Skip to main content
Log in

Basic and applied research at the department of cardio-thoracic surgery: work in progress

  • Review Article
  • Published:
Wiener klinische Wochenschrift Aims and scope Submit manuscript

Summary

A departmental history is mirrored by its members and their scientific and clinical output. In honour of Prof. Ernst Wolners retirement the authors were invited to summarize various aspects of basic and applied research performed at the Department of Cardiothoracic Surgery in Vienna within the last decade. This free communications suffices to present a short introduction of work performed by four independent laboratories headed by staff surgeons.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Ankersmit HJ, Wieselthaler G, Moser B, Gerlitz S, Roth G, Boltz-Nitulescu G, et al (2002) Transitory immunologic response after implantation of the DeBakey VAD continuous-axial-flow pump. J Thorac Cardiovasc Surg 123 (3): 557–61

    Article  PubMed  Google Scholar 

  • Ankersmit HJ, Edwards NM, Schuster M, John R, Kocher A, Rose EA, et al (1999) Quantitative changes in T-cell populations after left ventricular assist device implantation: relationship to T-cell apoptosis and soluble CD95. Circulation 100 [19 Suppl]: II211–5

    PubMed  CAS  Google Scholar 

  • Ankersmit HJ, Tugulea S, Spanier T, Weinberg AD, Artrip JH, Burke EM, et al (1999) Activation-induced T-cell death and immune dysfunction after implantation of left-ventricular assist device. Lancet 354 (9178): 550–5

    Article  PubMed  CAS  Google Scholar 

  • Moser B, Roth G, Brunner M, Lilaj T, Deicher R, Wolner E, et al (2003) Aberrant T cell activation and heightened apoptotic turnover in end-stage renal failure patients: a comparative evaluation between non-dialysis, haemodialysis, and peritoneal dialysis. Biochem Biophys Res Commun 308 (3): 581–5

    Article  PubMed  CAS  Google Scholar 

  • Ankersmit HJ, Moser B, Roedler S, Teufel I, Zuckermann A, Roth G, et al (2002) Death-inducing receptors and apoptotic changes in lymphocytes of patients with heart transplant vasculopathy. Clin Exp Immunol 127 (1): 183–9

    Article  PubMed  CAS  Google Scholar 

  • Roth GA, Moser B, Krenn C, Roth-Walter F, Hetz H, Richter S, et al (2005) Heightened levels of circulating 20S proteasome in critically ill patients. Eur J Clin Invest 35 (6): 399–403

    Article  PubMed  CAS  Google Scholar 

  • Brunner M, Krenn C, Roth G, Moser B, Dworschak M, Jensen-Jarolim E, et al (2004) Increased levels of soluble ST2 protein and IgG1 production in patients with sepsis and trauma. Intensive Care Medicine 30 (7): 1468–73

    Article  PubMed  Google Scholar 

  • Roth GA, Krenn C, Brunner M, Moser B, Ploder M, Spittler A, et al (2004) Elevated serum levels of epithelial cell apoptosis-specific cytokeratin 18 neoepitope m30 in critically ill patients. Shock 22 (3): 218–20

    Article  PubMed  CAS  Google Scholar 

  • Roth G, Moser B, Krenn C, Brunner M, Haisjackl M, Almer G, et al (2003) Susceptibility to programmed cell death in T-lymphocytes from septic patients: a mechanism for lymphopenia and Th2 predominance. Biochem Biophys Res Commun 308 (4): 840–6

    Article  PubMed  CAS  Google Scholar 

  • Ankersmit H, Moser B, Teufel I, Hericis R, Grimm M, Boltz-Nitulescu G, et al (2001) Increased death inducing receptors in vivo and activation-induced T-cell death by anti-thymocyte antibody treatment of stable cardiac transplant recipients. J Heart Lung Transplant 20 (2): 187

    Article  PubMed  Google Scholar 

  • Ankersmit HJ, Moser B, Zuckermann A, Roth G, Taghavi S, Brunner M, et al (2002) Activation-induced T cell death, and aberrant T cell activation via TNFR1 and CD95-CD95 ligand pathway in stable cardiac transplant recipients. Clin Exp Immunol 128 (1): 175–80

