezmialem Vakif University, Istanbul, Turkey

    Sahbettin Selek

Authors
  1. Ramazan Esen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mehmet Aslan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mehmet Emin Kucukoglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aytekin Cıkman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Umit Yakan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mahmut Sunnetcioglu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sahbettin Selek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mehmet Aslan.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Esen, R., Aslan, M., Kucukoglu, M. et al. Serum paraoxonase activity, total thiols levels, and oxidative status in patients with acute brucellosis. Wien Klin Wochenschr 127, 427–433 (2015). https://doi.org/10.1007/s00508-015-0720-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00508-015-0720-z

Keywords

Search

Navigation