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Sexual Plant Reproduction

, Volume 10, Issue 1, pp 49–64 | Cite as

Megagametogenesis in Arabidopsis wild type and the Gf mutant

  • Cory A. Christensen
  • Edward J. King
  • John R. Jordan
  • G. N. Drews
ORIGINAL PAPER

Abstract

 The female gametophyte is an essential structure for angiosperm reproduction that mediates a host of reproductive functions and, following fertilization, gives rise to most of the seed. Here, we describe a rapid method to analyze Arabidopsis female gametophyte structure using confocal laser scanning microscopy (CLSM). We present a comprehensive description of megagametogenesis in wild-type Arabidopsis. Based on our observations, we divided Arabidopsis megagametogenesis into eight morphologically distinct stages. We show that synergid cell degeneration is triggered by pollination, that dramatic nuclear migrations take place during the four-nucleate stage, and that megagametogenesis within a pistil is fairly synchronous. Finally, we present a phenotypic analysis of the previously reported Gf mutant (Redei 1965) and show that it affects an early step of megagametogenesis.

Key words Arabidopsis Confocal laser scanning microscopy Female gametophyte Gf mutant Megagametogenesis Plant reproduction 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • Cory A. Christensen
    • 1
  • Edward J. King
    • 1
  • John R. Jordan
    • 1
  • G. N. Drews
    • 1
  1. 1.Department of Biology, University of Utah, Salt Lake City, UT 84112, USA Tel. +1–801–585–6203; Fax +1–801–581–4668; e-mail drews@bioscience.utah.eduUS

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