    Article  PubMed  CAS  Google Scholar 

  • Ankersmit HJ, Moser B, Hoffman M, Kocher AA, Schlechta B, Boltz-Nitulescu G, et al (2001) Aberrant T-cell activation via CD95 and apoptosis in peripheral T lymphocytes in stable heart transplant recipients. Transplant Proc 33 (5): 2860–1

    Article  PubMed  CAS  Google Scholar 

  • Hoetzenecker K, Hacker S, Hoetzenecker W, Sadeghi K, Sachet M, Pollreisz A, et al (2007) Cytomegalovirus hyper-immunoglobulin: mechanisms in allo-immune response in vitro. Eur J Clin Invest 37 (12): 978–86

    Article  PubMed  CAS  Google Scholar 

  • Ankersmit HJ, Roth GA, Moser B, Zuckermann A, Brunner M, Rosin C, et al (2003) CD32-mediated platelet aggregation in vitro by anti-thymocyte globulin: implication of therapy-induced in vivo thrombocytopenia. Am J Transplant 3 (6): 754–9

    Article  PubMed  CAS  Google Scholar 

  • Pollreisz A, Assinger A, Hacker S, Hoetzenecker K, Schmid W, Lang G, et al (2008) Intravenous immunoglobulins induce CD32-mediated platelet aggregation in vitro. Br J Dermatol [Epub ahead of print]

  • Konakci KZ, Bohle B, Blumer R, Hoetzenecker W, Roth G, Moser B, et al (2005) Alpha-Gal on bioprostheses: xenograft immune response in cardiac surgery. Eur J Clin Invest 35 (1): 17–23

    Article  PubMed  CAS  Google Scholar 

  • Soleiman A, Lukschal A, Hacker S, Aumayr K, Hoetzenecker K, Lichtenauer M, et al (2008) Myocardial Lipofuscin-Laden Lysosomes Contain the Apoptosis Marker Caspase-cleaved Cytokeratin-18. (accepted to Eur J Clin Investigation)

  • Adlbrecht C, Hoetzenecker K, Posch M, Steiner S, Kopp C, Hacker S, et al (2007) Elevated levels of interleukin-1beta-converting enzyme and caspase-cleaved cytokeratin-18 in acute myocardial infarction. Eur J Clin Invest 37 (5): 372–80

    Article  PubMed  CAS  Google Scholar 

  • Ankersmit HJ, Weber T, Auer J, Roth G, Brunner M, Kvas E, et al (2004) Increased serum concentrations of soluble CD95/Fas and caspase 1/ICE in patients with acute angina. Heart Feb 90 (2): 151–4

    Article  CAS  Google Scholar 

  • Szerafin T, Hoetzenecker K, Hacker S, Horvath A, Pollreisz A, Arpád P, et al (2008) Heat shock proteins 27, 60, 70, 90alpha, and 20S proteasome in on-pump versus off-pump coronary artery bypass graft patients. Ann Thorac Surg 85 (1): 80–7

    Article  PubMed  Google Scholar 

  • Szerafin T, Horvath A, Moser B, Hacker S, Hoetzenecker K, Steinlechner B, et al (2006) Apoptosis-specific activation markers in on- versus off-pump coronary artery bypass graft (CABG) patients. Clin Lab 52 (5–6): 255–61

    PubMed  Google Scholar 

  • Szerafin T, Brunner M, Horváth A, Szentgyörgyi L, Moser B, Boltz-Nitulescu G, et al (2005) Soluble ST2 protein in cardiac surgery: a possible negative feedback loop to prevent uncontrolled inflammatory reactions. Clin Lab 51 (11–12): 657–63

    PubMed  CAS  Google Scholar 

  • Podesser BK, Hausleithner V, Seitelberger R, Wollenek G, Wolner E, Steiert H (1993) New developments in the isolated working heart. A comparison of neonatal, immature and adult rabbits after sixty minutes of ischemia in respect to hemodynamic and biochemical parameters. Pharmacol Toxicol 30 (4): 189–96

    Article  CAS  Google Scholar 

  • Podesser BK, Wollenek G, Windischbauer A, Zegner M, Hausleitner V, Seitelberger R, et al (1994) Myocardial protection with Bretschneider cardioplegic solution – an evaluation of full oxygenation. Eur Surg Res 26: 133–40

