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Application of rice microspore-preferred promoters to manipulate early pollen development in Arabidopsis: a heterologous system

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Key message

Rice microspore-promoters.

Abstract

Based on microarray data analyzed for developing anthers and pollen grains, we identified nine rice microspore-preferred (RMP) genes, designated RMP1 through RMP9. To extend their biotechnological applicability, we then investigated the activity of RMP promoters originating from monocotyledonous rice in a heterologous system of dicotyledonous Arabidopsis. Expression of GUS was significantly induced in transgenic plants from the microspore to the mature pollen stages and was driven by the RMP1, RMP3, RMP4, RMP5, and RMP9 promoters. We found it interesting that, whereas RMP2 and RMP6 directed GUS expression in microspore at the early unicellular and bicellular stages, RMP7 and RMP8 seemed to be expressed at the late tricellular and mature pollen stages. Moreover, GUS was expressed in seven promoters, RMP3 through RMP9, during the seedling stage, in immature leaves, cotyledons, and roots. To confirm microspore-specific expression, we used complementation analysis with an Arabidopsis male-specific gametophytic mutant, sidecar pollen-2 (scp-2), to verify the activity of three promoters. That mutant shows defects in microspore development prior to pollen mitosis I. These results provide strong evidence that the SIDECAR POLLEN gene, driven by RMP promoters, successfully complements the scp-2 mutation, and they strongly suggest that these promoters can potentially be applied for manipulating the expression of target genes at the microspore stage in various species.

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Acknowledgements

This work was carried out with the support of the “Cooperative Research Program for Agriculture Science and Technology Development (Project Nos. PJ01182602 to KHJ; PJ01194201 to SKP),” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Korea

    Tien Dung Nguyen, Moe Moe Oo, Rupesh Tayade, Jong Tae Song, Sung Aeong Oh & Soon Ki Park

  2. Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee University, Yongin, 17104, Korea

    Sunok Moon & Ki Hong Jung

  3. Department of Molecular Biology, Sejong University, Seoul, 143-747, Korea

    Moon-Soo Soh

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  1. Tien Dung Nguyen
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  2. Sunok Moon
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  3. Moe Moe Oo
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  9. Soon Ki Park
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Correspondence to Ki Hong Jung or Soon Ki Park.

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Communicated by David Twell.

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Fig. S1

Complementation analysis of scp-2 mutant using SCP gene expression under the control of RMP promoters in a heterologous system. a Schematic diagram of vectors used for complementation analysis. b Silique production in complementing lines (proRMP-SCP) compared with scp-2 hm mutant background (scp-2) and wild-type plants (EPS 8787 kb)

Fig. S2

Confirmation of T-DNA insertion in ProRMP-SCP:dHA lines. a 1316-bp SCP-dHA fragment was amplified from DNA of 14 transgenic lines (Lanes 1–14), using gene-specific primers SCP forward and reverse. b scp-2 allele was detected using LBb1 and LOBRP primer set. c SCP wild-type gene was checked in scp-2 hm background and transgenic lines using LBb1 and 47870-R primers. scp-2 hm and Col ecotype were used as controls (EPS 5240 kb)

Fig. S3

Percentages of aberrant pollen grains from non-transformed scp-2 homozygotes (scp-2) and transformed scp-2 hm harboring proRMP-SCP:dHA were calculated at mature pollen stage in T1 generation (EPS 3269 kb)

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Nguyen, T.D., Moon, S., Oo, M.M. et al. Application of rice microspore-preferred promoters to manipulate early pollen development in Arabidopsis: a heterologous system. Plant Reprod 29, 291–300 (2016). https://doi.org/10.1007/s00497-016-0293-7

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