Abstract.
Changes in loosely bound calcium of rice egg cells (pre- and post-pollination) and two-celled proembryos were localized using potassium pyroantimonate precipitation. Egg cells and zygotes (6 h after pollination) have few calcium precipitates (ppts); however within 3 h, (9 –12 h after pollination), ppts become locally abundant in all regions. Statistical analysis indicates that ppts in cytoplasm and nucleoplasm increase by 4.4 and 10.5-fold in zygotes at 9 h and 12 h after pollination, respectively. The ppts labeling the nucleolus 9 h after pollination show a 52-fold increase. In two-celled proembryos, ppts declined to a level similar to that of zygotes 6 h after pollination, except in the nucleolus and cell wall. Following pollination, abundant calcium was detected in the apoplast of the embryo sac.
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Zhao, .J., Yu, .F., Liang, .S. et al. Changes of calcium distribution in egg cells, zygotes and two-celled proembryos of rice (Oryza sativa L.). Sex Plant Reprod 14, 331–337 (2002). https://doi.org/10.1007/s00497-002-0127-7
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DOI: https://doi.org/10.1007/s00497-002-0127-7