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Sexual Plant Reproduction

, Volume 25, Issue 4, pp 319–335 | Cite as

New insights into the variability of reproduction modes in European populations of Rubus subgen. Rubus: how sexual are polyploid brambles?

  • Petra Šarhanová
  • Radim J. Vašut
  • Martin Dančák
  • Petr Bureš
  • Bohumil Trávníček
Original Article

Abstract

Rubus subgen. Rubus includes common European species with highly complicated taxonomy, ongoing hybridisation and facultative apomixis. Out of approximately 750 species recognised in Europe, only 3 diploid sexual species are known, along with numerous apomictic brambles that are highly connected to polyploidy. One exception of a tetraploid taxon is R. ser. Glandulosi, which is known for prevalent sexuality. This taxon highly hybridises with tetraploid members of R. ser. Discolores and leads to the origin of many hybridogenous populations and individuals. In this study, we verify reproduction modes in different diploid, triploid and tetraploid species of subgen. Rubus, with focus on taxa putatively involved in such hybridisation by applying flow cytometric seed screen analysis. We found 100 % sexuality of diploid species, whereas triploid species had obligate unreduced embryo sac development. In contrast, tetraploid plants had varying degrees of sexuality. Additionally, we discovered that R. bifrons has the ability to undergo a reproduction mode switch as a reaction to environmental conditions. These results provide insight into reproductive modes of European brambles and shed light on their reticulate evolution and speciation.

Keywords

Apomixis Endosperm Flow cytometry Hybridisation Rubus 

Notes

Acknowledgments

We thank all colleagues who helped in the fieldwork with finding the localities of bramble species, namely M. Lepší (České Budějovice), P. Lepší (Český Krumlov) and V. Žíla (Strakonice). We also thank Tim Sharbel for comments regarding the manuscript. M. Tesařová and K. Truhlářová (Palacký University, Olomouc) were students who performed part of the confirmatory flow cytometric analyses. This study was supported by the Grant Agency of the Czech Republic (grants no. 206/07/0706 and 206/08/0890) and by the internal grant from Palacký University (PrF 2012/001).

Supplementary material

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Supplementary material 1 (PDF 389 kb)
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Supplementary material 2 (PDF 273 kb)
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Supplementary material 3 (PDF 894 kb)
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Supplementary material 4 (PDF 416 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Petra Šarhanová
    • 1
    • 4
  • Radim J. Vašut
    • 1
  • Martin Dančák
    • 2
  • Petr Bureš
    • 3
  • Bohumil Trávníček
    • 1
  1. 1.Department of Botany, Faculty of SciencePalacký University in OlomoucOlomoucCzech Republic
  2. 2.Department of Ecology and Environmental SciencesPalacký University in OlomoucOlomoucCzech Republic
  3. 3.Department of Botany and ZoologyMasaryk UniversityBrnoCzech Republic
  4. 4.Department of Taxonomy and Evolutionary BiologyLeibniz Institute of Plant Genetics and Crop ResearchGaterslebenGermany

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