    Article  PubMed  CAS  Google Scholar 

  • Zwölfer W, Podesser BK, Wollenek G, Zegner M, Haider W (1994) Postischemic hemodynamic changes after piroximone administration in isolated rabbit heart. Eur Surg Res 26: 141–8

    Article  PubMed  Google Scholar 

  • Zwölfer W, Podesser BK, Wollenek G, Zegner M, Haider W (1996) Vergleich dreier gängiger Kardioplegielösungen-Untersuchungen am Isolierten Herz zur Myokardprotektion während I/R. Acta chirurgica austriaca 28: 17–28

    Google Scholar 

  • Zegner M, Podesser BK, Koci G, Kunold A, Wollenek G, Wolner E (1996) Bewertung der Reperfusion unter Einfluß von Ramiprilat-Untersuchungen am isolierten "working-heart-Modell". Acta chirurgica austriaca 28: 5–16

    Google Scholar 

  • Podesser BK, Rajek A, Kupilik N, Schmiedberger A, Vukovich Th, Hiesmayr M, et al (1997) Myocardial revascularization without cardiopulmonary bypass in pigs: a comparison between β-blockers and Ca2+-channel blockers. J Cardiovasc Surg 5: 382–7

    Article  CAS  Google Scholar 

  • Podesser BK, Neumann F, Neumann M, Schreiner W, Wollenek G, Mallinger R (1998) Outer radius-wall thickness ratio, a postmortem quantitative histology in human coronary arteries. Acta Anat 163: 63–8

    Article  PubMed  CAS  Google Scholar 

  • Podesser BK, Hallström S, Schima H, Huber L, Weisser J, Kröner A, et al (1999) The erythrocyte-perfused "working heart" model: hemodynamic and metabolic performance in comparison to crystalloid perfused hearts. Pharmacol Toxicol 41: 9–15

    Article  CAS  Google Scholar 

  • Podesser BK, Schirnhofer J, Bernecker OY, Kröner A, Franz M, Semsroth S, et al (2002) Optimizing ischemia-reperfusion in the failing rat heart-improved myocardial protection with acute ACE-inhibition. Circulation 106: I277–I283

    PubMed  Google Scholar 

  • Korn P, Kröner A, Schirnhofer J, Hallström S, Schmiedberger A, Mallinger R, et al (2002) Quinaprilat during cardioplegic arrest prevents ischemia-reperfusion injury. J Thorac Cardiovasc Surg 124: 352–9

    Article  PubMed  CAS  Google Scholar 

  • Kröner A, Seitelberger R, Kunold A, Schirnhofer J, Schmiedberger A, Mallinger R, et al (2002) Diltiazem during reperfusion preserves high energy phosphates by protection of mitochondrial integrity and function. Eur J Cardio-Thorac Surg 21: 224–31

    Article  Google Scholar 

  • Semsroth S, Fellner B, Trescher K, Bernecker OY, Kalinowski L, Gasser H, et al (2005) S-Nitroso Human Serum Albumin attenuates ischemia/reperfusion injury after prolonged cardioplegic arrest in isolated rabbit heart. J Heart Lung Transplant 24 (12): 2226–34

    Article  PubMed  Google Scholar 

  • Hallström S, Franz M, Gasser H, Vodrazka M, Semsroth S, Losert U, et al (2008) S-Nitroso Human Serum Albumin Reduces Ischemia/Reperfusion Injury in the Pig Heart after Unprotected Warm Ischemia. Cardiovasc Res 77 (3): 506–14

    Article  PubMed  CAS  Google Scholar 

  • Trescher K, Bernecker O, Fellner B, Gyöngyösi M, Krieger S, DeMartin R, et al (2004) Adenovirus-mediated overexpression of inhibitor kappa B-alpha attenuates postinfarct remodeling in the rat heart. Eur J Cardio-Thoracic Surg 26 (5): 960–7

    Article  Google Scholar 

  • Trescher K, Bernecker O, Fellner B, Gyöngyösi M, Schäfer R, Aharinejad S, et al (2006) Inflammation and postinfarct remodeling: overexpression of IkappaB prevents ventricular dilation via increasing TIMP levels. Cardiovasc Res 69: 746–54

    Article  PubMed  CAS  Google Scholar 

  • Jain M, Liao R, Podesser BK, Ngoy S, Apstein CS, Eberli FR (2002) Influence of gender on the response to hemodynamic overload after myocardial infarction. Am J Physiol Heart Circ Physiol 283: H2544–50

