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The central cell nuclear position at the micropylar end is maintained by the balance of F-actin dynamics, but dispensable for karyogamy in Arabidopsis

Abstract

Key message

Advances in Fertilization.

In flowering plants, fertilization is initiated by the delivery of immotile sperm cells to the boundary between two female gametes, the egg cell and the central cell. During female gamete development in Arabidopsis, the nucleus of the central cell becomes positioned toward this boundary. How this specific polarized nuclear position is maintained is unclear and whether it plays a role in successful fertilization remains to be determined. By disrupting and manipulating F-actin dynamics in the central cell, we identified that the balance of F-actin dynamics is important for nuclear positioning in the central cell and that the presence of intact F-actin cables in the central cell correlates with successful karyogamy regardless of the central cell nuclear position. We also report that the surface of the central cell nucleus is enriched in F-actin. Thus, the central cell nucleus might also serve as a site that organizes F-actin cytoskeleton to promote sperm cell nucleus movement and karyogamy.

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Acknowledgments

TK and FB were funded by Temasek Lifesciences Laboratory and Gregor Mendel Institute. TK received an additional fund from FWF grant P26887-B21.

Conflict of interest

The authors declare that they have no conflict of interest.

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Correspondence to Frederic Berger.

Additional information

A contribution to the special issue ‘From Gametes to Seeds’.

Communicated by Silvia Coimbra and Lucia Colombo.

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Kawashima, T., Berger, F. The central cell nuclear position at the micropylar end is maintained by the balance of F-actin dynamics, but dispensable for karyogamy in Arabidopsis . Plant Reprod 28, 103–110 (2015). https://doi.org/10.1007/s00497-015-0259-1

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  • DOI: https://doi.org/10.1007/s00497-015-0259-1

Keywords

  • Fertilization
  • Gamete
  • F-actin
  • Nuclear migration