Abstract
Pis 30, a gene highly expressed in Brassica napus pistils and encoding a novel proline-rich protein was isolated and characterized. Sequences homologous to the Brassica Pis 30 gene were found only in Arabidopsis thaliana. The Pis 30 gene encodes a mature protein of 8.4 kDa with no previously characterized protein domains and whose function remains unknown. PIS 30 contains especially high levels of Pro (33%), but also of Leu (14%), Phe (10%) and Ser (6%). Although it is a proline-rich protein, PIS 30 shows only limited similarity to previously characterized plant proline-rich proteins. When compared to the stigma-specific activity of the B. napus SLR1 gene promoter in pistils of transgenic Arabidopsis, an 808 bp Pis 30 promoter fragment directed β-glucuronidase expression primarily in the ovary, as well as in the stigma.
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The authors would like to thank Martine Racicot, Serge Gravel, Dina Joyal and Chantal Gaudet for technical assistance, Eric Johnson for flower photography and Dr. David Wilkinson and Dr. Robert Watson for critical review of this manuscript.
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Foster, E., Lévesque-Lemay, M., Schneiderman, D. et al. Characterization of a gene highly expressed in the Brassica napus pistil that encodes a novel proline-rich protein. Sex Plant Reprod 17, 261–267 (2005). https://doi.org/10.1007/s00497-004-0236-6
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DOI: https://doi.org/10.1007/s00497-004-0236-6