    PubMed  CAS  Google Scholar 

  • Podesser BK, Jain M, Ngoy S, Apstein CS, Eberli FR (2007) Unveiling gender differences in demand ischemia: a study in a rat model of genetic hypertension. Eur J Cardiothorac Surg 31: 298–304

    Article  PubMed  Google Scholar 

  • Bauer M, Podesser BK (2008) Gender- and Sex-specific Differences in Ischemic Heart Failure, the Role of Estrogen. Gender Medicine (in print)

  • Trescher K, Bauer M, Dietl W, Hallström S, Wick N, Wolner E, et al (2008) Improved myocardial protection in the failing heart by selective endothelin-A receptor blockade. J Thorac Cardiovasc Surg (in print)

  • Abraham D, Hofbauer R, Schafer R, Blumer R, Paulus P, Miksovsky A, et al (2000) Selective downregulation of VEGF-A(165), VEGF-R(1), and decreased capillary density in patients with dilative but not ischemic cardiomyopathy. Circ Res 87: 644–7

    PubMed  CAS  Google Scholar 

  • Aharinejad S, Andrukhova O, Lucas T, Zuckermann A, Wieselthaler G, Wolner E, et al (2008) Programmed cell death in idiopathic dilated cardiomyopathy is mediated by suppression of the apoptosis inhibitor Apollon. Ann Thorac Surg 86: 109–14

    Article  PubMed  Google Scholar 

  • Trescher K, Bernecker O, Fellner B, Gyongyosi M, Schafer R, Aharinejad S, et al (2006) Inflammation and postinfarct remodeling: overexpression of IkappaB prevents ventricular dilation via increasing TIMP levels. Cardiovasc Res 69: 746–54

    Article  PubMed  CAS  Google Scholar 

  • Aharinejad S, Abraham D, Paulus P, Zins K, Hofmann M, Michlits W, et al (2008) Colony-stimulating factor-1 transfection of myoblasts improves the repair of failing myocardium following autologous myoblast transplantation. Cardiovasc Res (in press)

  • Schafer R, Abraham D, Paulus P, Blumer R, Grimm M, Wojta J, et al (2003) Impaired VE-cadherin/beta-catenin expression mediates endothelial cell degeneration in dilated cardiomyopathy. Circulation 108: 1585–91

    Article  PubMed  CAS  Google Scholar 

  • Aharinejad S, Schafer R, Krenn K, Zuckermann A, Schneider B, Neumann F, et al (2007) Donor myocardial HIF-1alpha is an independent predictor of cardiac allograft dysfunction: a 7-year prospective, exploratory study. Am J Transplant 7: 2012–9

    Article  PubMed  CAS  Google Scholar 

  • Aharinejad S, Krenn K, Paulus P, Schafer R, Zuckermann A, Grimm M, et al (2005) Differential role of TGF-beta1/bFGF and ET-1 in graft fibrosis in heart failure patients. Am J Transplant 5: 2185–92

    Article  PubMed  CAS  Google Scholar 

  • Aharinejad S, Schafer R, Hofbauer R, Abraham D, Blumer R, Miksovsky A, et al (2001) Impact of cardiac transplantation on molecular pathology of ET-1, VEGF-C, and mitochondrial metabolism and morphology in dilated versus ischemic cardiomyopathic patients. Transplantation 72: 1043–9

    Article  PubMed  CAS  Google Scholar 

  • Krenn K, Klepetko W, Taghavi S, Lang G, Schneider B, Aharinejad S (2007) Recipient vascular endothelial growth factor serum levels predict primary lung graft dysfunction. Am J Transplant 7: 700–6

    Article  PubMed  CAS  Google Scholar 

  • Taghavi S, Krenn K, Jaksch P, Klepetko W, Aharinejad S (2005) Broncho-alveolar lavage matrix metalloproteases as a sensitive measure of bronchiolitis obliterans. Am J Transplant 5: 1548–52

    Article  PubMed  Google Scholar 

  • Aharinejad S, Taghavi S, Klepetko W, Abraham D (2004) Prediction of lung-transplant rejection by hepatocyte growth factor. Lancet 363: 1503–8

