Abstract.
Gametophytic self-incompatibility, a natural mechanism occurring in pear and other fruit-tree species, is usually controlled by the S-locus with allelic variants (S1, S2, Sn). Recently, biochemical and molecular tools have determined the S-genotype of cultivars in various species. The present study determined the S-locus composition of ten European pear cultivars via S-PCR molecular assay, thereby obviating time-consuming fieldwork whose results are often ambiguous because of environmental effects. To verify the S-PCR assay, two putative S-allele DNA fragments of Japanese pear were isolated; their sequences proved to be identical to those reported in the databank. Six S-allele fragments of European pear were then sequenced. While field data confirmed the molecular results, fully and half-compatible field crosses were not distinguishable.
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Zuccherelli, S., Tassinari, P., Broothaerts, W. et al. S-Allele characterization in self-incompatible pear (Pyrus communis L.). Sex Plant Reprod 15, 153–158 (2002). https://doi.org/10.1007/s00497-002-0145-5
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DOI: https://doi.org/10.1007/s00497-002-0145-5