Regulation of floral meristem activity through the interaction of AGAMOUS, SUPERMAN, and CLAVATA3 in Arabidopsis
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Floral meristem size is redundantly controlled by CLAVATA3, AGAMOUS , and SUPERMAN in Arabidopsis.
The proper regulation of floral meristem activity is key to the formation of optimally sized flowers with a fixed number of organs. In Arabidopsis thaliana, multiple regulators determine this activity. A small secreted peptide, CLAVATA3 (CLV3), functions as an important negative regulator of stem cell activity. Two transcription factors, AGAMOUS (AG) and SUPERMAN (SUP), act in different pathways to regulate the termination of floral meristem activity. Previous research has not addressed the genetic interactions among these three genes. Here, we quantified the floral developmental stage-specific phenotypic consequences of combining mutations of AG, SUP, and CLV3. Our detailed phenotypic and genetic analyses revealed that these three genes act in partially redundant pathways to coordinately modulate floral meristem sizes in a spatial and temporal manner. Analyses of the ag sup clv3 triple mutant, which developed a mass of undifferentiated cells in its flowers, allowed us to identify downstream targets of AG with roles in reproductive development and in the termination of floral meristem activity. Our study highlights the role of AG in repressing genes that are expressed in organ initial cells to control floral meristem activity.
KeywordsArabidopsis thaliana Floral meristem CLAVATA3 AGAMOUS SUPERMAN Reproductive development
The authors would like to thank Akie Takahashi and Taeko Kawakami for technical assistance, and Elliot Meyerowitz for providing pWUS::GFP-ER lines. This work was supported by Grants from Japan Science and Technology Agency “Precursory Research for Embryonic Science and Technology (No. JPMJPR15QA),” a JSPS KAKENHI (No. 16H01468), the NAIST Foundation, the Sumitomo Foundation, the Takeda Foundation, and the Mishima Kaiun Memorial Foundation to N.Y.; a Grant from JSPS KAKENHI (No. 15H05955) to T. S.; a Grant from Japan Science and Technology Agency “Precursory Research for Embryonic Science and Technology (No. JPMJPR15Q2)” to Y.I.; Grants from JSPS KAKENHI (Nos. 15H05959 and 17H06172) to K.S.; and Grants from the NAIST Foundation, the Mitsubishi Foundation, and JSPS KAKENHI (15H01234, 15H01356, 15H02405, and 17H05843) to T.I.
- Besnard F, Refahi Y, Morin V, Marteaux B, Brunoud G, Chambrier P, Rozier F, Mirabet V, Legrand J, Laine S, Thevenon E, Farcot E, Cellier C, Das P, Bishopp A, Dumas R, Parcy F, Helariutta Y, Boudaoud A, Godin C, Traas J, Guedon Y, Vernoux T (2014) Cytokinin signaling inhibitory fields provide robustness to phyllotaxis. Nature 505:417–421CrossRefPubMedGoogle Scholar
- Breuil-Broyer S, Trehin C, Morel P, Boltz V, Sun B, Chambrier P, Ito T, Negrutiu I (2016) Analysis of the Arabidopsis superman allelic series and the interactions with other genes demonstrate developmental robustness and joint specification of male–female boundary, flower meristem termination and carpel compartmentalization. Ann Bot 117:905–923CrossRefPubMedPubMedCentralGoogle Scholar
- Clark SE, Running MP, Meyerowitz EM (1995) CLAVATA3 is a specific regulator of shoot and floral meristem development affecting the same processes as CLAVATA1. Development 121:2057–2067Google Scholar
- Riechmann JL, Heard J, Martin G, Reuber L, Jiang C, Keddie J, Adam L, Pineda O, Ratcliffe OJ, Samaha RR, Creelman R, Pilgrim M, Broun P, Zhang JZ, Ghandehari D, Sherman BK, Yu G (2000) Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes. Science 290:2105–2110CrossRefPubMedGoogle Scholar
- Townsley BT, Covington MF, Ichihashi Y, Zumstein K, Sinha NR (2015) Brad-seq: breath adapter directional sequencing: a streamlined, ultra-simple and fast library preparation and fast library preparation protocol for strand specific mRNA library construction. Front Plant Sci 6:366CrossRefPubMedPubMedCentralGoogle Scholar
- Winter CM, Austin RS, Blanvillain-Baufume S, Reback MA, Monniaux M, Wu MF, Sang Y, Yamaguchi A, Yamaguchi N, Parker JE, Parcy F, Jensen ST, Li H, Wagner D (2011) LEAFY target genes reveal floral regulatory logic, cis motifs, and a link to biotic stimulus response. Dev Cell 20:430–443CrossRefPubMedGoogle Scholar