    Article  PubMed  CAS  Google Scholar 

  • Taghavi S, Abraham D, Riml P, Paulus P, Schafer R, Klepetko W, et al (2002) Co-expression of endothelin-1 and vascular endothelial growth factor mediates increased vascular permeability in lung grafts before reperfusion. J Heart Lung Transplant 21: 600–3

    Article  PubMed  Google Scholar 

  • Mohl W (1984) The development and rationale of pressure-controlled intermittent coronary sinus occlusion-a new approach to protect ischemic myocardium. Wien Klin Wochenschr 96 (1): 20–5

    PubMed  CAS  Google Scholar 

  • Neumann F, Mohl W, Schreiner W (1989) Coronary sinus pressure and arterial flow during intermittent coronary sinus occlusion. Am J Physiol 256 (3 Pt 2): H906–15

    PubMed  CAS  Google Scholar 

  • Mohl W, Glogar DH, et al (1984) Reduction of infarct size induced by pressure-controlled intermittent coronary sinus occlusion. J Cardiol 53 (7): 923–8

    Article  CAS  Google Scholar 

  • Syeda B, Schukro C, Heinze G, Modaressi K, Glogar D, Maurer G, et al (2004) The salvage potential of coronary sinus interventions: meta-analysis and pathophysiologic consequences. J Thorac Cardiovasc Surg 127 (6): 1703–12

    Article  PubMed  Google Scholar 

  • Kenner T, Moser M, Mohl W (1985) Arteriovenous difference of the blood density in the coronary circulation. J Biomech Eng 107 (1): 34–40

    PubMed  CAS  Google Scholar 

  • Mohl W, Kenner T, Klepetko W, Moritz A, Moser M, Müller M, et al (2003) Enhancement of washout induced by Pressure Controlled Intermittent Coronary Sinus Occlusion (PICSO) in the canine and human heart. Coronary Sinus Library – ICSO and PICSO, pp 145–56

  • Havel M, Mohl W, Schopf G, Müller MM (1986) Purine nucleotides in human hearts during open heart surgery. Adv Exp Med Biol 195 Pt B: 529–33

    PubMed  Google Scholar 

  • Schopf M, Schuster M, Müller M: Effects of PICSO on purine nucleotides in ischemic canine and reperfused human hearts. Coronary Sinus Library – ICSO and PICSO, pp 124–131

  • Weigel G, Kajgana I, Bergmeister H, Riedl G, Glogar HD, Gyöngyösi M, et al (2007) Beck and back: a paradigm change in coronary sinus interventions – pulsatile stretch on intact coronary venous endothelium." J Thorac Cardiovasc Surg 133 (6): 1581–7

    Article  PubMed  Google Scholar 

  • Mohl W, Kajgana I, Bergmeister H, Rattay F (2005) Intermittent pressure elevation of the coronary venous system as a method to protect ischemic myocardium. Interact Cardiovasc Thorac Surg 4 (1): 66–9

    Article  PubMed  Google Scholar 

  • Mohl W (2007) Embryonic recall: myocardial regeneration beyond stem cell transplantation. Wien Klin Wochenschr 119 (11–12): 333–6

    Article  PubMed  Google Scholar 

  • Mohl W, Mina S, Milasinovic D, Kasahara H, Wei S, Maurer G (2008) Is activation of coronary venous cells the key to cardiac regeneration? Nat Clin Pract Cardiovasc Med [Epub ahead of print]

  • Mohl W, Simon P, Neumann F, Schreiner W, Punzengruber C (1988) Clinical evaluation of pressure-controlled intermittent coronary sinus occlusion: randomized trial during coronary artery surgery. Ann Thorac Surg 46 (2): 192–201

    Article  PubMed  CAS  Google Scholar 

  • Mohl W, Komamura K, Kasahara H, Heinze G, Glogar D, Hirayama A, et al (2008) Myocardial protection via the coronary sinus. Circ J 72 (4): 526–33

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Hendrik J. Ankersmit.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ankersmit, H., Podesser, B., Aharinejad, S. et al. Basic and applied research at the department of cardio-thoracic surgery: work in progress. Wien Klin Wochenschr 120 (Suppl 2), 39–45 (2008). https://doi.org/10.1007/s00508-008-1044-z

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00508-008-1044-z

Keywords

Navigation